4D imaging of protein aggregation in live cells

Journal of Visualized Experiments

Volumn , Issue 74, 2013, Pages

  Spokoini, Rachel ^a   Shamir, Maya ^a   Keness, Alma ^a   Kaganovich, Daniel ^a  

^a HEBREW UNIVERSITY OF JERUSALEM   (Israel)

Author keywords

4D live cell imaging; Aggregation quality control; Assay; Cell; Cell biology; Cellular biology; Genetics; GFP; Imaging; IPOD (insoluble protein deposit); Issue 74; JUNQ (juxtanuclear quality control compartment); Laser; Live cells; Molecular biology; Protein folding; Protein folding quality control; Proteins; Proteostasis sensor; Yeast

Indexed keywords

EID: 84876009154     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/50083     Document Type: Article

References (12)

1. Gershenson, A. & Gierasch, L.M. Protein folding in the cell: challenges and progress. Current opinion in structural biology. 21, 32-41 (2011).
2. Aguzzi, A. & Calella, A.M. Prions: protein aggregation and infectious diseases. Physiological reviews. 89, 1105-1152 (2009).
3. Morimoto, R.I. Proteotoxic stress and inducible chaperone networks in neurodegenerative disease and aging. Genes & development. 22, 1427 (2008).
4. Cohen, E. & Dillin, A. The insulin paradox: aging, proteotoxicity and neurodegeneration. Nature reviews. Neuroscience. (2008).
5. Hershko, A. & Ciechanover, A. The ubiquitin system. Annual review of biochemistry. 67, 425 (1998).
6. Tyedmers, J., Mogk, A., & Bukau, B. Cellular strategies for controlling protein aggregation. Nature reviews. Molecular cell biology. 11, 777-788 (2010).
7. Treusch, S., Cyr, D.M., & Lindquist, S. Amyloid deposits: Protection against toxic protein species? Cell cycle (Georgetown, Tex.). 8, 1668-1674 (2009).
8. Spokoini, R., et al. Confinement to Organelle-Associated Inclusion Structures Mediates Asymmetric Inheritance of Aggregated Protein in Budding Yeast. Cell Rep. doi:S2211-1247(12)00264-1 [pii] 10.1016/j.celrep.2012.08.024 (2012).
9. Weisberg, S.J., et al. Compartmentalization of superoxide dismutase 1 (SOD1G93A) aggregates determines their toxicity. Proc Natl Acad Sci U S A. 109, 15811-15816 (2012).
10. Kaganovich, D., Kopito, R., & Frydman, J. Misfolded proteins partition between two distinct quality control compartments. Nature. 454, 1088 (2008).
11. Betting, J. & Seufert, W. A yeast Ubc9 mutant protein with temperature-sensitive in vivo function is subject to conditional proteolysis by a ubiquitin-and proteasome-dependent pathway. The Journal of biological chemistry. 271, 25790 (1996).
12. Tongaonkar, P., Beck, K., Shinde, U.P., & Madura, K. Characterization of a temperature-sensitive mutant of a ubiquitin-conjugating enzyme and its use as a heat-inducible degradation signal. Analytical biochemistry. 272, 263 (1999).