Superiority of molecular techniques for identification of gram-negative, oxidase-positive rods, including morphologically nontypical Pseudomonas aeruginosa, from patients with cystic fibrosis

Journal of Clinical Microbiology

Volumn 43, Issue 8, 2005, Pages 4070-4075

  Wellinghausen, Nele ^a   Köthe, Juliane ^a   Wirths, Beate ^a   Sigge, Anja ^a   Poppert, Sven ^a  

^a UNIVERSITY OF ULM   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

OXIDOREDUCTASE; RNA 16S;

ARTICLE; BACTERIUM IDENTIFICATION; BACTERIUM ISOLATE; COMPARATIVE STUDY; CONTROLLED STUDY; CYSTIC FIBROSIS; DIAGNOSTIC PROCEDURE; FLUORESCENCE IN SITU HYBRIDIZATION; GENE SEQUENCE; GRAM NEGATIVE BACTERIUM; NONHUMAN; NUCLEOTIDE SEQUENCE; PHENOTYPE; PRIORITY JOURNAL; PSEUDOMONAS AERUGINOSA; REAL TIME POLYMERASE CHAIN REACTION; RECA GENE; RISK ASSESSMENT; RNA GENE; VALIDATION PROCESS;

CYSTIC FIBROSIS; GRAM-NEGATIVE BACTERIA; HUMANS; IN SITU HYBRIDIZATION, FLUORESCENCE; OXIDOREDUCTASES; POLYMERASE CHAIN REACTION; PSEUDOMONAS AERUGINOSA; REAGENT STRIPS; RNA, RIBOSOMAL, 16S; SEQUENCE ANALYSIS, DNA;

NEGIBACTERIA; PSEUDOMONAS AERUGINOSA;

EID: 23744443828     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/JCM.43.8.4070-4075.2005     Document Type: Article

References (24)

1. Amann, R. I., B. J. Binder, R. J. Olson, S. W. Chisholm, R. Devereux, and D. A. Stahl. 1990. Combination of 16S rRNA-targeted oligonucleotide probes with flew cytometry for analyzing mixed microbial populations. Appl. Environ. Microbiol. 56:1919-1925.
2. Coenye, T., J. Goris, T. Spilker, P. Vandamme, and J. J. LiPuma. 2002. Characterization of unusual bacteria isolated from respiratory secretions of cystic fibrosis patients and description of Inquilinus limosus gen. nov., sp. nov. J. Clin. Microbiol. 40:2062-2069.
3. Coenye, T., L. Liu, P. Vandamme, and J. J. LiPuma. 2001. Identification of Pandoraea species by 16S ribosomal DNA-based PCR assays. J. Clin. Microbiol. 39:4452-4455.
4. Coenye, T., P. Vandamme, and J. J. LiPuma. 2002. Infection by Ralstonia species in cystic fibrosis patients: identification of R. pickeitii and R. mannitolilytica by polymerase chain reaction. Emerg. Infect. Dis. 8:692-696.
5. Coenye, T., P. Vandamme, and J. J. LiPuma. 2003. Ralstonia respiraculi sp. nov., isolated from the respiratory tract of cystic fibrosis patients. Int. J. Syst. Evol. Microbiol. 53:1339-1342.
6. Ferroni, A., I. Sermet-Gaudelus, E. Abachin, G. Quesne, G. Lenoir, P. Berche, and J. L. Gaillard. 2002. Use of 16S rRNA gene sequencing for identification of nonfermenting gram-negative bacilli recovered from patients attending a single cystic fibrosis center. J. Clin. Microbiol. 40:3793-3797.
7. Hiraishi, A. 1992. Direct automated sequencing of 16S rDNA amplified by polymerase chain reaction from bacterial cultures without DNA purification. Lett. Appl. Microbiol. 15:210-213.
8. Hogardt, M., K. Trebesius, A. M. Geiger, M. Hornef, J. Rosenecker, and J. Heesemann. 2000. Specific and rapid detection by fluorescent in situ hybridization of bacteria in clinical samples obtained from cystic fibrosis patients. J. Clin. Microbiol. 38:818-825.
9. Karpati, F., and J. Jonasson. 1996. Polymerase chain reaction for the detection of Pseudomonas aeruginosa, Stenotrophomonas maltophilia and Burkholderia cepacia in sputum of patients with cystic fibrosis. Mol. Cell Probes 10:397-403.
10. Kempf, V. A., K. Trebesius, and I. B. Autenrieth. 2000. Fluorescent in situ hybridization allows rapid identification of microorganisms in blood cultures. J. Clin. Microbiol. 38:830-838.
11. Kiska, D. L., and P. H. Gilligan. 2003. Pseudomonas, p. 719-728. In P. R. Murray, E. J. Baron, M. A. Pfaller, F. C. Tenover, and R. H. Yolken (ed.), Manual of clinical microbiology, 8th ed. American Society for Microbiology, Washington, D.C.
12. Kiska, D. L., A. Kerr, M. C. Jones, J. A. Caracciolo, B. Eskridge, M. Jordan, S. Miller, D. Hughes, N. King, and P. H. Gilligan. 1996. Accuracy of four commercial systems for identification of Burkholderia cepacia and other gram-negative nonfermenting bacilli recovered from patients with cystic fibrosis. J. Clin. Microbiol. 34:886-891.
13. Klinger, J. D., and M. J. Thomassen. 1985. Occurrence and antimicrobial susceptibility of gram-negative nonfermentative bacilli in cystic fibrosis patients. Diagn. Microbiol. Infect. Dis. 3:149-158.
14. Lyczak, J. B., C. L. Cannon, and G. B. Pier. 2002. Lung infections associated with cystic fibrosis. Clin. Microbiol. Rev. 15:194-222.
15. Mahenthiralingam, E., J. Bischof, S. K. Byrne, C. Radomski, J. E. Davies, Y. Av-Gay, and P. Vandamme. 2000. DNA-based diagnostic approaches for identification of Burkholderia cepacia complex, Burkholderia vietnamiensis, Burkholderia multivorans, Burkholderia stabilis, and Burkholderia cepacia genomovars I and III. J. Clin. Microbiol. 38:3165-3173.
16. McMenamin, J. D., T. M. Zaccone, T. Coenye, P. Vandamme, and J. J. LiPuma. 2000. Misidentification of Burkholderia cepacia in US cystic fibrosis treatment centers: an analysis of 1,051 recent sputum isolates. Chest 117:1661-1665.
17. Miller, M. B., and P. H. Gilligan. 2003. Laboratory aspects of management of chronic pulmonary infections in patients with cystic fibrosis. J. Clin. Microbiol. 41:4009-4015.
18. Qin, X., J. Emerson, J. Stapp, L. Stapp, P. Abe, and J. L. Burns. 2003. Use of real-time PCR with multiple targets to identify Pseudomonas aeruginosa and other nonfermenting gram-negative bacilli from patients with cystic fibrosis. J. Clin. Microbiol. 41:4312-4317.
19. Spilker, T., T. Coenye, P. Vandamme, and J. J. LiPuma. 2004. PCR-based assay for differentiation of Pseudomonas aeruginosa from other Pseudomonas species recovered from cystic fibrosis patients. J. Clin. Microbiol. 42:2074-2079.
20. Tenover, F. C., R. D. Arbeit, R. V. Goering, P. A. Mickelsen, B. E. Murray, D. H. Persing, and B. Swaminathan. 1995. Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing. J. Clin. Microbiol. 33:2233-2239.
21. van Pelt, C., C. M. Verduin, W. H. Goessens, M. C. Vos, B. Tummler, C. Segonds, F. Reubsaet, H. Verbrugh, and A. van Belkum. 1999. Identification of Burkholderia spp. in the clinical microbiology laboratory: comparison of conventional and molecular methods. J. Clin. Microbiol. 37:2158-2164.
22. Wallet, F., T. Perez, S. Armand, B. Wallaert, and R. J. Courcol. 2002. Pneumonia due to Bordetella bronchiseptica in a cystic fibrosis patient: 16S rRNA sequencing for diagnosis confirmation. J. Clin. Microbiol. 40:2300-2301.
23. Wellinghausen, N., A. Essig, and O. Sommerburg. 2005. Inquilinus limosus in patients with Cystic Fibrosis, Germany. Emerg. Infect. Dis. 11:457-459.
24. Wellinghausen, N., B. Wirths, A. R. Franz, L. Karolyi, R. Marre, and U. Reischl. 2004. Algorithm for the identification of bacterial pathogens in positive blood cultures by real-time LightCycler polymerase chain reaction (PCR) with sequence-specific probes. Diagn. Microbiol. Infect. Dis. 48:229-241.