Real-time in situ viability assessment in a 3D bioreactor with liver cells using resazurin assay

Cytotechnology

Volumn 65, Issue 2, 2013, Pages 297-305

  Mueller, Daniel ^a   Tascher, Georg ^a   Damm, Georg ^b   Nüssler, Andreas K ^c   Heinzle, Elmar ^a   Noor, Fozia ^a  

^a SAARLAND UNIVERSITY   (Germany)

^b CHARITÉ UNIVERSITÄTSMEDIZIN BERLIN   (Germany)

^c UNIVERSITY OF TÜBINGEN   (Germany)

Author keywords

3D bioreactor; Drug testing; In situ viability assay; Primary hepatocytes; Resazurin

Indexed keywords

ALBUMIN; AMIODARONE; FLUORESCENT DYE; GLUCOSE; MITOCHONDRIAL ENZYME; RESAZURIN; UREA;

ARTICLE; BIOREACTOR; CELL STRESS; CELL VIABILITY; COCULTURE; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; CYTOPLASM; CYTOTOXICITY; GLUCOSE INTAKE; HUMAN; HUMAN CELL; LIVER CELL; LIVER TOXICITY; THREE DIMENSIONAL IMAGING; UREA CYCLE;

EID: 84876799377     PISSN: 09209069     EISSN: 15730778     Source Type: Journal    
DOI: 10.1007/s10616-012-9486-6     Document Type: Article

References (18)

1. Abu-Absi SF, Friend JR, Hansen LK, Hu WS (2002) Structural polarity and functional bile canaliculi in rat hepatocyte spheroids. Exp Cell Res 274:56-67
2. Altmann B, Giselbrecht S, Weibezahn KF, Welle A, Gottwald E (2008) The three-dimensional cultivation of the carcinoma cell line HepG2 in a perfused chip system leads to a more differentiated phenotype of the cells compared to monolayer culture. Biomed Mater 3:034120
3. Bokhari M, Carnachan RJ, Cameron NR, Przyborski SA (2007) Culture of HepG2 liver cells on three dimensional polystyrene scaffolds enhances cell structure and function during toxicological challenge. J Anat 211:567-576
4. Gerlach JC, Mutig K, Sauer IM, Schrade P, Efimova E, Mieder T, Naumann G, Grunwald A, Pless G, Mas A, Bachmann S, Neuhaus P, Zeilinger K (2003) Use of primary human liver cells originating from discarded grafts in a bioreactor for liver support therapy and the prospects of culturing adult liver stem cells in bioreactors: a morphologic study. Transplantation 76:781-786
5. Gloeckner H, Jonuleit T, Lemke HD (2001) Monitoring of cell viability and cell growth in a hollow-fiber bioreactor by use of the dye Alamar Blue. J Immunol Methods 252:131-138
6. Hosseinkhani H, Hosseinkhani M, Hattori S, Matsuoka R, Kawaguchi N (2010) Micro and nano-scale in vitro 3D culture system for cardiac stem cells. J Biomed Mater Res A 94:1-8
7. Iwahori T, Matsuno N, Johjima Y, Konno O, Akashi I, Nakamura Y, Hama K, Iwamoto H, Uchiyama M, Ashizawa T, Nagao T (2005) Radial flow bioreactor for the creation of bioartificial liver and kidney. Transplant Proc 37:212-214
8. Lan SF, Starly B (2011) Alginate based 3D hydrogels as an in vitro co-culture model platform for the toxicity screening of new chemical entities. Toxicol Appl Pharmacol 256:62-72
9. Mueller D, Koetemann A, Noor F (2011a) Organotypic cultures of HepG2 cells for in vitro toxicity studies. J Bioeng Biomed Sci S 2:002
10. Mueller D, Tascher G, Muller-Vieira U, Knobeloch D, Nuessler AK, Zeilinger K, Heinzle E, Noor F (2011b) In-depth physiological characterization of primary human hepatocytes in a 3D hollow-fiber bioreactor. J Tissue Eng Regen Med 5:e207-218
11. Mueller D, Muller-Vieira U, Biemel KM, Tascher G, Nussler AK, Noor F (2012) Biotransformation of diclofenac and effects on the metabolome of primary human hepatocytes upon repeated dose exposure. Eur J Pharm Sci 45:716-724
12. Noor F, Niklas J, Muller-Vieira U, Heinzle E (2009) An integrated approach to improved toxicity prediction for the safety assessment during preclinical drug development using Hep G2 cells. Toxicol Appl Pharmacol 237:221-231
13. O'Brien J, Wilson I, Orton T, Pognan F (2000) Investigation of the Alamar Blue (resazurin) fluorescent dye for the assessment of mammalian cell cytotoxicity. Eur J Biochem 267:5421-5426
14. Schmelzer E, Triolo F, Turner ME, Thompson RL, Zeilinger K, Reid LM, Gridelli B, Gerlach JC (2010) Three-Dimensional perfusion bioreactor culture supports differentiation of human fetal liver cells. Tissue Eng Part A 16:2007-2016
15. Schmitmeier S, Langsch A, Jasmund I, Bader A (2006) Development and characterization of a small-scale bioreactor based on a bioartificial hepatic culture model for predictive pharmacological in vitro screenings. Biotechnol Bioeng 95:1198-1206
16. Sodunke TR, Turner KK, Caldwell SA, McBride KW, Reginato MJ, Noh HM (2007) Micropatterns of Matrigel for three-dimensional epithelial cultures. Biomaterials 28:4006-4016
17. Tuschl G, Hrach J, Walter Y, Hewitt PG, Mueller SO (2009) Serum-free collagen sandwich cultures of adult rat hepatocytes maintain liver-like properties long term: a valuable model for in vitro toxicity and drug-drug interaction studies. Chem Biol Interact 181:124-137
18. Zeilinger K, Holland G, Sauer IM, Efimova E, Kardassis D, Obermayer N, Liu M, Neuhaus P, Gerlach JC (2004) Time course of primary liver cell reorganization in three-dimensional high-density bioreactors for extracorporeal liver support: an immunohistochemical and ultrastructural study. Tissue Eng 10:1113-1124