A real-time PCR assay for detection and quantification of Verticillium dahliae in spinach seed

Phytopathology

Volumn 102, Issue 4, 2012, Pages 443-451

  Duressa, Dechassa ^a   Rauscher, Gilda ^a,d   Koike, Steven T ^b   Mou, Beiquan ^a   Hayes, Ryan J ^a   Maruthachalam, Karunakaran ^c   Subbarao, Krishna V ^c   Klosterman, Steven J ^a  

^a AGRICULTURAL RESEARCH SERVICE   (United States)

^b UNIVERSITY OF CALIFORNIA COOPERATIVE EXTENSION   (United States)

^c UNIVERSITY OF CALIFORNIA   (United States)

^d PIONEER HI BRED INTERNATIONAL INC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

FUNGAL DNA;

ARTICLE; EQUIPMENT; GENETICS; ISOLATION AND PURIFICATION; METHODOLOGY; MICROBIOLOGY; PLANT DISEASE; PLANT SEED; REAL TIME POLYMERASE CHAIN REACTION; REPRODUCIBILITY; SENSITIVITY AND SPECIFICITY; SPINACH; VERTICILLIUM;

DNA, FUNGAL; PLANT DISEASES; REAL-TIME POLYMERASE CHAIN REACTION; REPRODUCIBILITY OF RESULTS; SEEDS; SENSITIVITY AND SPECIFICITY; SPINACIA OLERACEA; VERTICILLIUM;

FUNGI; LACTUCA; SPINACIA OLERACEA; VERTICILLIUM DAHLIAE;

EID: 84858977896     PISSN: 0031949X     EISSN: None     Source Type: Journal    
DOI: 10.1094/PHYTO-10-11-0280     Document Type: Article

References (46)

1. Atallah, Z. K., Bae, J., Jansky, S. H., Rouse, D. I., and Stevenson, W. R. 2007. Multiplex real-time quantitative PCR to detect and quantify Verticillium dahliae colonization in potato lines that differ in response to Verticillium wilt. Phytopathology 97:865-872. (Pubitemid 47035278)
2. Atallah, Z. K., Maruthachalam, K., du Toit, L., Koike, S. T., Michael Davis, R., Klosterman, S. J., Hayes, R. J., and Subbarao, K. V. 2010. Population analyses of the vascular plant pathogen Verticillium dahliae detect recombination and transcontinental gene flow. Fungal Genet. Biol. 47:416-422.
3. Bates, J. A., Taylor, E. J. A., Kenyon, D. M., and Thomas, J. E. 2001.The application of real-time PCR to the identification, detection and quantification of Pyrenophora species in barley seed. Mol. Plant Pathol. 2:49-57. (Pubitemid 32442584)
4. Bhat, R. G., and Subbarao, K.V. 1999. Host range specificity in Verticillium dahliae. Phytopathology 89:1218-1225.
5. Brandfass, C., and Karlovsky, P. 2008. Upscaled CTAB-based DNA extraction and real-time PCR assays for Fusarium culmorum and Fusarium graminearum DNA in plant material with reduced sampling error. Int. J. Mol. Sci. 9:2306-2321.
6. Broders, K. D., and Boland, G. J. 2010. Molecular diagnostic assay for detection of the butternut canker pathogen Sirococcus clavigignentijuglandacearum. Plant Dis. 94:952-958.
7. Bustin, S. A., Benes, V., Garson, J. A., Hellemans, J., Huggett, J., Kubista, M., Mueller, R., Nolan, T., Pfaffl, M. W., Shipley, G. L., Vandesompele, J., and Wittwer, C. T. 2009. The MIQE guidelines: Minimum information for publication of quantitative real-time PCR experiments. Clin. Chem. 55:611-622.
8. Chilvers, M. I., du Toit, L. J., Akamatsu, H., and Peever, T. L. 2007. A real-time, quantitative PCR seed assay for Botrytis spp. that cause neck rot of onion. Plant Dis. 91:599-608. (Pubitemid 46649676)
9. Cho, M. S., Kang, M. J., Kim, C. K., Seol, Y.-J., Hahn, J. H., Park, S. C., Hwang, D. J., Ahn, T.-Y., Park, D. H., Lim, C. K., and Park, D. S. 2011. Sensitive and specific detection of Xanthomonas oryzae pv. oryzae by real-time bio-PCR using pathovar-specific primers based on an rhs family gene. Plant Dis. 95:589-594.
10. Diguta, C. F., Rousseaux, S., Weidmann, S., Bretin, N., Vincent, B., Guilloux-Benatier, M., and Alexandre, H. 2010. Development of a qPCR assay for specific quantification of Botrytis cinerea on grapes. FEMS MicroBiol. Lett. 313:81-87.
11. du Toit, L. J., Derie, M. L., and Hernandez-Perez, P. 2005. Verticillium wilt in spinach seed production. Plant Dis. 89:4-11. (Pubitemid 40047828)
12. Eynck, C., Koopmann, B., Grunewaldt-Stoecker, G., Karlovsky, P., and von Tiedemann, A. 2007. Differential interactions of Verticillium longisporum and Verticillium dahliae with Brassica napus detected with molecular and histological techniques. Eur. J. Plant Pathol. 118:259-274.
13. Glynn, N. C., and Edwards, S. G. 2010. Evaluation of PCR assays for quantifying seed-borne infection by Fusarium and Microdochium seedling blight pathogens. J. Appl. MicroBiol. 108:81-87.
14. Glynn, N. C., Ray, R., Edwards, S. G., Hare, M. C., Parry, D. W., Barnett, C. J., and Beck, J. J. 2007. Quantitative Fusarium spp. and Microdochium spp. PCR assays to evaluate seed treatments for the control of Fusarium seedling blight of wheat. J. Appl. MicroBiol. 102:1645-1653. (Pubitemid 46934588)
15. Gudmestad, N. C., Mallik, I., Pasche, J. S., Anderson, N. R., and Kinzer, K. 2009. A real-time PCR assay for the detection of Clavibacter michiganensis subsp. sepedonicus based on the cellulase A gene sequence. Plant Dis. 93:649-659.
16. Guillemette, T., Iacomi-Vasilescu, B. A., and Simoneau, P. 2004. Conventional and real-time PCR-based assay for detecting pathogenic Alternaria brassicae in cruciferous seed. Plant Dis. 88:490-496. (Pubitemid 38504380)
17. Ha, Y., Fessehaie, A., Ling, K. S., Wechter, W. P., Keinath, A. P., and Walcott, R. R. 2009. Simultaneous detection of Acidovorax avenae subsp. citrulli and Didymella bryoniae in cucurbit seed lots using magnetic capture hybridization and real-time polymerase chain reaction. Phytopathology 99:666-678.
18. Heale, J. B., and Karapapa, V. K. 1999. The Verticillium threat to Canada's major oilseed crop: Canola. Can. J. Plant Pathol. 21:1-7. (Pubitemid 29294232)
19. Inderbitzin, P., Davis, R. M., Bostock, R. M., and Subbarao, K. V. 2011. The ascomycete Verticillium longisporum is a hybrid and a plant pathogen with an expanded host range. PLoS ONE 6:18260.
20. Ioos, R., Fourrier, C. l., Iancu, G., and Gordon, T. R. 2009. Sensitive detection of Fusarium circinatum in pine seed by combining an enrichment procedure with a real-time polymerase chain reaction using duallabeled probe chemistry. Phytopathology 99:582-590.
21. IPC. 2003. International phytosanitary certificate no. 4051. Phytosanitary federal law of the Mexican United States.
22. Justesen, A., Hansen, H., and Pinnschmidt, H. 2008. Quantification of Pyrenophora graminea in barley seed using real-time PCR. Eur. J. Plant Pathol. 122:253-263.
23. Karajeh, M. R., and Masoud, S. A. 2006. Molecular detection of Verticillium dahliae Kleb. in asymptomatic olive trees. J. PhytoPathol. 154:496-499. (Pubitemid 44114737)
24. Karapapa, V. K., Bainbridge, B. W., and Heale, J. B. 1997. Morphological and molecular characterization of Verticillium longisporum comb. nov. pathogenic to oilseed rape. Mycol. Res. 101:1281-1294. (Pubitemid 28197128)
25. Klosterman, S. J., Atallah, Z. K., Vallad, G. E., and Subbarao, K. V. 2009. Diversity, pathogenicity, and management of Verticillium species. Annu. Rev. PhytoPathol. 47:39-62.
26. Li, K. N., Rouse, D. I., Eyestone, E. J., and German, T. L. 1999. The generation of specific DNA primers using random amplified polymorphic DNA and its application to Verticillium dahliae. Mycol. Res. 103:1361-1368. (Pubitemid 30319426)
27. Lievens, B., Brouwer, M., Vanachter, A. C. R. C., Cammue, B. P. A., and Thomma, B. P. H. J. 2006. Real-time PCR for detection and quantification of Fungal and oomycete tomato pathogens in plant and soil samples. Plant Sci. 171:155-165. (Pubitemid 43744528)
28. Ma, Z., and Michailides, T. J. 2007. Approaches for eliminating PCR inhibitors and designing PCR primers for the detection of phytopathogenic fungi. Crop Prot. 26:145-161. (Pubitemid 44881150)
29. Mahuku, G., and Platt, H. 2002. Quantifying Verticillium dahliae in soils collected from potato fields using a competitive PCR assay. Am. J. Potato Res. 79:107-117. (Pubitemid 34294122)
30. Malvick, D. K., and Grunden, E. 2005. Isolation of Fungal DNA from plant tissues and removal of DNA amplification inhibitors. Mol. Ecol. Notes 5:958-960. (Pubitemid 43278021)
31. Malvick, D. K., and Impullitti, A. E. 2007. Detection and quantification of Phialophora gregata in soybean and soil samples with a quantitative realtime PCR assay. Plant Dis. 91:736-742. (Pubitemid 46789737)
32. Mbofung, G. C. Y., and Pryor, B. M. 2010. A PCR-based assay for detection of Fusarium oxysporum f. sp. lactucae in lettuce seed. Plant Dis. 94:860-866.
33. McNeil, M., Roberts, A. M. I., Cockerell, V., and Mulholland, V. 2004. Real-time PCR assay for quantification of Tilletia caries contamination of UK wheat seed. Plant Pathol. 53:741-750. (Pubitemid 40047556)
34. Montes-Borrego, M., Muã-Ledesma, F. J., Jim, R. M., and Landa, B. B. Real-time PCR quantification of Peronospora arborescens, the opium poppy downy mildew pathogen, in seed stocks and symptomless infected plants. Plant Dis. 95:143-152.
35. Okubara, P. A., Schroeder, K. L., and Paulitz, T. C. 2008. Identification and quantification of Rhizoctonia solani and Rhizoctonia oryzae using real-time polymerase chain reaction. Phytopathology 98:837-847. (Pubitemid 352018218)
36. Pegg, B., and Brady, G. 2002. Verticillium Wilts. CABI Publishing, New York.
37. Pryor, B. M., and Gilbertson, R. L. 2001. A PCR-based assay for detection of Alternaria radicina on carrot seed. Plant Dis. 85:18-23. (Pubitemid 32122283)
38. Qin, Q. M., Vallad, G. E., Wu, B. M., and Subbarao, K. V. 2006. Phylogenetic analyses of phytopathogenic isolates of Verticillium spp. Phytopathology 96:582-592. (Pubitemid 43825522)
39. Radford, A. 1991. Methods in Yeast Genetics: A Laboratory Course Manual. M. Rose, F. Winston, and P. Hieter, eds. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
40. Rampersad, S. N. 2010. Verticillium dahliae (Kleb.) infecting pumpkin seed. J. PhytoPathol. 158:329-333.
41. Sorensen, L. H., Schneider, A. T., and Davis, J. R. 1991. Influence of sodium polygalacturonate sources and improved recovery of Verticillium species from soil. Phytopathology 81:1347.
42. Steventon, L. A., Fahleson, J., Hu, Q., and Dixelius, C. 2002. Identification of the causal agent of Verticillium wilt of winter oilseed rape in Sweden, V. longisporum. Mycol. Res. 106:570-578.
43. Subbarao, K. V., Hubbard, J. C. Greathead, A. S., and Spencer, G. A. 1997. Verticillium wilt. Pages 26-27 in: Compendium of Lettuce Diseases. R. M. Davis, K. V. Subbarao, R. N. Raid, and E. A. Kurtz, eds. The American Phytopathological Society, St. Paul, MN.
44. Tooley, P. W., Carras, M. M., Sechler, A., and Rajasab, A. H. 2010. Realtime PCR detection of sorghum ergot pathogens Claviceps africana, Claviceps sorghi and Claviceps sorghicola. J. PhytoPathol. 158:698-704.
45. Vallad, G. E., Bhat, R. G., Koike, S. T., Ryder, E. J., and Subbarao, K. V. 2005. Weedborne reservoirs and seed transmission of Verticillium dahliae in lettuce. Plant Dis. 89:317-324. (Pubitemid 40335818)
46. Van Gent-Pelzer, M. P. E., Van Brouwershaven, I. R., Kox, L. F. F., and Bonants, P. J. M. 2007. A TaqMan PCR method for routine diagnosis of the quarantine fungus Guignardia citricarpa on citrus fruit. J. PhytoPathol. 155:357-363. (Pubitemid 46763485)