Multicenter comparison of serum and whole-blood specimens for detection of aspergillus DNA in high-risk hematological patients

Journal of Clinical Microbiology

Volumn 51, Issue 5, 2013, Pages 1445-1450

  Springer, Jan ^a,c   Morton, O ^b,e   Perry, Michael ^c   Heinz, Werner J ^a   Paholcsek, Melinda ^a,f   Alzheimer, Mona ^a   Rogers, T R ^b   Barnes, Rosemary A ^d   Einsele, Hermann ^a   Loeffler, Juergen ^a   White, P Lewis ^c  

^a UNIVERSITY HOSPITAL WÜRZBURG   (Germany)

^b ST JAMES S HOSPITAL   (Ireland)

^c PUBLIC HEALTH WALES   (United Kingdom)

^d CARDIFF UNIVERSITY   (United Kingdom)

^e UNIVERSITY OF WESTERN SYDNEY   (Australia)

^f UNIVERSITY OF DEBRECEN   (Hungary)

Author keywords

[No Author keywords available]

Indexed keywords

FUNGAL DNA; GALACTOMANNAN; BIOLOGICAL MARKER; MANNAN;

ADULT; AGED; ARTICLE; ASPERGILLOSIS; ASPERGILLUS; ASPERGILLUS FLAVUS; ASPERGILLUS FUMIGATUS; ASPERGILLUS TERREUS; CANCER PATIENT; CASE CONTROL STUDY; CLINICAL ARTICLE; CONTROLLED STUDY; DIAGNOSTIC TEST ACCURACY STUDY; DISEASE CLASSIFICATION; DISEASE MARKER; FEMALE; HEMATOLOGIC MALIGNANCY; HIGH RISK PATIENT; HUMAN; HUMAN CELL; INTERMETHOD COMPARISON; LABORATORY DEVICE; MALE; MULTICENTER STUDY; NONHUMAN; PERFORMANCE; POLYMERASE CHAIN REACTION; PREDICTIVE VALUE; PRIORITY JOURNAL; PROSPECTIVE STUDY; RETROSPECTIVE STUDY; SENSITIVITY ANALYSIS; SENSITIVITY AND SPECIFICITY; SERUM; ADOLESCENT; BLOOD; CLINICAL TRIAL; ENZYME LINKED IMMUNOSORBENT ASSAY; GENETICS; ISOLATION AND PURIFICATION; MIDDLE AGED; YOUNG ADULT;

ASPERGILLUS;

ADOLESCENT; ADULT; AGED; ASPERGILLOSIS; ASPERGILLUS; BIOLOGICAL MARKERS; CASE-CONTROL STUDIES; DNA, FUNGAL; ENZYME-LINKED IMMUNOSORBENT ASSAY; FEMALE; HUMANS; MALE; MANNANS; MIDDLE AGED; POLYMERASE CHAIN REACTION; RETROSPECTIVE STUDIES; YOUNG ADULT;

EID: 84876762154     PISSN: 00951137     EISSN: 1098660X     Source Type: Journal    
DOI: 10.1128/JCM.03322-12     Document Type: Article

References (32)

1. Denning DW. 1998. Invasive aspergillosis. Clin. Infect. Dis. 26:781-803; quiz 804-805.
2. Meersseman W, Lagrou K, Maertens J, Van Wijngaerden E. 2007. Invasive aspergillosis in the intensive care unit. Clin. Infect. Dis. 45:205-216.
3. Parody R, Martino R, Sánchez F, Subirá M, Hidalgo A, Sierra J. 2009. Predicting survival in adults with invasive aspergillosis during therapy for hematological malignancies or after hematopoietic stem cell transplantation: single-center analysis and validation of the Seattle, French, and Strasbourg prognostic indexes. Am. J. Hematol. 84:571-578.
4. Barnes PD, Marr KA. 2007. Risks, diagnosis and outcomes of invasive fungal infections in haematopoietic stem cell transplant recipients. Br. J. Haematol. 139:519-531.
5. Upton A, Kirby KA, Carpenter P, Boeckh M, Marr KA. 2007. Invasive aspergillosis following hematopoietic cell transplantation: outcomes and prognostic factors associated with mortality. Clin. Infect. Dis. 44:531-540.
6. White PL, Linton CJ, Perry MD, Johnson EM, Barnes RA. 2006. The evolution and evaluation of a whole blood polymerase chain reaction assay for the detection of invasive aspergillosis in hematology patients in a routine clinical setting. Clin. Infect. Dis. 42:479-486.
7. Challier S, Boyer S, Abachin E, Berche P. 2004. Development of a serum-based Taqman real-time PCR assay for diagnosis of invasive aspergillosis. J. Clin. Microbiol. 42:844-846.
8. Loeffler J, Henke N, Hebart H, Schmidt D, Hagmeyer L, Schumacher U, Einsele H. 2000. Quantification of fungal DNA by using fluorescence resonance energy transfer and the light cycler system. J. Clin. Microbiol. 38:586-590.
9. White PL, Bretagne S, Klingspor L, Melchers WJ, McCulloch E, Schulz B, Finnstrom N, Mengoli C, Barnes RA, Donnelly JP, Loeffler J, European Aspergillus PCR Initiative. 2010. Aspergillus PCR: one step closer to standardization. J. Clin. Microbiol. 48:1231-1240.
10. White PL, Mengoli C, Bretagne S, Cuenca-Estrella M, Finnstrom N, Klingspor L, Melchers WJ, McCulloch E, Barnes RA, Donnelly JP, Loeffler J, European Aspergillus PCR Initiative (EAPCRI). 2011. Evaluation of Aspergillus PCR protocols for testing serum specimens. J. Clin. Microbiol. 49:3842-3848.
11. White PL, Perry MD, Loeffler J, Melchers W, Klingspor L, Bretagne S, McCulloch E, Cuenca-Estrella M, Finnstrom N, Donnelly JP, Barnes RA, European Aspergillus PCR Initiative. 2010. Critical stages of extracting DNA from Aspergillus fumigatus in whole-blood specimens. J. Clin. Microbiol. 48:3753-3755.
12. Morton CO, Loeffler J, De Luca A, Frost S, Kenny C, Duval S, Romani L, Rogers TR. 2010. Dynamics of extracellular release of Aspergillus fumigatus DNA and galactomannan during growth in blood and serum. J. Med. Microbiol. 59:408-413.
13. White PL, Perry MD, Barnes RA. 2009. An update on the molecular diagnosis of invasive fungal disease. FEMS Microbiol. Lett. 296:1-10.
14. De Pauw B, Walsh TJ, Donnelly JP, Stevens DA, Edwards JE, Calandra T, Pappas PG, Maertens J, Lortholary O, Kauffman CA, Denning DW, Patterson TF, Maschmeyer G, Bille J, Dismukes WE, Herbrecht R, Hope WW, Kibbler CC, Kullberg BJ, Marr KA, Muñoz P, Odds FC, Perfect JR, Restrepo A, Ruhnke M, Segal BH, Sobel JD, Sorrell TC, Viscoli C, Wingard JR, Zaoutis T, Bennett JE, European Organization for Research and Treatment of Cancer/Invasive Fungal Infections Cooperative Group, National Institute of Allergy and Infectious Diseases Mycoses Study Group (EORTC/MSG) Consensus Group. 2008. Revised definitions of invasive fungal disease from the European Organization for Research and Treatment of Cancer/Invasive Fungal Infections Cooperative Group and the National Institute of Allergy and Infectious Diseases Mycoses Study Group (EORTC/MSG) Consensus Group. Clin. Infect. Dis. 46:1813-1821.
15. Rogers TR, Morton CO, Springer J, Conneally E, Heinz W, Kenny C, Frost S, Einsele H, Loeffler J. Combined real-time PCR and galactomannan surveillance improves diagnosis of invasive aspergillosis in high risk patients with haematological malignancies. Br. J. Haematol., in press.
16. Springer J, Loeffler J, Heinz W, Schlossnagel H, Lehmann M, Morton O, Rogers TR, Schmitt C, Frosch M, Einsele H, Kurzai O. 2011. Pathogen-specific DNA enrichment does not increase sensitivity of PCR for diagnosis of invasive aspergillosis in neutropenic patients. J. Clin. Microbiol. 49:1267-1273.
17. White PL, Perry MD, Moody A, Follett SA, Morgan G, Barnes RA. 2011. Evaluation of analytical and preliminary clinical performance of Myconostica MycAssay Aspergillus when testing serum specimens for diagnosis of invasive aspergillosis. J. Clin. Microbiol. 49:2169-2174.
18. Springer J, Schlossnagel H, Heinz W, Doedt T, Soeller R, Einsele H, Loeffler J. 2012. A novel extraction method combining plasma with whole-blood fraction shows excellent sensitivity and reproducibility for patients at high risk for invasive aspergillosis. J. Clin. Microbiol. 50:2585-2891.
19. Morton CO, Clemons KV, Springer J, Mueller JG, Rogers TR, Stevens DA, Kurzai O, Einsele H, Loeffler J. 2011. Real-time PCR and quantitative culture for monitoring of experimental Aspergillus fumigatus intracranial infection in neutropenic mice. J. Med. Microbiol. 60:913-919.
20. Armitage P, Berry G, Matthews JNS. 2002. Statistical methods in medical research, 4th ed. Blackwell Science, Ltd., Oxford, United Kingdom.
21. Newcombe RG. 1998. Improved confidence intervals for the difference between binomial proportions based on paired data. Stat. Med. 17:2635-2650.
22. Newcombe RG. 1998. Interval estimation for the difference between independent proportions: comparison of eleven methods. Stat. Med. 17: 873-890.
23. Mengoli C, Cruciani M, Barnes RA, Loeffler J, Donnelly JP. 2009. Use of PCR for diagnosis of invasive aspergillosis: systematic review and metaanalysis. Lancet Infect. Dis. 9:89-96.
24. Botterel F, Farrugia C, Ichai P, Costa JM, Saliba F, Bretagne S. 2008. Real-time PCR on the first galactomannan-positive serum sample for diagnosing invasive aspergillosis in liver transplant recipients. Transpl. Infect. Dis. 10:333-338.
25. Millon L, Piarroux R, Deconinck E, Bulabois CE, Grenouillet F, Rohrlich P, Costa JM, Bretagne S. 2005. Use of real-time PCR to process the first galactomannan-positive serum sample in diagnosing invasive aspergillosis. J. Clin. Microbiol. 43:5097-5101.
26. Cuenca-Estrella M, Meije Y, Diaz-Pedroche C, Gomez-Lopez A, Buitrago MJ, Bernal-Martinez L, Grande C, Juan RS, Lizasoain M, Rodriguez-Tudela JL, Aguado JM. 2009. Value of serial quantification of fungal DNA by a real-time PCR-based technique for early diagnosis of invasive aspergillosis in patients with febrile neutropenia. J. Clin. Microbiol. 47: 379-384.
27. Lass-Flörl C, Aigner J, Gunsilius E, Petzer A, Nachbaur D, Gastl G, Einsele H, Loffler J, Dierich MP, Würzner R. 2001. Screening for Aspergillus spp. using polymerase chain reaction of whole blood samples from patients with haematological malignancies. Br. J. Haematol. 113:180-184.
28. McCulloch E, Ramage G, Rajendran R, Lappin DF, Jones B, Warn P, Shrief R, Kirkpatrick WR, Patterson TF, Williams C. 2012. Antifungal treatment affects the laboratory diagnosis of invasive aspergillosis. J. Clin. Pathol. 65:83-86.
29. Loeffler J, Hebart H, Brauchle U, Schumacher U, Einsele H. 2000. Comparison between plasma and whole blood specimens for detection of Aspergillus DNA by PCR. J. Clin. Microbiol. 38:3830-3833.
30. Costa C, Vidaud D, Olivi M, Bart-Delabesse E, Vidaud M, Bretagne S. 2001. Development of two real-time quantitative TaqMan PCR assays to detect circulating Aspergillus fumigatus DNA in serum. J. Microbiol. Methods 44:263-269.
31. Bernal-Martinez L, Gago S, Buitrago MJ, Gomez-Lopez A, Rodriguez-Tudela JL, Cuenca-Estrella M. 2011. Analysis of performance of a PCRbased assay to detect DNA of Aspergillus fumigatus in whole blood and serum: a comparative study with clinical samples. J. Clin. Microbiol. 49: 3596-3599.
32. Meije Y, Aguado JM, Cuenca-Estrella M. 2011. Silent and prolonged Aspergillus DNAemia in oncohematological patients receiving antifungal prophylaxis: a new phenomenon with clinical implications. Bone Marrow Transplant. 46:1016-1017.