Quantitation of fixative-induced morphologic and antigenic variation in mouse and human breast cancers

Laboratory Investigation

Volumn 93, Issue 4, 2013, Pages 480-497

  Cardiff, Robert D ^a,b   Hubbard, Neil E ^a   Engelberg, Jesse A ^a   Munn, Robert J ^a,b   Miller, Claramae H ^a,b   Walls, Judith E ^a   Chen, Jane Q ^a   Velásquez García, Héctor A ^a   Galvez, Jose J ^c   Bell, Katie J ^a   Beckett, Laurel A ^b   Li, Yue Ju ^b   Borowsky, Alexander D ^a,b  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

^c NATIONAL CANCER INSTITUTE   (United States)

Author keywords

estrogen receptor; Her2; IHC4 biomarker panel; immunohistochemistry; MIB1 (Ki67); progesterone receptor; quantitative image analysis

Indexed keywords

EPIDERMAL GROWTH FACTOR RECEPTOR 2; FIXATIVE; FORMALDEHYDE; KI 67 ANTIGEN; TUMOR ANTIGEN;

ALGORITHM; ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ANTIGENIC VARIATION; ARTICLE; BREAST CANCER; CANCER MORPHOLOGY; CONTROLLED STUDY; HUMAN; HUMAN TISSUE; IMMUNOGENICITY; IMMUNOHISTOCHEMISTRY; MOUSE; NONHUMAN; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REPRODUCIBILITY;

EID: 84875707939     PISSN: 00236837     EISSN: 15300307     Source Type: Journal    
DOI: 10.1038/labinvest.2013.10     Document Type: Article

References (42)

1. Dunstan RW, Wharton Jr KA, Quigley C, et al. The use of immunohistochemistry for biomarker assessment-can it compete with other technologies? Toxicol Pathol 2011;39:988-1002.
2. Kothmaier H, Rohrer D, Stacher E, et al. Comparison of formalin-free tissue fixatives: a proteomic study testing their application for routine pathology and research. Arch Pathol Lab Med 2011;135:744-752.
3. NHS. Evidence review: non-formalin fixatives. In: National Health Service Purchasing and Supply Agency CfE-bP editor. Reading, UK, Vol. CEP090919 (2009), pp1-36.
4. Belief V, Boissiere F, Bibeau F, et al. Proteomic analysis of RCL2 paraffin-embedded tissues. J Cell Mol Med 2008;12:2027-2036.
5. Boon ME, Kok LP. Theory and practice of combining coagulant fixation and microwave histoprocessing. Biotech Histochem 2008;83:261-277.
6. Chung JY, Braunschweig T, Williams R, et al. Factors in tissue handling and processing that impact RNA obtained from formalin-fixed, paraffin-embedded tissue. J Histochem Cytochem 2008;56:1033-1042.
7. Cox ML, Schray CL, Luster CN, et al. Assessment of fixatives, fixation, and tissue processing on morphology and RNA integrity. Exp Mol Pathol 2006;80:183-191.
8. Delfour C, Roger P, Bret C, et al. RCL2, a new fixative, preserves morphology and nucleic acid integrity in paraffin-embedded breast carcinoma and microdissected breast tumor cells. J Mol Diagn 2006;8:157-169.
9. Dotti I, Bonin S, Basili G, et al. Effects of formalin, methacarn, and fineFIX fixatives on RNA preservation. Diagn Mol Pathol 2010;19: 112-122.
10. Espina V, Mueller C, Edmiston K, et al. Tissue is alive: new technologies are needed to address the problems of protein biomarker pre-analytical variability. Proteomics Clin Appl 2009;3:874-882.
11. Gazziero A, Guzzardo V, Aldighieri E, et al. Morphological quality and nucleic acid preservation in cytopathology. J Clin Pathol 2009;62: 429-434.
12. Giannella C, Zito FA, Colonna F, et al. Comparison of formalin, ethanol, and Histochoice fixation on the PCR amplification from paraffin-embedded breast cancer tissue. Eur J Clin Chem Clin Biochem 1997;35:633-635.
13. Gomez-Fernandez C, Daneshbod Y, Nassiri M, et al. Immunohistochemically determined estrogen receptor phenotype remains stable in recurrent and metastatic breast cancer. Am J Clin Pathol 2008;130:879-882.
14. Jensen UB, Owens DM, Pedersen S, et al. Zinc fixation preserves flow cytometry scatter and fluorescence parameters and allows simultaneous analysis of DNA content and synthesis, and intracellular and surface epitopes. Cytometry A 2010;77: 798-804.
15. Lykidis D, Van Noorden S, Armstrong A, et al. Novel zinc-based fixative for high quality DNA, RNA and protein analysis. Nucleic Acids Res 2007;35:e85.
16. Marchionni L, Wilson RF, Marinopoulos SS, et al. Impact of gene expression profiling tests on breast cancer outcomes. Evid Rep Technol Assess 2007;160:1-105.
17. Mikaelian I, Nanney LB, Parman KS, et al. Antibodies that label paraffin-embedded mouse tissues: a collaborative endeavor. Toxicol Pathol 2004;32:181-191.
18. Mook S, Schmidt MK, Weigelt B, et al. The 70-gene prognosis signature predicts early metastasis in breast cancer patients between 55 and 70 years of age. Ann Oncol 2009;21:717-722.
19. Nassiri M, Ramos S, Zohourian H, et al. Preservation of biomolecules in breast cancer tissue by a formalin-free histology system. BMC Clin Pathol 2008;8:1.
20. O'Leary TJ, Fowler CB, Evers DL, et al. Protein fixation and antigen retrieval: chemical studies. Biotech Histochem 2009;84: 217-221.
21. Otali D, Stockard CR, Oelschlager DK, et al. Combined effects of formalin fixation and tissue processing on immunorecognition. Biotech Histochem 2009;84:223-247.
22. Paizs M, Engelhardt JI, Siklos L. Quantitative assessment of relative changes of immunohistochemical staining by light microscopy in specified anatomical regions. J Microsc 2009;234: 103-112.
23. Prento P, Lyon H. Commercial formalin substitutes for histopathology. Biotech Histochem 1997;72:273-282.
24. Srinivasan M, Sedmak D, Jewell S. Effect of fixatives and tissue processing on the content and integrity of nucleic acids. Am J Pathol 2002;161:1961-1971.
25. Stanta G, Mucelli SP, Petrera F, et al. A novel fixative improves opportunities of nucleic acids and proteomic analysis in human archive's tissues. Diagn Mol Pathol 2006;15:115-123.
26. Titford ME, Horenstein MG. Histomorphologic assessment of formalin substitute fixatives for diagnostic surgical pathology. Arch Pathol Lab Med 2005;129:502-506.
27. Vincek V, Nassiri M, Nadji M, et al. A tissue fixative that protects macromolecules (DNA, RNA, and protein) and histomorphology in clinical samples. Lab Invest 2003;83:1427-1435.
28. Xie R, Chung JY, Ylaya K, et al. Factors influencing the degradation of archival formalin-fixed paraffin-embedded tissue sections. J Histochem Cytochem 2011;59:356-365.
29. Viertler C, Groelz D, Gündisch S, et al. A new technology for stabilization of biomolecules in tissues for combined histological and molecular analyses. J Mol Diagn 2012;14:458-466.
30. Cuzick J, Dowsett M, Pineda S, et al. Prognostic value of a combined estrogen receptor, progesterone receptor, Ki-67, and human epidermal growth factor receptor 2 immunohistochemical score and comparison with the Genomic Health recurrence score in early breast cancer. J Clin Oncol 2011;29:4273-4278.
31. Chan SR, Vermi W, Luo J, et al. STAT1-deficient mice spontaneously develop estrogen receptor alpha-positive luminal mammary carcinomas. Breast Cancer Res 2012;14:R16.
32. Borowsky AD, Namba R, Young LJ, et al. Syngeneic mouse mammary carcinoma cell lines: two closely related cell lines with divergent metastatic behavior. Clin Exp Metastasis 2005;22:47-59.
33. Siegel PM, Ryan ED, Cardiff RD, et al. Elevated expression of activated forms of Neu/ErbB-2 and ErbB-3 are involved in the induction of mammary tumors in transgenic mice: implications for human breast cancer. EMBO J 1999;18:2149-2164.
34. Lloyd MC, Allam-Nandyala P, Purohit CN, et al. Using image analysis as a tool for assessment of prognostic and predictive biomarkers for breast cancer: How reliable is it? J Pathol Inform 2010;1:29.
35. Mulrane L, Rexhepaj E, Penney S, et al. Automated image analysis in histopathology: a valuable tool in medical diagnostics. Expert Rev Mol Diagn 2008;8:707-725.
36. Potts SJ, Krueger JS, Landis ND, et al. Evaluating tumor heterogeneity in immunohistochemistry-stained breast cancer tissue. Lab Invest 2012;92:1342-1357.
37. Noe M, Kunz G, Herbertz M, et al. The cyclic pattern of the immunocytochemical expression of oestrogen and progesterone receptors in human myometrial and endometrial layers: characterization of the endometrial- subendometrial unit. Hum Reprod 1999;14:190-197.
38. Lehmann EL, D'Abrera HJM. Nonparametrics: Statistical Methods Based On Ranks. Rev. 1st edn (, New York, 2006) xvi463p.
39. Fox CH, Johnson FB, Whiting J, et al. Formaldehyde fixation. J Histochem Cytochem 1985;33:845-853.
40. Allred DC, Carlson RW, Berry DA, et al. NCCN Task Force Report: estrogen receptor and progesterone receptor testing in breast cancer by immunohistochemistry. J Natl Compr Canc Netw 2009;7(Suppl 6): S1-S21, quiz S22-23.
41. McClelland RA, Finlay P, Walker KJ, et al. Automated quantitation of immunocytochemically localized estrogen receptors in human breast cancer. Cancer Res 1990;50:3545-3550.
42. Nadji M. Quantitative immunohistochemistry of estrogen receptor in breast cancer: 'much ado about nothing!'. Appl Immunohistochem Mol Morphol 2008;16:105-107.