Analysis of telomere length and function in radiosensitive mouse and human cells in response to DNA-PKcs inhibition

Genome Integrity

Volumn 4, Issue 1, 2013, Pages

  Yasaei, Hemad ^a   Gozaly Chianea, Yaghoub ^a   Slijepcevic, Predrag ^a  

^a BRUNEL UNIVERSITY   (United Kingdom)

Author keywords

Artemis; DNA PKcs; Flow FISH; IC86621; Mouse lymphoma; Radiosensitivity; Telomere dysfunction; Telomere length

Indexed keywords

DNA PROTEIN KINASE; ENZYME INHIBITOR; IC 86621; PROTEIN KINASE; TELOMERASE; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; CATALYSIS; CHROMOSOME; CHROMOSOME FUSION; CONTROLLED STUDY; ENZYME INHIBITION; ENZYME SUBUNIT; FIBROBLAST; HUMAN; HUMAN CELL; LYMPHOMA CELL; MOUSE; NONHUMAN; PRIORITY JOURNAL; TELOMERE; TELOMERE HOMEOSTASIS; TELOMERE SHORTENING; TELOMERIC FUSION;

EID: 84875767503     PISSN: None     EISSN: 20419414     Source Type: Journal    
DOI: 10.1186/2041-9414-4-2     Document Type: Article

References (25)

1. Bailey SM, Brenneman MA, Halbrook J, Nickoloff JA, Ullrich RL, Goodwin EH. The kinase activity of DNA-PK is required to protect mammalian telomeres. DNA Repair (Amst) 2004, 3(3):225-233.
2. Frias C, Pampalona J, Genesca A, Tusell L. Telomere dysfunction and genome instability. Front Biosci 2012, 17:2181-2196. 10.2741/4044, 22652771.
3. de Lange T. Protection of mammalian telomeres. Oncogene 2002, 21(4):532-540. 10.1038/sj.onc.1205080, 11850778.
4. Goytisolo FA, Blasco MA. Many ways to telomere dysfunction: in vivo studies using mouse models. Oncogene 2002, 21(4):584-591. 10.1038/sj.onc.1205085, 11850783.
5. Palm W, de Lange T. How shelterin protects mammalian telomeres. Annu Rev Genet 2008, 42:301-334. 10.1146/annurev.genet.41.110306.130350, 18680434.
6. Slijepcevic P. DNA damage response, telomere maintenance and ageing in light of the integrative model. Mech Ageing Dev 2008, 129(1-2):11-16.
7. Bailey SM, Meyne J, Chen DJ, Kurimasa A, Li GC, Lehnert BE, Goodwin EH. DNA double-strand break repair proteins are required to cap the ends of mammalian chromosomes. Proc Natl Acad Sci U S A 1999, 96(26):14899-14904. 10.1073/pnas.96.26.14899, 24745, 10611310.
8. Espejel S, Franco S, Sgura A, Gae D, Bailey SM, Taccioli GE, Blasco MA. Functional interaction between DNA-PKcs and telomerase in telomere length maintenance. EMBO J 2002, 21(22):6275-6287. 10.1093/emboj/cdf593, 137185, 12426399.
9. Slijepcevic P, Al-Wahiby S. Telomere biology: integrating chromosomal end protection with DNA damage response. Chromosoma 2005, 114(4):275-285. 10.1007/s00412-005-0338-4, 15843951.
10. Yasaei H, Slijepcevic P. Defective Artemis causes mild telomere dysfunction. Genome Integr 2010, 1(1):3. 10.1186/2041-9414-1-3, 2907561, 20678254.
11. Ma Y, Pannicke U, Lu H, Niewolik D, Schwarz K, Lieber MR. The DNA-dependent protein kinase catalytic subunit phosphorylation sites in human Artemis. J Biol Chem 2005, 280(40):33839-33846. 10.1074/jbc.M507113200, 16093244.
12. Peddi P, Loftin CW, Dickey JS, Hair JM, Burns KJ, Aziz K, Francisco DC, Panayiotidis MI, Sedelnikova OA, Bonner WM. DNA-PKcs deficiency leads to persistence of oxidatively induced clustered DNA lesions in human tumor cells. Free Radic Biol Med 2010, 48(10):1435-1443. 10.1016/j.freeradbiomed.2010.02.033, 2901171, 20193758.
13. McIlrath J, Bouffler SD, Samper E, Cuthbert A, Wojcik A, Szumiel I, Bryant PE, Riches AC, Thompson A, Blasco MA. Telomere length abnormalities in mammalian radiosensitive cells. Cancer Res 2001, 61(3):912-915.
14. Wong HP, Slijepcevic P. Telomere length measurement in mouse chromosomes by a modified Q-FISH method. Cytogenet Genome Res 2004, 105(2-4):464-470.
15. Szumiel I. L5178Y sublines: a look back from 40 years. Part 2: response to ionizing radiation. Int J Radiat Biol 2005, 81(5):353-365. 10.1080/09553000500143534, 16076750.
16. Sprung CN, Davey DS, Goh SK, Radford IR, McKay MJ. Uncoupling of telomere length and radiosensitivity in mouse lymphoma cell lines of similar genetic background. Int J Radiat Biol 2007, 83(8):515-521. 10.1080/09553000701452270, 17613124.
17. Wlodek D, Hittelman WN. The repair of double-strand DNA breaks correlates with radiosensitivity of L5178Y-S and L5178Y-R cells. Radiat Res 1987, 112(1):146-155. 10.2307/3577085, 3659295.
18. Jaworska A, Szumiel I, De Angelis P, Olsen G, Reitan J. Evaluation of ionizing radiation sensitivity markers in a panel of lymphoid cell lines. Int J Radiat Biol 2001, 77(3):269-280. 10.1080/09553000010019638, 11258841.
19. Slijepcevic P. Telomeres and mechanisms of Robertsonian fusion. Chromosoma 1998, 107(2):136-140. 10.1007/s004120050289, 9601982.
20. Kashishian A, Douangpanya H, Clark D, Schlachter ST, Eary CT, Schiro JG, Huang H, Burgess LE, Kesicki EA, Halbrook J. DNA-dependent protein kinase inhibitors as drug candidates for the treatment of cancer. Mol Cancer Ther 2003, 2(12):1257-1264.
21. Cabuy E, Newton C, Roberts T, Newbold R, Slijepcevic P. Identification of subpopulations of cells with differing telomere lengths in mouse and human cell lines by flow FISH. Cytometry A 2004, 62(2):150-161.
22. Ma Y, Pannicke U, Schwarz K, Lieber MR. Hairpin opening and overhang processing by an Artemis/DNA-dependent protein kinase complex in nonhomologous end joining and V(D)J recombination. Cell 2002, 108(6):781-794. 10.1016/S0092-8674(02)00671-2, 11955432.
23. Riballo E, Kühne M, Rief N, Doherty A, Smith GC, Recio MJ, Reis C, Dahm K, Fricke A, Krempler A. A pathway of double-strand break rejoining dependent upon ATM, Artemis, and proteins locating to gamma-H2AX foci. Mol Cell 2004, 16(5):715-724. 10.1016/j.molcel.2004.10.029, 15574327.
24. Noordzij JG, Verkaik NS, van der Burg M, van Veelen LR, de Bruin-Versteeg S, Wiegant W, Vossen JM, Weemaes CM, de Groot R, Zdzienicka MZ. Radiosensitive SCID patients with Artemis gene mutations show a complete B-cell differentiation arrest at the pre-B-cell receptor checkpoint in bone marrow. Blood 2003, 101(4):1446-1452. 10.1182/blood-2002-01-0187, 12406895.
25. van der Burg M, van Dongen JJ, van Gent DC. DNA-PKcs deficiency in human: long predicted, finally found. Curr Opin Allergy Clin Immunol 2009, 9(6):503-509. 10.1097/ACI.0b013e3283327e41, 19823081.