Characterization of an efficientdengue virus replicon for development of assays of discovery of small molecules against dengue virus

Antiviral Research

Volumn 98, Issue 2, 2013, Pages 228-241

  Yang, Chi Chen ^a   Tsai, Ming Han ^a   Hu, Han Shu ^a   Pu, Szu Yuan ^a,b   Wu, Ren Huang ^a,b   Wu, Szu Huei ^a   Lin, Hui Mei ^a   Song, Jen Shin ^a   Chao, Yu Sheng ^a   Yueh, Andrew ^a,b  

^a NATIONAL HEALTH RESEARCH INSTITUTES   (Taiwan)

^b Graduate Institute of Life Sciences at TKU   (Taiwan)

Author keywords

Dengue replicon; Dengue virus; DNA launched dengue replicon; High throughput screening

Indexed keywords

DENGUE VACCINE; MYCOPHENOLIC ACID; RENILLA LUCIFERIN 2 MONOOXYGENASE; VIRUS RNA; ANTIVIRUS AGENT;

ANIMAL CELL; ARTICLE; DENGUE VIRUS; DENGUE VIRUS 2; HIGH THROUGHPUT SCREENING; NONHUMAN; PRIORITY JOURNAL; REPLICON; REPORTER GENE; RNA REPLICATION; STABLE EXPRESSION; VIRUS CAPSID; VIRUS REPLICATION; ANIMAL; BIOASSAY; DENGUE; DRUG EFFECT; DRUG SCREENING; EVALUATION; GENETICS; HUMAN; METABOLISM; METHODOLOGY; MOLECULAR LIBRARY; PHYSIOLOGY; VIROLOGY;

DENGUE VIRUS; MIRIDAE; RENILLA LUCIFERASE;

ANIMALS; ANTIVIRAL AGENTS; BIOLOGICAL ASSAY; DENGUE; DENGUE VIRUS; DRUG EVALUATION, PRECLINICAL; GENES, REPORTER; HUMANS; LUCIFERASES, RENILLA; REPLICON; SMALL MOLECULE LIBRARIES; VIRUS REPLICATION;

EID: 84875999612     PISSN: 01663542     EISSN: 18729096     Source Type: Journal    
DOI: 10.1016/j.antiviral.2013.03.001     Document Type: Article

References (140)

1. Alvarez, D.E.,DeLella Ezcurra,A.L.,Fucito,S.,Gamarnik,A.V.,2005a.Role of RNA structures present at the 30UTR of dengue virus ontranslation,RNA synthesis, and viral replication.Virology 339,200-212.
2. Alvarez D.E., De Lella Ezcurra A.L., Fucito S., Gamarnik A.V. Role of RNA structures present at the 3'UTR of dengue virus on translation, RNA synthesis, and viral replication. Virology 2005, 339:200-212.
3. Alvarez, D.E.,Filomatori,C.V.,Gamarnik,A.V.,2008. Functional analysis of dengue virus cyclization sequences located at the 50 and 30 UTRs. Virology 375,223-235.
4. Alvarez D.E., Filomatori C.V., Gamarnik A.V. Functional analysis of dengue virus cyclization sequences located at the 5' and 3'UTRs. Virology 2008, 375:223-235.
5. Alvarez, D.E.,Lodeiro,M.F.,Luduena,S.J.,Pietrasanta,L.I.,Gamarnik,A.V.,2005b. Long-range RNA-RNA interactions circularize the dengue virus genome.Journal of Virology 79,6631-6643.
6. Alvarez D.E., Lodeiro M.F., Luduena S.J., Pietrasanta L.I., Gamarnik A.V. Long-range RNA-RNA interactions circularize the dengue virus genome. Journal of Virology 2005, 79:6631-6643.
7. Ansarah-Sobrinho, C.,Nelson,S.,Jost,C.A.,Whitehead,S.S.,Pierson,T.C.,2008. Temperature-dependent production of pseudoinfectious dengue reporter virus particles by complementation.Virology 381,67-74.
8. Ansarah-Sobrinho C., Nelson S., Jost C.A., Whitehead S.S., Pierson T.C. Temperature-dependent production of pseudoinfectious dengue reporter virus particles by complementation. Virology 2008, 381:67-74.
9. Blight, K.J.,Kolykhalov,A.A.,Rice,C.M.,2000.EfficientInitiation of HCV RNA Replication in Cell Culture.Science,New York,NY,290,1972-1974.
10. Blight K.J., Kolykhalov A.A., Rice C.M. Efficient Initiation of HCV RNA Replication in Cell Culture 2000, Science, New York, NY, 290, 1972-1974.
11. Burke, D.,2000.Methods inYeast Genetics:aCold Spring Harbor Laboratory Course Manual, 2000 ed.Cold Spring Harbor Laboratory Press,Plainview,NY.
12. Burke D. Methods in Yeast Genetics: a Cold Spring Harbor Laboratory Course Manual 2000, Cold Spring Harbor Laboratory Press, Plainview, NY. 2000 ed.
13. Calisher, C.H.,Karabatsos,N.,Dalrymple,J.M.,Shope,R.E.,Porterfield,J.S., Westaway, E.G.,Brandt,W.E.,1989.Antigenic relationships between flaviviruses asdetermined bycross-neutralization tests with polyclonal antisera. The Journal of General Virology 70(Pt1),37-43.
14. Calisher C.H., Karabatsos N., Dalrymple J.M., Shope R.E., Porterfield J.S., Westaway E.G., Brandt W.E. Antigenic relationships between flaviviruses as determined by cross-neutralization tests with polyclonal antisera. The Journal of General Virology 1989, 70(Pt 1):37-43.
15. Cao, F.,Li,X.F.,Yu,X.D.,Deng,Y.Q.,Jiang,T.,Zhu,Q.Y.,Qin,E.D.,Qin,C.F.,2011.A DNA-based West Nile virus replicon elicits humoral and cellular immune responses in mice.Journal of Virological Methods 178,87-93.
16. Cao F., Li X.F., Yu X.D., Deng Y.Q., Jiang T., Zhu Q.Y., Qin E.D., Qin C.F. A DNA-based West Nile virus replicon elicits humoral and cellular immune responses in mice. Journal of Virological Methods 2011, 178:87-93.
17. Carroll, S.S.,Tomassini,J.E.,Bosserman,M.,Getty,K.,Stahlhut,M.W.,Eldrup,A.B., Bhat, B.,Hall,D.,Simcoe,A.L.,LaFemina,R.,Rutkowski,C.A.,Wolanski,B.,Yang, Z., Migliaccio,G.,DeFrancesco,R.,Kuo,L.C.,MacCoss,M.,Olsen,D.B.,2003. Inhibition of hepatitis Cvirus RNA replication by 20-modified nucleoside analogs. The Journal of Biological Chemistry 278,11979-11984.
18. Carroll S.S., Tomassini J.E., Bosserman M., Getty K., Stahlhut M.W., Eldrup A.B., Bhat B., Hall D., Simcoe A.L., LaFemina R., Rutkowski C.A., Wolanski B., Yang Z., Migliaccio G., De Francesco R., Kuo L.C., MacCoss M., Olsen D.B. Inhibition of hepatitis C virus RNA replication by 2'-modified nucleoside analogs. The Journal of Biological Chemistry 2003, 278:11979-11984.
19. Chambers, T.J.,Hahn,C.S.,Galler,R.,Rice,C.M.,1990.Flavivirus genome organization, expression, and replication.Annual Review of Microbiology 44, 649-688.
20. Chambers T.J., Hahn C.S., Galler R., Rice C.M. Flavivirus genome organization, expression, and replication. Annual Review of Microbiology 1990, 44:649-688.
21. Chiu, W.W.,Kinney,R.M.,Dreher,T.W.,2005.Control of translation bythe 50- and 30-terminal regions of the dengue virus genome.Journal of Virology 79,8303-8315.
22. Chiu W.W., Kinney R.M., Dreher T.W. Control of translation by the 5'- and 3'-terminal regions of the dengue virus genome. Journal of Virology 2005, 79:8303-8315.
23. Clyde, K.,Barrera,J., Harris E.,2008.The capsid-coding region hairpin element (cHP) isacritical determinant of dengue virus and West Nile virus RNA synthesis. Virology 379,314-323.
24. Clyde K., Barrera J., Harris E. The capsid-coding region hairpin element (cHP) is a critical determinant of dengue virus and West Nile virus RNA synthesis. Virology 2008, 379:314-323.
25. Clyde, K., Harris E.,2006.RNA secondary structure in the coding region of dengue virus type 2 directs translation start codon selection and is required for viral replication. Journal of Virology 80,2170-2182.
26. Clyde K., Harris E. RNA secondary structure in the coding region of dengue virus type 2 directs translation start codon selection and is required for viral replication. Journal of Virology 2006, 80:2170-2182.
27. Diamond, M.S.,Zachariah,M., Harris E.,2002.Mycophenolic acid inhibits dengue virus infection by preventing replication of viral RNA.Virology 304,211-221.
28. Diamond M.S., Zachariah M., Harris E. Mycophenolic acid inhibits dengue virus infection by preventing replication of viral RNA. Virology 2002, 304:211-221.
29. Falgout, B.,Pethel,M.,Zhang,Y.M.,Lai,C.J.,1991.Both nonstructural proteins NS2B and NS3 are required for the proteolytic processing of dengue virus nonstructural proteins. Journal of Virology 65,2467-2475.
30. Falgout B., Pethel M., Zhang Y.M., Lai C.J. Both nonstructural proteins NS2B and NS3 are required for the proteolytic processing of dengue virus nonstructural proteins. Journal of Virology 1991, 65:2467-2475.
31. Fayzulin, R.,Scholle,F.,Petrakova,O.,Frolov,I.,Mason,P.W.,2006.Evaluation of replicative capacity and genetic stability of West Nile virus replicons using highly efficient packaging cell lines.Virology 351,196-209.
32. Fayzulin R., Scholle F., Petrakova O., Frolov I., Mason P.W. Evaluation of replicative capacity and genetic stability of West Nile virus replicons using highly efficient packaging cell lines. Virology 2006, 351:196-209.
33. Friebe, P., Harris E.,2010.Interplay of RNA elements inthe dengue virus 50 and 30 ends required for viral RNA replication.Journal of Virology 84,6103-6118.
34. Friebe P., Harris E. Interplay of RNA elements in the dengue virus 5' and 3' ends required for viral RNA replication. Journal of Virology 2010, 84:6103-6118.
35. Friebe, P.,Pena,J.,Pohl,M.O., Harris E.,2012.Composition of the sequence downstream of the dengue virus 50 cyclization sequence (dCS)affects viral RNA replication. Virology 422,346-356.
36. Friebe P., Pena J., Pohl M.O., Harris E. Composition of the sequence downstream of the dengue virus 5' cyclization sequence (dCS) affects viral RNA replication. Virology 2012, 422:346-356.
37. Friebe, P.,Shi,P.Y., Harris E.,2011.The 50 and 30 downstream AUG region elements are required for mosquito-borne flavivirusRNA replication.Journal of Virology 85, 1900-1905.
38. Friebe P., Shi P.Y., Harris E. The 5' and 3' downstream AUG region elements are required for mosquito-borne flavivirus RNA replication. Journal of Virology 2011, 85:1900-1905.
39. Gietz, R.D.,Woods,R.A.,2002.Transformation of yeast bylithium acetate/single-stranded carrier DNA/polyethylene glycol method.Methods inEnzymology 350, 87-96.
40. Gietz R.D., Woods R.A. Transformation of yeast by lithium acetate/single-stranded carrier DNA/polyethylene glycol method. Methods in Enzymology 2002, 350:87-96.
41. Groat-Carmona, A.M.,Orozco,S.,Friebe,P.,Payne,A.,Kramer,L., Harris E.,2012.A novel coding-region RNA element modulates infectious dengue virus particle production inboth mammalian and mosquito cells and regulates viral replication in Aedes aegypti mosquitoes.Virology 432,511-526.
42. Groat-Carmona A.M., Orozco S., Friebe P., Payne A., Kramer L., Harris E. A novel coding-region RNA element modulates infectious dengue virus particle production in both mammalian and mosquito cells and regulates viral replication in Aedes aegypti mosquitoes. Virology 2012, 432:511-526.
43. Gubler, D.J.,1989.Aedes aegypti and Aedes aegypti-borne disease control inthe 1990s: top down orbottom up.Charles Franklin Craig Lecture.The American Journal of Tropical Medicine and Hygiene 40,571-578.
44. Gubler D.J. Aedes aegypti and Aedes aegypti-borne disease control in the 1990s: top down or bottom up. Charles Franklin Craig Lecture. The American Journal of Tropical Medicine and Hygiene 1989, 40:571-578.
45. Guzman, M.G.,Kouri,G.,2002.Dengue:anupdate.The Lancet Infectious Diseases 2, 33-42.
46. Guzman M.G., Kouri G. Dengue: an update. The Lancet Infectious Diseases 2002, 2:33-42.
47. Guzman, M.G.,Kouri,G.,2004.Dengue diagnosis,advances and challenges. International Journal of Infectious Diseases 8,69-80.
48. Guzman M.G., Kouri G. Dengue diagnosis, advances and challenges. International Journal of Infectious Diseases 2004, 8:69-80.
49. Hahn, C.S.,Hahn,Y.S.,Rice,C.M.,Lee,E.,Dalgarno,L.,Strauss,E.G.,Strauss,J.H.,1987. Conserved elements inthe 30 untranslated region of flavivirusRNAs and potential cyclization sequences. Journal of Molecular Biology 198,33-41.
50. Hahn C.S., Hahn Y.S., Rice C.M., Lee E., Dalgarno L., Strauss E.G., Strauss J.H. Conserved elements in the 3' untranslated region of flavivirus RNAs and potential cyclization sequences. Journal of Molecular Biology 1987, 198:33-41.
51. Halstead, S.B.,1980.Dengue haemorrhagic fever-apublic health problem and a field for research.Bulletin of the World Health Organization 58,1-21.
52. Halstead S.B. Dengue haemorrhagic fever-a public health problem and a field for research. Bulletin of the World Health Organization 1980, 58:1-21.
53. Holden, K.L., Harris E.,2004.Enhancement of dengue virus translation:role of the 30 untranslated region and the terminal 30 stem-loop domain. Virology 329,119-133.
54. Holden K.L., Harris E. Enhancement of dengue virus translation: role of the 3' untranslated region and the terminal 3' stem-loop domain. Virology 2004, 329:119-133.
55. Holden, K.L.,Stein,D.A.,Pierson,T.C.,Ahmed,A.A.,Clyde,K.,Iversen,P.L., Harris E.,2006. Inhibition of dengue virus translation and RNA synthesis byamorpholino oligomer targeted to the top of the terminal 30 stem-loop structure. Virology 344,439-452.
56. Holden K.L., Stein D.A., Pierson T.C., Ahmed A.A., Clyde K., Iversen P.L., Harris E. Inhibition of dengue virus translation and RNA synthesis by a morpholino oligomer targeted to the top of the terminal 3' stem-loop structure. Virology 2006, 344:439-452.
57. Houng, H.H.,Hritz,D.,Kanesa-thasan,N.,2000.Quantitative detection of dengue 2 virus using fluorogenicRT-PCR based on30-noncoding sequence. Journal of Virological Methods 86,1-11.
58. Houng H.H., Hritz D., Kanesa-thasan N. Quantitative detection of dengue 2 virus using fluorogenic RT-PCR based on 3'-noncoding sequence. Journal of Virological Methods 2000, 86:1-11.
59. Hsu, Y.C.,Chen,N.C.,Chen,P.C.,Wang,C.C.,Cheng,W.C.,Wu,H.N.,2012. Identification of asmall-molecule in hibitor of dengue virus using areplicon system. Archives of Virology 157,681-688.
60. Hsu Y.C., Chen N.C., Chen P.C., Wang C.C., Cheng W.C., Wu H.N. Identification of a small-molecule inhibitor of dengue virus using a replicon system. Archives of Virology 2012, 157:681-688.
61. Huang, Y.,Liu,S.,Yang,P.,Wang,C.,Du,Y.,Yu,W.,Sun,Z.,2012.Replicon-based Japanese encephalitis virus vaccines elicit immune response inmice.Journal of Virological Methods 179, 217-225.
62. Huang Y., Liu S., Yang P., Wang C., Du Y., Yu W., Sun Z. Replicon-based Japanese encephalitis virus vaccines elicit immune response in mice. Journal of Virological Methods 2012, 179:217-225.
63. Jones, M.,Davidson,A.,Hibbert,L.,Gruenwald,P.,Schlaak,J.,Ball,S.,Foster,G.R., Jacobs, M.,2005.Dengue virus inhibits alpha interferon signaling byreducing STAT2 expression. Journal of Virology 79,5414-5420.
64. Jones M., Davidson A., Hibbert L., Gruenwald P., Schlaak J., Ball S., Foster G.R., Jacobs M. Dengue virus inhibits alpha interferon signaling by reducing STAT2 expression. Journal of Virology 2005, 79:5414-5420.
65. Khromykh, A.A.,Meka,H.,Guyatt,K.J.,Westaway,E.G.,2001.Essential role of cyclization sequences in flavivirus RNA replication.Journal of Virology 75, 6719-6728.
66. Khromykh A.A., Meka H., Guyatt K.J., Westaway E.G. Essential role of cyclization sequences in flavivirus RNA replication. Journal of Virology 2001, 75:6719-6728.
67. Leardkamolkarn, V.,Sirigulpanit,W.,2012.Establishment of astable cell line coexpressing dengue virus-2 and green fluorescentprotein for screening of antiviral compounds. Journal of Bimolecular Screening 17,283-292.
68. Leardkamolkarn V., Sirigulpanit W. Establishment of a stable cell line coexpressing dengue virus-2 and green fluorescent protein for screening of antiviral compounds. Journal of Bimolecular Screening 2012, 17:283-292.
69. Leardkamolkarn, V.,Sirigulpanit,W.,Chotiwan,N.,Kumkate,S.,Huang,C.Y.,2012. Development of Dengue type-2 virus replicons expressing GFP reporter gene in study of viral RNA replication.Virus Research 163,552-562.
70. Leardkamolkarn V., Sirigulpanit W., Chotiwan N., Kumkate S., Huang C.Y. Development of Dengue type-2 virus replicons expressing GFP reporter gene in study of viral RNA replication. Virus Research 2012, 163:552-562.
71. Lee, T.C.,Lin,Y.L.,Liao,J.T.,Su,C.M.,Lin,C.C.,Lin,W.P.,Liao,C.L.,2010.Utilizing liver-specific microRNA-122 tomodulate replication of dengue virus replicon. Biochemical and Biophysical Research Communications 396,596-601.
72. Lee T.C., Lin Y.L., Liao J.T., Su C.M., Lin C.C., Lin W.P., Liao C.L. Utilizing liver-specific microRNA-122 to modulate replication of dengue virus replicon. Biochemical and Biophysical Research Communications 2010, 396:596-601.
73. Lescar, J.,Luo,D.,Xu,T.,Sampath,A.,Lim,S.P.,Canard,B.,Vasudevan,S.G.,2008. Towards the design of antiviral in hibitors against flaviviruses:the case for the multifunctional NS3 protein from dengue virus asatarget.Antiviral Research 80, 94-101.
74. Lescar J., Luo D., Xu T., Sampath A., Lim S.P., Canard B., Vasudevan S.G. Towards the design of antiviral inhibitors against flaviviruses: the case for the multifunctional NS3 protein from dengue virus as a target. Antiviral Research 2008, 80:94-101.
75. Lindenbach, B.D.,Thiel,H.J.,Rice,C.M.,2007.Flaviviridae:The Virus and their Replication, Fields Virology,5th ed.Lippincott Williams &Wilkins, Philadelphia, PA,1101-1152.
76. Lindenbach B.D., Thiel H.J., Rice C.M. Flaviviridae: The Virus and their Replication, Fields Virology 2007, Lippincott Williams & Wilkins, Philadelphia, PA, 1101-1152. 5th ed.
77. Lohmann, V.,Korner,F.,Koch,J.,Herian,U.,Theilmann,L.,Bartenschlager,R.,1999. Replication of subgenomic hepatitis Cvirus RNAs inahepatoma cell line. Science, New York,NY,285,110-113.
78. Lohmann V., Korner F., Koch J., Herian U., Theilmann L., Bartenschlager R. Replication of subgenomic hepatitis C virus RNAs in a hepatoma cell line 1999, Science, New York, NY, 285, 110-113.
79. Masse, N.,Davidson,A.,Ferron,F.,Alvarez,K.,Jacobs,M.,Romette,J.L.,Canard,B., Guillemot, J.C.,2010.Dengue virus replicons:production of aninterserotypic chimera and cell lines from different species, and establishment of acell-based fluorescent assay toscreen in hibitors,validated bythe evaluation of ribavirin's activity. Antiviral Research 86,296-305.
80. Masse N., Davidson A., Ferron F., Alvarez K., Jacobs M., Romette J.L., Canard B., Guillemot J.C. Dengue virus replicons: production of an interserotypic chimera and cell lines from different species, and establishment of a cell-based fluorescent assay to screen inhibitors, validated by the evaluation of ribavirin's activity. Antiviral Research 2010, 86:296-305.
81. Men, R.,Bray,M.,Clark,D.,Chanock,R.M.,Lai,C.J.,1996.Dengue type 4virus mutants containing deletions inthe 3'noncoding region of the RNA genome: analysis of growth restriction incell culture and altered viremia pattern and immunogenicity in rhesus monkeys.Journal of Virology 70,3930-3937.
82. Men R., Bray M., Clark D., Chanock R.M., Lai C.J. Dengue type 4 virus mutants containing deletions in the 3' noncoding region of the RNA genome: analysis of growth restriction in cell culture and altered viremia pattern and immunogenicity in rhesus monkeys. Journal of Virology 1996, 70:3930-3937.
83. Miroux, B.,Walker,J.E.,1996.Over-production of proteins in Escherichia coli : mutant hosts that allow synthesis of some membrane proteins and globular proteins at high levels.Journal of Molecular Biology 260,289-298.
84. Miroux B., Walker J.E. Over-production of proteins in Escherichia coli: mutant hosts that allow synthesis of some membrane proteins and globular proteins at high levels. Journal of Molecular Biology 1996, 260:289-298.
85. Moradpour, D.,Penin,F.,Rice,C.M.,2007.Replication of hepatitis Cvirus.Nature Reviews Microbiology 5,453-463.
86. Moradpour D., Penin F., Rice C.M. Replication of hepatitis C virus. Nature Reviews Microbiology 2007, 5:453-463.
87. Mosimann, A.L.,deBorba,L.,Bordignon,J.,Mason,P.W.,dos Santos,C.N.,2010. Construction and characterization of astable subgenomic replicon system of a Brazilian dengue virus type 3strain (BRDEN3 290-02).Journal of Virological Methods 163, 147-152.
88. Mosimann A.L., de Borba L., Bordignon J., Mason P.W., dos Santos C.N. Construction and characterization of a stable subgenomic replicon system of a Brazilian dengue virus type 3 strain (BR DEN3 290-02). Journal of Virological Methods 2010, 163:147-152.
89. Ng, C.Y.,Gu,F.,Phong,W.Y.,Chen,Y.L.,Lim,S.P.,Davidson,A.,Vasudevan,S.G.,2007. Construction and characterization of astable subgenomic dengue virus type 2 replicon system for antiviral compound and siRNA testing.Antiviral Research 76, 222-231.
90. Ng C.Y., Gu F., Phong W.Y., Chen Y.L., Lim S.P., Davidson A., Vasudevan S.G. Construction and characterization of a stable subgenomic dengue virus type 2 replicon system for antiviral compound and siRNA testing. Antiviral Research 2007, 76:222-231.
91. Noble, C.G.,Chen,Y.L.,Dong,H.,Gu,F.,Lim,S.P.,Schul,W.,Wang,Q.Y.,Shi,P.Y., 2010. Strategies fordevelopment of dengue virus in hibitors.Antiviral Research 85, 450-462.
92. Noble C.G., Chen Y.L., Dong H., Gu F., Lim S.P., Schul W., Wang Q.Y., Shi P.Y. Strategies f or development of dengue virus inhibitors. Antiviral Research 2010, 85:450-462.
93. Pang, X.,Zhang,M.,Dayton,A.I.,2001.Development of Dengue virus type 2 replicons capable of prolonged expression inhost cells.BMC Microbiology 1,18.
94. Pang X., Zhang M., Dayton A.I. Development of Dengue virus type 2 replicons capable of prolonged expression in host cells. BMC Microbiology 2001, 1:18.
95. Paranjape, S.M., Harris E.,2010.Control of dengue virus translation and replication. Current Topics in Microbiology and Immunology 338,15-34.
96. Paranjape S.M., Harris E. Control of dengue virus translation and replication. Current Topics in Microbiology and Immunology 2010, 338:15-34.
97. Pietschmann, T.,Lohmann,V.,Rutter,G.,Kurpanek,K.,Bartenschlager,R.,2001. Characterization of cell lines carrying self-replicating hepatitis Cvirus RNAs. Journal of Virology 75,1252-1264.
98. Pietschmann T., Lohmann V., Rutter G., Kurpanek K., Bartenschlager R. Characterization of cell lines carrying self-replicating hepatitis C virus RNAs. Journal of Virology 2001, 75:1252-1264.
99. Pinheiro, F.P.,1989.Dengue inthe Americas.1980-1987.Epidemiological Bulletin 10, 1-8.
100. Pinheiro F.P. Dengue in the Americas. 1980-1987. Epidemiological Bulletin 1989, 10:1-8.
101. Pinheiro, F.P.,Corber,S.J.,1997.Global situation of dengue and dengue haemorrhagic fever, and its emergence inthe Americas.World Health Statistics Quarterly 50, 161-169.
102. Pinheiro F.P., Corber S.J. Global situation of dengue and dengue haemorrhagic fever, and its emergence in the Americas. World Health Statistics Quarterly 1997, 50:161-169.
103. Polacek, C.,Foley,J.E., Harris E.,2009.Conformational changes inthe solution structure of the dengue virus 50 end in the presence and absence of the 30 untranslated region. Journal of Virology 83,1161-1166.
104. Polacek C., Foley J.E., Harris E. Conformational changes in the solution structure of the dengue virus 5' end in the presence and absence of the 3' untranslated region. Journal of Virology 2009, 83:1161-1166.
105. Pu, S.Y.,Wu,R.H.,Yang,C.C.,Jao,T.M.,Tsai,M.H.,Wang,J.C.,Lin,H.M.,Chao,Y.S., Yueh, A.,2011.Successful propagation of flavivirusinfectious cDNAs byanovel method to reduce the cryptic bacterial promoter activity of virus genomes. Journal of Virology 85,2927-2941. Qing, M.,Liu,W.,Yuan,Z.,Gu,F.,Shi,P.Y.,2010.Ahigh-throughput assay using dengue-1 virus-like particles for drug discovery.Antiviral Research 86,163- 171.
106. Pu S.Y., Wu R.H., Yang C.C., Jao T.M., Tsai M.H., Wang J.C., Lin H.M., Chao Y.S., Yueh A. Successful propagation of flavivirus infectious cDNAs by a novel method to reduce the cryptic bacterial promoter activity of virus genomes. Journal of Virology 2011, 85:2927-2941.
107. Rice, C.M.,2011.New insights into HCV replication:potential antiviral targets.Top Antiviral Medicine 19,117-120.
108. Qing M., Liu W., Yuan Z., Gu F., Shi P.Y. A high-throughput assay using dengue-1 virus-like particles for drug discovery. Antiviral Research 2010, 86:163-171.
109. Rice, C.M.,Lenches,E.M.,Eddy,S.R.,Shin,S.J.,Sheets,R.L.,Strauss,J.H.,1985.Nucleotide Sequence of Yellow Fever Virus:Implications for Flavivirus Gene Expression and Evolution.Science,New York,NY,229,726-733.
110. Rice C.M. New insights into HCV replication: potential antiviral targets. Top Antiviral Medicine 2011, 19:117-120.
111. Shum, D.,Smith,J.L.,Hirsch,A.J.,Bhinder,B.,Radu,C.,Stein,D.A.,Nelson,J.A.,Fruh, K., Djaballah,H.,2010.High-content assay toidentify in hibitors of dengue virus infection. Assay and Drug Development Technologies 8,553-570.
112. Rice C.M., Lenches E.M., Eddy S.R., Shin S.J., Sheets R.L., Strauss J.H. Nucleotide Sequence of Yellow Fever Virus: Implications for Flavivirus Gene Expression and Evolution 1985, Science, New York, NY, 229, 726-733.
113. Sikorski, R.S.,Hieter,P.,1989.Asystem of shuttle vectors and yeast host strains designed for efficientmanipulation of DNA in Saccharomyces cerevisiae. Genetics 122, 19-27.
114. Shum D., Smith J.L., Hirsch A.J., Bhinder B., Radu C., Stein D.A., Nelson J.A., Fruh K., Djaballah H. High-content assay to identify inhibitors of dengue virus infection. Assay and Drug Development Technologies 2010, 8:553-570.
115. Sriburi, R.,Keelapang,P.,Duangchinda,T.,Pruksakorn,S.,Maneekarn,N.,Malasit,P., Sittisombut, N.,2001.Construction of infectious dengue 2virus cDNA clones using high copy number plasmid.Journal of Virological Methods 92,71-82.
116. Sikorski R.S., Hieter P. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 1989, 122:19-27.
117. Suzuki, R.,deBorba,L.,Duarte dos Santos,C.N.,Mason,P.W.,2007.Construction of an infectious cDNA clone for aBrazilian prototype strain of dengue virus type 1: characterization of atemperature-sensitive mutation in NS1.Virology 362, 374-383.
118. Sriburi R., Keelapang P., Duangchinda T., Pruksakorn S., Maneekarn N., Malasit P., Sittisombut N. Construction of infectious dengue 2 virus cDNA clones using high copy number plasmid. Journal of Virological Methods 2001, 92:71-82.
119. Tilgner, M.,Deas,T.S.,Shi,P.Y.,2005.The flavivirus-conservedpenta-nucleotide in the 30 stem-loop of the West Nile virus genome requires aspecificsequence and structure for RNA synthesis,but not for viral translation.Virology 331,375-386.
120. Suzuki R., de Borba L., Duarte dos Santos C.N., Mason P.W. Construction of an infectious cDNA clone for a Brazilian prototype strain of dengue virus type 1: characterization of a temperature-sensitive mutation in NS1. Virology 2007, 362:374-383.
121. Tomlinson, S.M.,Malmstrom,R.D.,Russo,A.,Mueller,N.,Pang,Y.P.,Watowich,S.J., 2009. Structure-based discovery of dengue virus protease in hibitors.Antiviral Research 82, 110-114.
122. Tilgner M., Deas T.S., Shi P.Y. The flavivirus-conserved penta-nucleotide in the 3' stem-loop of the West Nile virus genome requires a specific sequence and structure for RNA synthesis, but not for viral translation. Virology 2005, 331:375-386.
123. Varnavski, A.N.,Young,P.R.,Khromykh,A.A.,2000.Stable high-level expression of heterologous genes in vitro and invivo bynoncytopathic DNA-based Kunjin virus replicon vectors. Journal of Virology 74,4394-4403.
124. Tomlinson S.M., Malmstrom R.D., Russo A., Mueller N., Pang Y.P., Watowich S.J. Structure-based discovery of dengue virus protease inhibitors. Antiviral Research 2009, 82:110-114.
125. Villordo, S.M.,Gamarnik,A.V.,2009.Genome cyclization asstrategy for flavivirus RNA replication. Virus Research 139,230-239.
126. Varnavski A.N., Young P.R., Khromykh A.A. Stable high-level expression of heterologous genes in vitro and in vivo by noncytopathic DNA-based Kunjin virus replicon vectors. Journal of Virology 2000, 74:4394-4403.
127. Xie, X.,Wang,Q.Y.,Xu,H.Y.,Qing,M.,Kramer,L.,Yuan,Z.,Shi,P.Y.,2011.Inhibition of dengue virus bytargeting viral NS4B protein.Journal of Virology 85,11183-11195.
128. Villordo S.M., Gamarnik A.V. Genome cyclization as strategy for flavivirus RNA replication. Virus Research 2009, 139:230-239.
129. Yang, C.C.,Hsieh,Y.C.,Lee,S.J.,Wu,S.H.,Liao,C.L.,Tsao,C.H.,Chao,Y.S.,Chern,J.H.,Wu, C.P.,Yueh,A.,2011.Novel dengue virus-specificNS2B/NS3 protease in hibitor, BP2109,discovered by a high-throughput screening assay. Antimicrobial Agents and Chemotherapy 55,229-238.
130. Xie X., Wang Q.Y., Xu H.Y., Qing M., Kramer L., Yuan Z., Shi P.Y. Inhibition of dengue virus by targeting viral NS4B protein. Journal of Virology 2011, 85:11183-11195.
131. You, S.,Falgout,B.,Markof f,L.,Padmanabhan,R.,2001.In vitro RNA synthesis from exogenous dengue viral RNA templates requires long range interactions between 50- and 30-terminal regions that influenceRNA structure.The Journal of Biological Chemistry 276,15581-15591.
132. Yang C.C., Hsieh Y.C., Lee S.J., Wu S.H., Liao C.L., Tsao C.H., Chao Y.S., Chern J.H., Wu C.P., Yueh A. Novel dengue virus-specific NS2B/NS3 protease inhibitor, BP2109, discovered by a high-throughput screening assay. Antimicrobial Agents and Chemotherapy 2011, 55:229-238.
133. You, S.,Padmanabhan,R.,1999.Anovel In vitro replication system for Dengue virus. Initiation of RNA synthesis at the 30-end of exogenous viral RNA templates requires 50- and 30-terminal complementary sequence motifs of the viral RNA. The Journal of Biological Chemistry 274,33714-33722.
134. You S., Falgout B., Markoff L., Padmanabhan R. In vitro RNA synthesis from exogenous dengue viral RNA templates requires long range interactions between 5'- and 3'-terminal regions that influence RNA structure. The Journal of Biological Chemistry 2001, 276:15581-15591.
135. Yun, S.I.,Choi,Y.J.,Song,B.H.,Lee,Y.M.,2009.30 cis-acting elements that contribute to the competence and efficiencyof Japanese encephalitis virus genome replication: functional importance of sequence duplications,deletions, and substitutions. Journal of Virology 83,7909-7930.
136. You S., Padmanabhan R. A novel in vitro replication system for Dengue virus. Initiation of RNA synthesis at the 3'-end of exogenous viral RNA templates requires 5'- and 3'-terminal complementary sequence motifs of the viral RNA. The Journal of Biological Chemistry 1999, 274:33714-33722.
137. Zhang, J.H.,Chung,T.D.,Oldenburg,K.R.,1999.Asimple statistical parameter for use in evaluation and validation of high throughput screening assays.Journal of Bimolecular Screening 4, 67-73.
138. Yun S.I., Choi Y.J., Song B.H., Lee Y.M. 3' cis-acting elements that contribute to the competence and efficiency of Japanese encephalitis virus genome replication: functional importance of sequence duplications, deletions, and substitutions. Journal of Virology 2009, 83:7909-7930.
139. Qing, M.,Liu,W.,Yuan,Z.,Gu,F.,Shi,P.Y.,2010.Ahigh-throughput assay using dengue-1 virus-like particles for drug discovery.Antiviral Research 86,163- 171.
140. Zhang J.H., Chung T.D., Oldenburg K.R. A simple statistical parameter for use in evaluation and validation of high throughput screening assays. Journal of Bimolecular Screening 1999, 4:67-73.