Characterization of a temperature-sensitive mutant of a ubiquitin- conjugating enzyme and its use as a heat-inducible degradation signal

Analytical Biochemistry

Volumn 272, Issue 2, 1999, Pages 263-269

  Tongaonkar, Prasad ^a   Beck, Konrad ^a,b   Shinde, Ujwal P ^a   Madura, Kiran ^a  

^a RUTGERS ROBERT WOOD JOHNSON MEDICAL SCHOOL   (United States)

^b NAGOYA UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

CATALYST ACTIVITY; DEFECTS; SUBSTRATES;

BIOPHYSICAL PROPERTIES; GLUTATHIONE S-TRANSFERASES; SITE DIRECTED MUTAGENESIS; SUBSTRATE RECOGNITION; TEMPERATURE DEPENDENT; TEMPERATURE SENSITIVE; TEMPERATURE-SENSITIVE GROWTH; UBIQUITIN-CONJUGATING ENZYMES;

ENZYMES;

GLUTATHIONE TRANSFERASE; PROTEASOME; UBIQUITIN CONJUGATING ENZYME;

ARTICLE; CATALYSIS; CIRCULAR DICHROISM; ENZYME ANALYSIS; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN STABILITY; SIGNAL TRANSDUCTION; SITE DIRECTED MUTAGENESIS; TEMPERATURE DEPENDENCE; TEMPERATURE SENSITIVE MUTANT;

EUKARYOTA;

EID: 0033180153     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.1999.4190     Document Type: Article

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