Fluorophore labeling of native FKBP12 by ligand-directed tosyl chemistry allows detection of its molecular interactions in vitro and in living cells

Journal of the American Chemical Society

Volumn 135, Issue 18, 2013, Pages 6782-6785

  Tamura, Tomonori ^a   Kioi, Yoshiyuki ^a   Miki, Takayuki ^a   Tsukiji, Shinya ^c   Hamachi, Itaru ^a,b  

^a KYOTO UNIVERSITY   (Japan)

^b JAPAN SCIENCE AND TECHNOLOGY AGENCY   (Japan)

^c NAGAOKA UNIVERSITY OF TECHNOLOGY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

CHEMICAL BIOLOGY; FK506-BINDING PROTEINS; FLUORESCENT LABELING; FLUORESCENT REPORTER; IN-VITRO; LIVING CELL; RAPAMYCIN; SITE-SPECIFIC;

BINDING ENERGY; FLUORESCENCE; FLUOROPHORES; LIGANDS; PROTEINS;

CHEMICAL ANALYSIS;

BINDING PROTEIN; FK506 BINDING PROTEIN 12; FLUORESCENT DYE; OREGON GREEN; RAPAMYCIN; UNCLASSIFIED DRUG;

ARTICLE; BINDING SITE; CATALYSIS; CHEMICAL LABELING; COMPLEX FORMATION; CONTROLLED STUDY; FLUORESCENCE ANALYSIS; FLUORESCENCE RESONANCE ENERGY TRANSFER; IMAGE ANALYSIS; IN VITRO STUDY; LIGAND BINDING; LIGAND DIRECTED TOSYL CHEMISTRY; MOLECULAR INTERACTION; PROTEIN BINDING; PROTEIN DOMAIN; PROTEIN TARGETING;

CELL LINE, TUMOR; FLUORESCENT DYES; HUMANS; MOLECULAR STRUCTURE; TACROLIMUS BINDING PROTEIN 1A;

EID: 84877248493     PISSN: 00027863     EISSN: 15205126     Source Type: Journal    
DOI: 10.1021/ja401956b     Document Type: Article

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