Compensation of the enantioselectivity-activity trade-off in the directed evolution of an esterase from Rhodobacter sphaeroides by site-directed saturation mutagenesis

Applied Microbiology and Biotechnology

Volumn 97, Issue 8, 2013, Pages 3355-3362

  Guo, Fei ^a   Xu, Haoming ^a   Xu, Hongna ^a   Yu, Hongwei ^a  

^a ZHEJIANG UNIVERSITY   (China)

Author keywords

Directed evolution; Enantioselectivity activity trade off; Kinetic resolution; Molecular dynamics simulation; Site directed saturation mutagenesis

Indexed keywords

DIRECTED EVOLUTION; ENANTIOSELECTIVITY-ACTIVITY TRADE-OFF; KINETIC RESOLUTION; MOLECULAR DYNAMICS SIMULATIONS; SATURATION MUTAGENESIS;

ENZYMES; ESTERS; ISOMERS; MOLECULAR DYNAMICS; MUTAGENESIS;

ENANTIOSELECTIVITY;

ESTERASE; TYROSINE;

BACTERIUM; ENZYME ACTIVITY; MOLECULAR ANALYSIS; MUTAGENICITY; MUTATION; NUMERICAL MODEL; REACTION KINETICS; SPATIAL RESOLUTION; TRADE-OFF;

ARTICLE; ENANTIOSELECTIVITY; ENZYME ACTIVITY; HIGH THROUGHPUT SCREENING; KINETICS; MICHAELIS CONSTANT; MOLECULAR DYNAMICS; MUTAGENESIS; MUTANT; NONHUMAN; RHODOBACTER SPHAEROIDES; WILD TYPE;

DIRECTED MOLECULAR EVOLUTION; DNA MUTATIONAL ANALYSIS; ESTERASES; KINETICS; MANDELIC ACIDS; MOLECULAR DYNAMICS SIMULATION; MUTAGENESIS, SITE-DIRECTED; MUTANT PROTEINS; RHODOBACTER SPHAEROIDES; SUBSTRATE SPECIFICITY;

RHODOBACTER SPHAEROIDES;

EID: 84876679487     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-012-4516-z     Document Type: Article

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