Effective Identification of Akt Interacting Proteins by Two-Step Chemical Crosslinking, Co-Immunoprecipitation and Mass Spectrometry

PLoS ONE

Volumn 8, Issue 4, 2013, Pages

  Huang, Bill X ^a   Kim, Hee Yong ^a  

^a NATIONAL INSTITUTE ON ALCOHOL ABUSE AND ALCOHOLISM   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CHEMICAL COMPOUND; DITHIOBIS[SUCCINIMIDYLPROPIONATE]; ENZYME ANTIBODY; PROTEIN KINASE B; PROTEIN KINASE B ANTIBODY; SUBERIC ACID BIS(N HYDROXYSUCCINIMIDE) ESTER; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; CONTROLLED STUDY; CROSS LINKING; ENZYME BINDING; ENZYME STABILITY; IMMUNOPRECIPITATION; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; MOLECULAR PROBE; MOUSE; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN ANALYSIS; PROTEIN IMMOBILIZATION; PROTEIN PROTEIN INTERACTION; SENSITIVITY ANALYSIS; TANDEM MASS SPECTROMETRY;

AMINO ACID SEQUENCE; ANIMALS; BLOTTING, WESTERN; CELL LINE, TUMOR; CROSS-LINKING REAGENTS; ENZYME ACTIVATION; EXTRACELLULAR SIGNAL-REGULATED MAP KINASES; IMMUNOPRECIPITATION; MICE; MOLECULAR SEQUENCE DATA; PEPTIDES; PROTEIN BINDING; PROTO-ONCOGENE PROTEINS C-AKT; SOMATOMEDINS; TANDEM MASS SPECTROMETRY;

EID: 84876220602     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0061430     Document Type: Article

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