Antigen-reactive T cell enrichment for direct, high-resolution analysis of the human naive and memory Th cell repertoire

Journal of Immunology

Volumn 190, Issue 8, 2013, Pages 3967-3976

  Bacher, Petra ^a   Schink, Christian ^a   Teutschbein, Janka ^b   Kniemeyer, Olaf ^b,c   Assenmacher, Mario ^a   Brakhage, Axel A ^b   Scheffold, Alexander ^d  

^a MILTENYI BIOTEC GMBH   (Germany)

^b FRIEDRICH SCHILLER UNIVERSITY JENA   (Germany)

^c JENA UNIVERSITY HOSPITAL   (Germany)

^d CHARITÉ UNIVERSITÄTSMEDIZIN BERLIN   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

CD4 ANTIGEN; CD40 LIGAND; CYTOKINE;

ANTIGEN SPECIFICITY; ARTICLE; CD154+ T LYMPHOCYTE; CD4+ T LYMPHOCYTE; CELLULAR IMMUNITY; CONTROLLED STUDY; CYTOKINE PRODUCTION; FLOW CYTOMETRY; HUMAN; HUMAN CELL; IMMUNOLOGICAL PROCEDURES; IMMUNOREACTIVITY; LYMPHOCYTE COUNT; MEMORY T LYMPHOCYTE; NONHUMAN; PRIORITY JOURNAL; T LYMPHOCYTE; T LYMPHOCYTE SUBPOPULATION; TARGET CELL;

ANTIGEN PRESENTATION; ANTIGEN-ANTIBODY REACTIONS; ANTIGEN-PRESENTING CELLS; ASPERGILLUS FUMIGATUS; CD4 LYMPHOCYTE COUNT; CELL DIFFERENTIATION; CELL LINE; CLONE CELLS; EPITOPES, T-LYMPHOCYTE; HUMANS; IMMUNOLOGIC MEMORY; T-LYMPHOCYTE SUBSETS; T-LYMPHOCYTES, HELPER-INDUCER;

EID: 84875985733     PISSN: 00221767     EISSN: 15506606     Source Type: Journal    
DOI: 10.4049/jimmunol.1202221     Document Type: Article

References (37)

1. Alanio, C., F. Lemaitre, H. K. Law, M. Hasan, and M. L. Albert. 2010. Enumeration of human antigen-specific naive CD8+ T cells reveals conserved precursor frequencies. Blood 115: 3718-3725.
2. Geiger, R., T. Duhen, A. Lanzavecchia, and F. Sallusto. 2009. Human naive and memory CD4+ T cell repertoires specific for naturally processed antigens analyzed using libraries of amplified T cells. J. Exp. Med. 206: 1525-1534.
3. Jenkins, M. K., H. H. Chu, J. B. McLachlan, and J. J. Moon. 2010. On the composition of the preimmune repertoire of T cells specific for peptide-major histocompatibility complex ligands. Annu. Rev. Immunol. 28: 275-294.
4. Bolton, D. L., and M. Roederer. 2009. Flow cytometry and the future of vaccine development. Expert Rev. Vaccines 8: 779-789.
5. Maecker, H. T. 2009. Multiparameter flow cytometry monitoring of T cell responses. Methods Mol. Biol. 485: 375-391.
6. Roederer, M., J. M. Brenchley, M. R. Betts, and S. C. De Rosa. 2004. Flow cytometric analysis of vaccine responses: how many colors are enough? Clin. Immunol. 110: 199-205.
7. Scheffold, A., and F. Kern. 2000. Recent developments in flow cytometry. J. Clin. Immunol. 20: 400-407.
8. Thiel, A., A. Scheffold, and A. Radbruch. 2004. Antigen-specific cytometry: new tools arrived! Clin. Immunol. 111: 155-161.
9. Angulo, R., and D. A. Fulcher. 1998. Measurement of Candida-specific blas-togenesis: comparison of carboxyfluorescein succinimidyl ester labelling of T cells, thymidine incorporation, and CD69 expression. Cytometry 34: 143-151.
10. Ho, W. Y., H. N. Nguyen, M. Wolfl, J. Kuball, and P. D. Greenberg. 2006. In vitro methods for generating CD8+ T-cell clones for immunotherapy from the naive repertoire. J. Immunol. Methods 310: 40-52.
11. Mannering, S. I., J. S. Morris, K. P. Jensen, A. W. Purcell, M. C. Honeyman, P. M. van Endert, and L. C. Harrison. 2003. A sensitive method for detecting proliferation of rare autoantigen-specific human T cells. J. Immunol. Methods 283: 173-183.
12. Novak, E. J., A. W. Liu, G. T. Nepom, and W. W. Kwok. 1999. MHC class II tetramers identify peptide-specific human CD4(+) T cells proliferating in response to influenza A antigen. J. Clin. Invest. 104: R63-R67.
13. Schneider, S., A. Bruns, B. Moewes, B. Holzknecht, G. Hausdorf, G. Riemekasten, A. Radbruch, F. Hiepe, and A. Thiel. 2002. Simultaneous cytometric analysis of (auto)antigen-reactive T and B cell proliferation. Immunobiology 206: 484-495.
14. Wolfl, M., J. Kuball, W. Y. Ho, H. Nguyen, T. J. Manley, M. Bleakley, and P. D. Greenberg. 2007. Activation-induced expression of CD137 permits detection, isolation, and expansion of the full repertoire of CD8+ T cells responding to antigen without requiring knowledge of epitope specificities. Blood 110: 201-210.
15. Barnes, E., S. M. Ward, V. O. Kasprowicz, G. Dusheiko, P. Klenerman, and M. Lucas. 2004. Ultra-sensitive class I tetramer analysis reveals previously undetectable populations of antiviral CD8+ T cells. Eur. J. Immunol. 34: 1570-1577.
16. Chu, H. H., J. J. Moon, K. Takada, M. Pepper, J. A. Molitor, T. W. Schacker, K. A. Hogquist, S. C. Jameson, and M. K. Jenkins. 2009. Positive selection optimizes the number and function of MHCII-restricted CD4+ T cell clones in the naive polyclonal repertoire. Proc. Natl. Acad. Sci. USA 106: 11241-11245.
17. Day, C. L., N. P. Seth, M. Lucas, H. Appel, L. Gauthier, G. M. Lauer, G. K. Robbins, Z. M. Szczepiorkowski, D. R. Casson, R. T. Chung, et al. 2003. Ex vivo analysis of human memory CD4 T cells specific for hepatitis C virus using MHC class II tetramers. J. Clin. Invest. 112: 831-842.
18. Kwok, W. W., V. Tan, L. Gillette, C. T. Littell, M. A. Soltis, R. B. LaFond, J. Yang, E. A. James, and J. H. DeLong. 2012. Frequency of epitope-specific naive CD4(+) T cells correlates with immunodominance in the human memory repertoire. J. Immunol. 188: 2537-2544.
19. Moon, J. J., H. H. Chu, M. Pepper, S. J. McSorley, S. C. Jameson, R. M. Kedl, and M. K. Jenkins. 2007. Naive CD4(+) T cell frequency varies for different epitopes and predicts repertoire diversity and response magnitude. Immunity 27: 203-213.
20. Obar, J. J., K. M. Khanna, and L. Lefrançois. 2008. Endogenous naive CD8+ T cell precursor frequency regulates primary and memory responses to infection. Immunity 28: 859-869.
21. Scriba, T. J., M. Purbhoo, C. L. Day, N. Robinson, S. Fidler, J. Fox, J. N. Weber, P. Klenerman, A. K. Sewell, and R. E. Phillips. 2005. Ultrasensitive detection and phenotyping of CD4+ T cells with optimized HLA class II tetramer staining. J. Immunol. 175: 6334-6343.
22. Wambre, E., E. A. James, and W. W. Kwok. 2012. Characterization of CD4+ T cell subsets in allergy. Curr. Opin. Immunol. 24: 700-706.
23. Nepom, G. T. 2012. MHC class II tetramers. J. Immunol. 188: 2477-2482.
24. Frentsch, M., O. Arbach, D. Kirchhoff, B. Moewes, M. Worm, M. Rothe, A. Scheffold, and A. Thiel. 2005. Direct access to CD4+ T cells specific for defined antigens according to CD154 expression. Nat. Med. 11: 1118-1124.
25. Chattopadhyay, P. K., J. Yu, and M. Roederer. 2005. A live-cell assay to detect antigen-specific CD4+ T cells with diverse cytokine profiles. Nat. Med. 11: 1113-1117.
26. Kirchhoff, D., M. Frentsch, P. Leclerk, D. Bumann, S. Rausch, S. Hartmann, A. Thiel, and A. Scheffold. 2007. Identification and isolation of murine antigen-reactive T cells according to CD154 expression. Eur. J. Immunol. 37: 2370-2377.
27. Weidner, G., C. d'Enfert, A. Koch, P. C. Mol, and A. A. Brakhage. 1998. Development of a homologous transformation system for the human pathogenic fungus Aspergillus fumigatus based on the pyrG gene encoding orotidine 59-monophosphate decarboxylase. Curr. Genet. 33: 378-385.
28. Macey, M. G. 2007. Flow Cytometry: Principles and Applications. Humana Press, Totowa, NJ.
29. Acosta-Rodriguez, E. V., L. Rivino, J. Geginat, D. Jarrossay, M. Gattorno, A. Lanzavecchia, F. Sallusto, and G. Napolitani. 2007. Surface phenotype and antigenic specificity of human interleukin 17-producing T helper memory cells. Nat. Immunol. 8: 639-646.
30. Brosterhus, H., S. Brings, H. Leyendeckers, R. A. Manz, S. Miltenyi, A. Radbruch, M. Assenmacher, and J. Schmitz. 1999. Enrichment and detection of live antigen-specific CD4(+) and CD8(+) T cells based on cytokine secretion. Eur. J. Immunol. 29: 4053-4059.
31. Dzionek, A., A. Fuchs, P. Schmidt, S. Cremer, M. Zysk, S. Miltenyi, D. W. Buck, and J. Schmitz. 2000. BDCA-2, BDCA-3, and BDCA-4: three markers for distinct subsets of dendritic cells in human peripheral blood. J. Immunol. 165: 6037-6046.
32. Leyendeckers, H., E. Voth, H. Schicha, N. Hunzelmann, P. Banga, and J. Schmitz. 2002. Frequent detection of thyroid peroxidase-specific IgG+ memory B cells in blood of patients with autoimmune thyroid disease. Eur. J. Immunol. 32: 3126-3132.
33. Zielinski, C. E., F. Mele, D. Aschenbrenner, D. Jarrossay, F. Ronchi, M. Gattorno, S. Monticelli, A. Lanzavecchia, and F. Sallusto. 2012. Pathogen-induced human TH17 cells produce IFN-γ or IL-10 and are regulated by IL-1β. Nature 484: 514-518.
34. Hoffmeister, B., F. Kiecker, L. Tesfa, H. D. Volk, L. J. Picker, and F. Kern. 2003. Mapping T cell epitopes by flow cytometry. Methods 29: 270-281.
35. Chu, H. H., J. J. Moon, A. C. Kruse, M. Pepper, and M. K. Jenkins. 2010. Negative selection and peptide chemistry determine the size of naive foreign peptide-MHC class II-specific CD4+ T cell populations. J. Immunol. 185: 4705-4713.
36. Jenkins, M. K., and J. J. Moon. 2012. The role of naive T cell precursor frequency and recruitment in dictating immune response magnitude. J. Immunol. 188: 4135-4140.
37. Newell, E. W., N. Sigal, S. C. Bendall, G. P. Nolan, and M. M. Davis. 2012. Cytometry by time-of-flight shows combinatorial cytokine expression and virus-specific cell niches within a continuum of CD8+ T cell phenotypes. Immunity 36: 142-152.