Telomere length analysis of human mesenchymal stem cells by quantitative PCR

Gene

Volumn 519, Issue 2, 2013, Pages 348-355

  Samsonraj, Rebekah M ^a,b   Raghunath, Michael ^b,c   Hui, James H ^c   Ling, Ling ^a   Nurcombe, Victor ^a   Cool, Simon M ^a,c  

^a INSTITUTE OF MEDICAL BIOLOGY   (Singapore)

^b NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

^c NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

Author keywords

Cell senescence; Human mesenchymal stem cells; Quantitative PCR; Telomerase; Telomere length; Telomeres

Indexed keywords

ARTICLE; CELL PROLIFERATION; CLONOGENESIS; CONTROLLED STUDY; HUMAN; HUMAN CELL; MESENCHYMAL STEM CELL; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; TELOMERE; TELOMERE SHORTENING;

BLOTTING, SOUTHERN; CELL PROLIFERATION; CELL SURVIVAL; CHROMOSOMES, HUMAN; DNA; GENE DOSAGE; GENE EXPRESSION; HUMANS; IN SITU HYBRIDIZATION, FLUORESCENCE; MESENCHYMAL STROMAL CELLS; REAL-TIME POLYMERASE CHAIN REACTION; TELOMERASE; TELOMERE; TELOMERE SHORTENING;

EUKARYOTA;

EID: 84875628848     PISSN: 03781119     EISSN: 18790038     Source Type: Journal    
DOI: 10.1016/j.gene.2013.01.039     Document Type: Article

References (30)

1. Baxter M.A., Wynn R.F., Jowitt S.N., Wraith J.E., Fairbairn L.J., Bellantuono I. Study of telomere length reveals rapid aging of human marrow stromal cells following in vitro expansion. Stem Cells 2004, 22:675-682.
2. Bernardo M.E., et al. Human bone marrow-derived mesenchymal stem cells do not undergo transformation after long-term in vitro culture and do not exhibit telomere maintenance mechanisms. Cancer Res. 2007, 67:9142-9149.
3. Canela A., Klatt P., Blasco M. Telomere length analysis. Biological Aging 2007, 45-72. Humana Press. T. Tollefsbol (Ed.).
4. Caplan A.I. Mesenchymal stem-cells. J. Orthop. Res. 1991, 9:641-650.
5. Caplan A.I., Bruder S.P. Mesenchymal stem cells: building blocks for molecular medicine in the 21st century. Trends Mol. Med. 2001, 7:259-264.
6. Cawthon R.M. Telomere measurement by quantitative PCR. Nucleic Acids Res. 2002, 30.
7. Cawthon R.M. Telomere length measurement by a novel monochrome multiplex quantitative PCR method. Nucleic Acids Res. 2009, 37.
8. Chiu C.P., et al. Differential expression of telomerase activity in hematopoietic progenitors from adult human bone marrow. Stem Cells 1996, 14:239-248.
9. da Costa C.E.T., et al. Differences in telomerase expression by the CD1a(+) cells in Langerhans cell histiocytosis reflect the diverse clinical presentation of the disease. J. Pathol. 2007, 212:188-197.
10. Daniel M., Peek G.W., Tollefsbol T.O. Regulation of the human catalytic subunit of telomerase (hTERT). Gene 2012, 498:135-146.
11. Dominici M., et al. Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement. Cytotherapy 2006, 8:315-317.
12. Forsyth N.R., Wright W.E., Shay J.W. Telomerase and differentiation in multicellular organisms: turn it off, turn it on, and turn it off again. Differentiation 2002, 69:188-197.
13. Freisinger E., et al. Characterization of hematopoietic potential of mesenchymal stem cells. J. Cell. Physiol. 2010, 225:888-897.
14. Greider C.W., Blackburn E.H. Identification of a specific telomere terminal transferase activity in Tetrahymena extracts. Cell 1985, 43:405-413.
15. Guillot P.V., Gotherstrom C., Chan J., Kurata H., Fisk N.M. Human first-trimester fetal MSC express pluripotency markers and grow faster and have longer telomeres than adult MSC. Stem Cells 2007, 25:646-654.
16. Helledie T., et al. Heparan sulfate enhances the self-renewal and therapeutic potential of mesenchymal stem cells from human adult bone marrow. Stem Cells Dev. 2012, 21:1897-1910.
17. Kang S.K., Putnam L., Dufour J., Ylostalo J., Jung J.S., Bunnell B.A. Expression of telomerase extends the lifespan and enhances osteogenic differentiation of adipose tissue-derived stromal cells. Stem Cells 2004, 22:1356-1372.
18. Kobune M., et al. Telomerized human multipotent mesenchymal cells can differentiate into hematopoietic and cobblestone area-supporting cells. Exp. Hematol. 2003, 31:715-722.
19. Livak K.J., Schmittgen T.D. Analysis of relative gene expression data using real-time quantitative PCR and the 2(T) (-Delta Delta C) method. Methods 2001, 25:402-408.
20. O'Callaghan N.J., Fenech M. A quantitative PCR method for measuring absolute telomere length. Biol. Proced. Online 2011, 13.
21. Okamoto T., et al. Clonal heterogeneity in differentiation potential of immortalized human mesenchymal stem cells. Biochem. Biophys. Res. Commun. 2002, 295:354-361.
22. Parsch D., Brummendorf T.H., Richter W., Fellenberg J. Replicative aging of human articular chondrocytes during ex vivo expansion. Arthritis Rheum. 2002, 46:2911-2916.
23. Parsch D., Fellenberg J., Brummendorf T.H., Eschlbeck A.M., Richter W. Telomere length and telomerase activity during expansion and differentiation of human mesenchymal stem cells and chondrocytes. J. Mol. Med. 2004, 82:49-55.
24. Pittenger M.F., et al. Multilineage potential of adult human mesenchymal stem cells. Science 1999, 284:143-147.
25. Poole J.C., Andrews L.G., Tollefsbol T.O. Activity, function, and gene regulation of the catalytic subunit of telomerase (hTERT). Gene 2001, 269:1-12.
26. Rider D.A., et al. Autocrine fibroblast growth factor 2 increases the multipotentiality of human adipose-derived mesenchymal stem cells. Stem Cells 2008, 26:1598-1608.
27. Shi S.T., et al. Bone formation by human postnatal bone marrow stromal stem cells is enhanced by telomerase expression. Nat. Biotechnol. 2002, 20:587-591.
28. Thomson J.A., et al. Embryonic stem cell lines derived from human blastocysts. Science 1998, 282:1145-1147.
29. Yap L.Y., et al. Defining a threshold surface density of vitronectin for the stable expansion of human embryonic stem cells. Tissue Eng Part C Methods 2011, 17:193-207.
30. Zimmermann S., Voss M., Kaiser S., Kapp U., Waller C.F., Martens U.M. Lack of telomerase activity in human mesenchymal stem cells. Leukemia 2003, 17:1146-1149.