DNase inhibits gardnerella vaginalis biofilms in vitro and in vivo

Journal of Infectious Diseases

Volumn 207, Issue 10, 2013, Pages 1491-1497

  Hymes, Saul R ^a   Randis, Tara M ^a   Sun, Thomas Yang ^a   Ratner, Adam J ^a  

^a Och Spine at New York Presbyterian Hospitals   (United States)

Author keywords

Bacterial vaginosis; biofilm; extracellular DNA; Gardnerella vaginalis

Indexed keywords

DEOXYRIBONUCLEASE; DEOXYRIBONUCLEASE I; DNA; EXTRACELLULAR DNA; METRONIDAZOLE; STREPTOMYCIN; UNCLASSIFIED DRUG;

ANIMAL EXPERIMENT; ANIMAL MODEL; ANTIBACTERIAL ACTIVITY; ANTIBIOTIC SENSITIVITY; ARTICLE; BACTERIAL COLONIZATION; BIOFILM; CONCENTRATION RESPONSE; CONTROLLED STUDY; DNA DAMAGE; DRUG POTENTIATION; ENZYME ACTIVITY; FEMALE; GARDNERELLA VAGINALIS; IN VITRO STUDY; IN VIVO STUDY; INFECTION RISK; MICROBIAL MORPHOLOGY; MORBIDITY; MOUSE; NONHUMAN; PRIORITY JOURNAL; RECURRENCE RISK; RISK REDUCTION; SUPERNATANT; VAGINITIS;

ANIMALS; ANTI-INFECTIVE AGENTS; BIOFILMS; DEOXYRIBONUCLEASES; DISEASE MODELS, ANIMAL; FEMALE; GARDNERELLA VAGINALIS; METAGENOME; METRONIDAZOLE; MICE; MICE, INBRED C57BL; VAGINA; VAGINOSIS, BACTERIAL;

EID: 84876537270     PISSN: 00221899     EISSN: None     Source Type: Journal    
DOI: 10.1093/infdis/jit047     Document Type: Article

References (29)

1. Schwebke JR. Gynecologic consequences of bacterial vaginosis. Obstet Gynecol Clin N Am 2003; 30:685-94. (Pubitemid 38018632)
2. Atashili J, Poole C, Ndumbe PM, Adimora AA, Smith JS. Bacterial vaginosis and HIV acquisition: a meta-analysis of published studies. AIDS 2008; 22:1493-501.
3. Brotman RM, Klebanoff MA, Nansel TR, et al. Bacterial vaginosis assessed by gram stain and diminished colonization resistance to incident gonococcal, chlamydial, and trichomonal genital infection. J Infect Dis 2010; 202:1907-15.
4. Hillier SL, Martius J, Krohn M, Kiviat N, Holmes KK, Eschenbach DA. A case-control study of chorioamnionic infection and histologic chorioamnionitis in prematurity. N Engl J Med 1988; 319:972-8.
5. Marrazzo JM, Martin DH, Watts DH, et al. Bacterial vaginosis: identifying research gaps proceedings of a workshop sponsored by DHHS/NIH/NIAID. Sex Transm Dis 2010; 37:732-44.
6. Josey WE, Schwebke JR. The polymicrobial hypothesis of bacterial vaginosis causation: a reassessment. Int J STD AIDS 2008; 19:152-4. (Pubitemid 351738788)
7. Fredricks DN, Fiedler TL, Marrazzo JM. Molecular identification of bacteria associated with bacterial vaginosis. N Engl J Med 2005; 353: 1899-911. (Pubitemid 41565955)
8. Sha BE, Chen HY, Wang QJ, Zariffard MR, Cohen MH, Spear GT. Utility of Amsel criteria, Nugent score, and quantitative PCR for Gardnerella vaginalis, Mycoplasma hominis, and Lactobacillus spp. for diagnosis of bacterial vaginosis in human immunodeficiency virus-infected women. J Clin Microbiol 2005; 43:4607-12. (Pubitemid 41298250)
9. Gelber SE, Aguilar JL, Lewis KLT, Ratner AJ. Functional and phylogenetic characterization of vaginolysin, the human-specific cytolysin from Gardnerella vaginalis. J Bacteriol 2008; 190:3896-903. (Pubitemid 351732868)
10. Patterson JL, Girerd PH, Karjane NW, Jefferson KK. Effect of biofilm phenotype on resistance of Gardnerella vaginalis to hydrogen peroxide and lactic acid. Am J Obstet Gynecol 2007; 197:170.e1-7.
11. Patterson JL, Stull-Lane A, Girerd PH, Jefferson KK. Analysis of adherence, biofilm formation and cytotoxicity suggests a greater virulence potential of Gardnerella vaginalis relative to other bacterialvaginosis- associated anaerobes. Microbiology 2010; 156:392-9.
12. Swidsinski A, Mendling W, Loening-Baucke V, et al. Adherent biofilms in bacterial vaginosis. Obstet Gynecol 2005; 106:1013-23. (Pubitemid 41532908)
13. Swidsinski A, Mendling W, Loening-Baucke V, et al. An adherent Gardnerella vaginalis biofilm persists on the vaginal epithelium after standard therapy with oral metronidazole. Am J Obstet Gynecol 2008; 198:97.e1-6.
14. Swidsinski A, Doerffel Y, Loening-Baucke V, et al. Gardnerella biofilm involves females and males and is transmitted sexually. Gynecol Obstet Invest 2010; 70:256-63.
15. Steichen CT, Cho C, Shao JQ, Apicella MA. The Neisseria gonorrhoeae biofilm matrix contains DNA, and an endogenous nuclease controls its incorporation. Infect Immun 2011; 79:1504-11.
16. Whitchurch CB, Tolker-Nielsen T, Ragas PC, Mattick JS. Extracellular DNA required for bacterial biofilm formation. Science 2002; 295:1487. (Pubitemid 34173996)
17. Mann EE, Rice KC, Boles BR, et al. Modulation of eDNA release and degradation affects Staphylococcus aureus biofilm maturation. PLoS One 2009; 4:e5822.
18. Randis TM, Kulkarni R, Aguilar JL, Ratner AJ. Antibody-based detection and inhibition of vaginolysin, the Gardnerella vaginalis cytolysin. PLoS One 2009; 4:e5207.
19. Jerse AE, Wu H, Packiam M, Vonck RA, Begum AA, Garvin LE. Estradiol-treated female mice as surrogate hosts for Neisseria gonorrhoeae genital tract infections. Front Microbiol 2011; 2:107.
20. Stewart PS, Costerton JW. Antibiotic resistance of bacteria in biofilms. Lancet 2001; 358:135-8. (Pubitemid 32709857)
21. Tetz GV, Artemenko NK, Tetz VV. Effect of DNase and antibiotics on biofilm characteristics. Antimicrob Agents Chemother 2009; 53: 1204-9.
22. Kaplan JB, Lovetri K, Cardona ST, et al. Recombinant human DNase I decreases biofilm and increases antimicrobial susceptibility in staphylococci. J Antibiot 2012; 65:73-7.
23. Eckert LO. Clinical practice. Acute vulvovaginitis. N Engl J Med 2006; 355:1244-52.
24. McDonald HM, Brocklehurst P, Gordon A. Antibiotics for treating bacterial vaginosis in pregnancy. Cochrane Database Syst Rev 2007; CD000262. http://www.ncbi.nlm.nih.gov/pubmed/17253447.
25. Guiton PS, Hung CS, Kline KA, et al. Contribution of autolysin and sortase A during Enterococcus faecalis DNA-dependent biofilm development. Infect Immun 2009; 77:3626-38.
26. Liu Y, Burne RA. The major autolysin of Streptococcus gordonii is subject to complex regulation and modulates stress tolerance, biofilm formation, and extracellular-DNA release. J Bacteriol 2011; 193:2826-37.
27. Heilmann C, Hartleib J, Hussain MS, Peters G. The multifunctional Staphylococcus aureus autolysin Aaa mediates adherence to immobilized fibrinogen and fibronectin. Infect Immun 2005; 73: 4793-802. (Pubitemid 41105635)
28. Eisenberg JD, Aitken ML, Dorkin HL, et al. Safety of repeated intermittent courses of aerosolized recombinant human deoxyribonuclease in patients with cystic fibrosis. J Pediatr 1997; 131:118-24. (Pubitemid 27498319)
29. Rahman NM, Maskell NA, West A, et al. Intrapleural use of tissue plasminogen activator and DNase in pleural infection. N Engl J Med 2011; 365:518-26.