The Neisseria gonorrhoeae biofilm matrix contains DNA, and an endogenous nuclease controls its incorporation

Infection and Immunity

Volumn 79, Issue 4, 2011, Pages 1504-1511

  Steichen, Christopher T ^a   Cho, Christine ^a   Shao, Jian Q ^a   Apicella, Michael A ^a  

^a UNIVERSITY OF IOWA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL DNA; NUCLEASE;

ARTICLE; BACTERIAL GENE; BACTERIAL STRAIN; BIOFILM; ENZYME ACTIVITY; GENE IDENTIFICATION; GENE SEQUENCE; NEISSERIA GONORRHOEAE; NONHUMAN; NUC GENE; PHENOTYPE; PRIORITY JOURNAL; SEQUENCE ANALYSIS; SEQUENCE HOMOLOGY;

AMINO ACID SEQUENCE; BACTERIAL PROTEINS; BIOFILMS; DNA, BACTERIAL; GENES, BACTERIAL; MICROCOCCAL NUCLEASE; MICROSCOPY, CONFOCAL; MOLECULAR SEQUENCE DATA; NEISSERIA GONORRHOEAE; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION;

EID: 79953308094     PISSN: 00199567     EISSN: 10985522     Source Type: Journal    
DOI: 10.1128/IAI.01162-10     Document Type: Article

References (23)

1. Allesen-Holm, M., et al. 2006. A characterization of DNA release in Pseudomonas aeruginosa cultures and biofilms. Mol. Microbiol. 59:1114-1128.
2. Apicella, M. A. 1974. Antigenically distinct populations of Neisseria gonorrhoeae: isolation and characterization of the responsible determinants. J. Infect. Dis. 130:619-625.
3. Bauer, H. M., and M. K. M. Samuel. 2005. Prevalence of and associated risk factors for fluoroquinolone-resistant Neisseria gonorrhoeae in California. Clin. Infect. Dis. 41:795-803.
4. Boles, B. R., and P. K. Singh. 2008. Endogenous oxidative stress produces diversity and adaptability in biofilm communities. Proc. Natl. Acad. Sci. U. S. A. 105:12503-12508.
5. Boles, B. R., M. Thoendel, A. J. Roth, and A. R. Horswill. 2010. Identification of genes involved in polysaccharide-independent Staphylococcus aureus biofilm formation. PLoS One 5:e10146.
6. Branda, S. S., S. Vik, L. Friedman, and R. Kolter. 2005. Biofilms: the matrix revisited. Trends Microbiol. 13:20-26.
7. Cannon, J. G., W. J. Black, I. Nachamkin, and P. W. Stewart. 1984. Monoclonal antibody that recognizes an outer membrane antigen common to the pathogenic Neisseria species but not to most nonpathogenic Neisseria species. Infect. Immun. 43:994-999.
8. Dorward, D. W., C. F. Garon, and R. C. Judd. 1989. Export and intercellular transfer of DNA via membrane blebs of Neisseria gonorrhoeae. J. Bacteriol. 171:2499-2505.
9. Falsetta, M. L., et al. 2009. Transcriptional profiling identifies the metabolic phenotype of gonococcal biofilms. Infect. Immun. 77:3522-3532.
10. Falsetta, M. L., A. G. McEwan, M. P. Jennings, and M. A. Apicella. 2010. Anaerobic metabolism occurs in the substratum of gonococcal biofilms and may be sustained in part by nitric oxide. Infect. Immun. 78:2320-2328.
11. Ferretti, J. J., D. W. Dyer, and B. A. Roe. 1997. Data available. Nature 386:320.
12. Greiner, L. L., et al. 2005. Biofilm formation by Neisseria gonorrhoeae. Infect. Immun. 73:1964-1970.
13. Gunn, J. S., A. Piekarowicz, R. Chien, and D. C. Stein. 1992. Cloning and linkage analysis of Neisseria gonorrhoeae DNA methyltransferases. J. Bacteriol. 174:5654-5660.
14. Heydorn, A., et al. 2000. Quantification of biofilm structures by the novel computer program COMSTAT. Microbiology 146(Part 10):2395-2407.
15. Hoiby, N., O. Ciofu, and T. Bjarnsholt. 2010. Pseudomonas aeruginosa biofilms in cystic fibrosis. Future Microbiol. 5:1663-1674.
16. Hook, E., and H. Handsfield. 1999. Gonococcal infections in adults, 3rd ed. McGraw-Hill, New York, NY.
17. Jurcisek, J. A., and L. O. Bakaletz. 2007. Biofilms formed by nontypeable Haemophilus influenzae in vivo contain both double-stranded DNA and type IV pilin protein. J. Bacteriol. 189:3868-3875.
18. Mah, T. F., and G. A. O'Toole. 2001. Mechanisms of biofilm resistance to antimicrobial agents. Trends Microbiol. 9:34-39.
19. Matsukawa, M., and E. P. Greenberg. 2004. Putative exopolysaccharide synthesis genes influence Pseudomonas aeruginosa biofilm development. J. Bacteriol. 186:4449-4456.
20. Rice, K. C., et al. 2007. The cidA murein hydrolase regulator contributes to DNA release and biofilm development in Staphylococcus aureus. Proc. Natl. Acad. Sci. U. S. A. 104:8113-8118.
21. Steichen, C. T., J. Q. Shao, M. R. Ketterer, and M. A. Apicella. 2008. Gonococcal cervicitis: a role for biofilm in pathogenesis. J. Infect. Dis. 198:1856-1861.
22. van Schaik, E. J., et al. 2005. DNA binding: a novel function of Pseudomonas aeruginosa type IV pili. J. Bacteriol. 187:1455-1464.
23. Whitchurch, C. B., T. Tolker-Nielsen, P. C. Ragas, and J. S. Mattick. 2002. Extracellular DNA required for bacterial biofilm formation. Science 295: 1487.