Pseudomonas aeruginosa

PCR for Clinical Microbiology: An Australian and International Perspective

Volumn , Issue , 2010, Pages 191-195

  Anuj, Snehal ^a   Whiley, David M ^a  

^a Queensland Children's Medical Research Institute   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 84876677524     PISSN: None     EISSN: None     Source Type: Book    
DOI: 10.1007/978-90-481-9039-3_24     Document Type: Chapter

References (12)

1. Anuj SN, Whiley DM, Kidd TJ et al (2009) Identification of Pseudomonas aeruginosa by a duplex real-time polymerase chain reaction assay targeting the ecf X and the gyrB genes. Diagn Microbiol Infect Dis 63:127-131
2. Hoiby N, Frederiksen B, Pressler T (2005) Eradication of early Pseudomonas aeruginosa infection. J Cyst Fibros 4:49-54
3. Jaffe RI, Lane JD, Bates CW (2001) Real-time identification of Pseudomonas aeruginosa direct from clinical samples using a rapid extraction method and polymerase chain reaction (PCR). J Clin Lab Anal 15:131-137
4. Khan AA, Cerniglia CE (1994) Detection of Pseudomonas aeruginosa from clinical and environmental samples by amplification of the exotoxin A gene using PCR. Appl Environ Microbiol 60:3739-3745
5. Lavenir R, Jocktane D, Laurent F et al (2007) Improved reliability of Pseudomonas aeruginosa PCR detection by the use of the species-specific ecf X gene target. J Microbiol Methods 70:20-29
6. Motoshima M, Yanagihara K, Fukushima K et al (2007) Rapid and accurate detection of Pseudomonas aeruginosa by real-time polymerase chain reaction with melting curve analysis targeting gyrB gene. Diagn Microbiol Infect Dis 58:53-58
7. Qin X, Emerson J, Stapp J et al (2003) Use of real-time PCR with multiple targets to identify Pseudomonas aeruginosa and other non-fermenting gram-negative bacilli from patients with cystic fibrosis. J Clin Microbiol 41:4312-4317
8. Spilker T, Coenye T, Vandamme P et al (2004) PCR-based assay for differentiation of Pseudomonas aeruginosa from other pseudomonas species recovered from cystic fibrosis patients. J Clin Microbiol 42:2074-2079
9. Syrmis MW, O'Carroll MR, Sloots TP et al (2004) Rapid genotyping of Pseudomonas aeruginosa isolates harboured by adult and paediatric patients with cystic fibrosis using repetitive-element-based PCR assays. J Med Microbiol 53:1089-1096
10. Tummler B, Kiewitz C (1999) Cystic fibrosis: an inherited susceptibility to bacterial respiratory infections. Mol Med Today 5:351-358
11. Wellinghausen N, Kothe J, Wirths B et al (2005) Superiority of molecular techniques for identification of Gram-negative, oxidasepositive rods, including morphologically nontypical Pseudomonas aeruginosa, from patients with cystic fibrosis. J Clin Microbiol 43:4070-4075
12. Whiley DM, Lambert SB, Bialasiewicz S et al (2008) False-negative results in nucleic acid amplification tests: do we need to routinely use two genetic targets in all assays to overcome problems caused by sequence variation? Crit Rev Microbiol 34:71-76