Detection of toxins A/B and isolation of Clostridium difficile and Clostridium perfringens from dogs in Minas Gerais, Brazil

Brazilian Journal of Microbiology

Volumn 44, Issue 1, 2013, Pages 133-137

  Silva, Rodrigo Otávio Silveira ^a   Santos, Renata Lara Resende ^a   Pires, Prhiscylla Sadanã ^a   Carlos Pereira, Luiz ^a   Trindade Pereira, Silvia ^a   Carvalho Duarte, Marina ^a   de Assis, Ronnie Antunes ^b   Lobato, Francisco Carlos Faria ^a  

^a FEDERAL UNIVERSITY OF MINAS GERAIS   (Brazil)

^b Laboratorio Nacional Agropecuário Minas Gerais   (Brazil)

Author keywords

Canine; Colitis; Enteritis; Nosocomial diarrhea; Small animals

Indexed keywords

ANIMALIA; CANIS FAMILIARIS; CLOSTRIDIUM DIFFICILE; CLOSTRIDIUM PERFRINGENS;

EID: 84875578490     PISSN: 15178382     EISSN: 16784405     Source Type: Journal    
DOI: 10.1590/S1517-83822013005000008     Document Type: Article

References (44)

1. Alvarez-Perez S, Blanco JL, Bouza E, Alba P, Gibert X, Maldonado J, Garcia ME (2009) Prevalence of Clostridium difficile in diarrhoeic and non-diarrhoeic piglets. Vet Microbiol 137:302-305.
2. Arroyo LG, Staempfli H, Weese JS (2007) Molecular analysis of Clostridium difficile isolates recovered from horses with diarrhea. Vet Microbiol 120:179-183.
3. Avbersek J, Janezic S, Pate M, Rupnik M, Zidaric V, Logar K, Vengust M, Zemljic M, Pirs T, Ocepek M (2009) Diversity of Clostridium difficile in pigs and other animals in Slovenia. Anaerobe 15:252-255.
4. Bakri MM, Brown DJ, Butcher JP, Sutherland AD (2009) Clostridium difficile in ready-to-eat salads, Scotland. Emerg Infect Dis 15:817-818.
5. Barbut F, Decré D, Lalande V, Burghoffer B, Noussair L, Gigandon A, Espinasse F, Raskine L, Robert J, Mangeol A, Branger C, Petit JC (2005) Clinical features of Clostridium difficile-associated diarrhoea due to binary toxin (actinspecific ADP ribosyltransferase)-producing strains. J Med Microbiol 54:181-185.
6. Baums CG, Schotte U, Amtsberg G, Goethe R (2004) Diagnostic multiplex PCR for toxin genotype of Clostridium perfringens isolates. Vet Mic 100:11-16.
7. Baverud V (2002) Clostridium difficile infections in animals with special reference to the horse. Areview. VetQ24:203-219.
8. Berry AP, Levett PN (1986) Chronic diarrhea in dogs associated with Clostridium difficile infection. Vet Rec 118:102-103.
9. Buggy BP, Wilson KH, Fekety R (1983) Comparison of methods for recovery of Clostridium difficile from an environmental surface. J Clin Microbiol 18:348-352.
10. Chouicha N, Marks SL (2006) Evaluation of five enzyme immunoassays compared with the cytotoxicity assay for diagnosis of Clostridium difficile-associated diarrhea in dogs. J Vet Diagn Invest 18:182-188.
11. Clooten J, Kruth S, Arroyo L, Weese JS (2008) Prevalence and risk factors for Clostridium difficile colonization in dogs and cats hospitalized in an intensive care unit. Vet Microbiol 129:209-214.
12. Delmeé M (2001) Laboratory diagnosis of Clostridium difficile disease. Clin Microbiol Infect 7:411-416.
13. Eriksen J, Zenner D, Anderson SR, Grant K, Kumar D (2010) Clostridium perfringens in London, July 2009: Two weddings and an outbreak. Euro Surveill 15:19598.
14. Fedorko DP, Williams EC (1997) Use of cycloserine-cefoxitinfructose agar and L-proline-aminopeptidase (PRO Discs) in the rapid identification of Clostridium difficile. J Clinic Microbiol 35:1258-1259.
15. Gonçalves C, Decré D, Barbut F, Burghoffer B, Petit JC (2004) Prevalence and characterization of a binary toxin (actinspecific ADP-ribosyltransferase) from Clostridium difficile. J Clinic Microbiol 42:1933-1999.
16. Herholz C, Miserez R, Nicolet J, Frey J, Popoff M, Gibert M, Gerber H, Straub R (1999) Prevalence of beta2-toxigenic Clostridium perfringens in horses with intestinal disorders. J Clinic Microbiol 37:358-361.
17. Keel MK, Songer JG (2006) The comparative pathology of Clostridium difficile-associated disease. Vet Pathol 43:225-240.
18. Kruth SA, Prescott JF, Welch MK, Brodsky MH (1989) Nosocomial diarrhea associated with enterotoxigenic Clostridium perfringens infection in dogs. J Am Vet Med Assoc 195:331-334.
19. Kyne L, Warny M, Qamar A, Kelly CP (2000) Asymptomatic carriage of Clostridium difficile and serum levels of IgG antibody against toxin A. N Engl J Med 342:390-397.
20. Lefebvre SL, Arroyo LG, Weese JS (2006) Epidemic Clostridium difficile strain in hospital visitation dog. Emerg Infect Dis 12:1036-1037.
21. Marks SL, Kather EJ, Kass PH, Melli AC (2002) Genotypic and phenotypic characterization of Clostridium perfringens and Clostridium difficile in diarrheic and healthy dogs. J Vet Intern Med 15:533-540.
22. Norman KN, Harvey RB, Scott HM, Hume ME, Andrews K, Brawley AD (2009) Varied prevalence of Clostridium difficile in an integrated swine operation. Anaerobe 15:256-260.
23. Persson S, Torpdahl M, Olsen KEP (2008) New multiplex PCR method for the detection of Clostridium difficile toxin A (tcdA) and toxin B (tcdB) and the binary toxin (cdtA/cdtB) genes applied to a Danish strain collection. Clinic Microbiol Infect 14:1057-1064.
24. Rodriguez-Palacios A, Staempfli HR, Duffield T, Weese JS (2007) Clostridium difficile in retail ground meat, Canada. Emerg Infect Dis 13:485-487.
25. Rudmann DG, Vandereide SL (2003) Necrotizing enterotyphlocolitis in dogs treated with a potent antimuscarinic. Vet Pathol 40:710-713.
26. Sasaki J, Goryo M, Asahina M, Makara M, Shishido S, Okada K (1999) Hemorrhagic enteritis associated with Clostridium perfringens type A in a dog. J Vet Med Sci 61:175-177.
27. Schwan C, Stecher B, Tzivelekidis T, van Ham M, Rohde M, Hardt WD, Wehland J, Aktories K (2009) Clostridium difficile toxin CDT induces formation of microtubule-based protrusions and increases adherence of bacteria. PLoS Pathog 5:e1000626.
28. Silva ROS, Salvarani FM, Cruz Junior ECC, Pires PS, Santos RLR, Assis RA, Guedes RMC, Lobato FCF (2011) Detection of enterotoxin A and cytotoxin B, and isolation of Clostridium difficile in piglets in Minas Gerais, Brazil. Ciência Rural 41:1430-1435.
29. Silva ROS, Salvarani FM, Assis RA, Martins NRS, Pires PS, Lobato FCF (2009) Antimicrobial susceptibility of Clostridium perfringens strains isolated from broiler chickens. Braz J Microbiol 40:261-263.
30. Silva ROS, Guedes RMC, Lobato FCF (2013a) Clostridium difficile infection: Main features and occurrence in domestic species in Brazil. Ciência Rural 43:73-80.
31. Silva ROS, Ribeiro MG, Palhares MS, Borges AS, Maranhão RPA, Silva MX, Lucas TM, Lobato FCF (2013b) Detection of A/B toxin and isolation of Clostridium difficile and C. perfringens from foals. Equine Vet J, doi 10.1111/evj.12046.
32. Silva ROS, Lobato FCF, Pires PS, Gabardo MP, Paladino ES, Guedes RMC (2013c) Neonatal diarrhea in piglets associated with cpb-2 positive Clostridium perfringens. Braz J Vet Pathol 6:94-98.
33. Siqueira FF, Almeida MO, Barroca TM, Horta CC, Carmo AO, Silva RO, Pires PS, Lobato FC, Kalapothakis E (2012) Characterization of polymorphisms and isoforms of the Clostridium perfringens phospholipase C gene (plc) reveals high genetic diversity. Vet Microbiol 159:397-405.
34. Songer JG, Trinh HT, Killgore GE, Thompson AD, McDonald LC, Limbago BM (2009) Clostridium difficile in retail meat products, USA, 2007. Emerg Infect Dis 15:819-821.
35. Songer JG, Uzal FA (2005) Clostridial enteric infections in pigs. J Vet Diagn Invest 17:528-536.
36. Songer JG (2010) Clostridia as agents of zoonotic disease. Vet Microbiol 140:399-404.
37. Struble AL, Tang YJ, Kass PH, Gumerlock PH, Madewell BR, Silva Jr J (1994) Fecal shedding of Clostridium difficile in dogs: a period prevalence survey in a veterinary medical teaching hospital. J Vet Diagn Invest 6:342-347.
38. Stubbs S, Rupnik M, Gibert M, Brazier J, Duerden B, Popoff M (2000) Production of actin-specific ADP-ribosyltransferase (binary toxin) by strains of Clostridium difficile. FEMS Microbiol Lett 186:307-312.
39. Vieira AAS, Guedes RMC, Salvarani FM, Silva ROS, Assis RA, Lobato FCF (2008) Genotipagem de Clostridium perfringens isolados de leitões diarréicos. Arq Instit Biol 75:513-516.
40. Voth DE, Ballard JD (2005) Clostridium difficile toxins: mechanism of action and role in disease. Clin Microbiol 18:247-263.
41. Waggett BE, McGorum BC, Wernery U, Shaw DJ, Pirie RS (2010) Prevalence of Clostridium perfringens in faeces and ileal contents from grass sickness affected horses: comparisons with 3 control populations. Equine Vet J 42:494-499.
42. Weese JS, Armstrong J (2003) Outbreak of Clostridium difficileassociated disease in a small animal veterinary teaching hospital. J Vet Int Med 17:813-816.
43. Weese JS, Staempfli HR, Prescott JF, Kruth SA, Greenwood SJ, Weese HE (2001) The roles of Clostridium difficile and enterotoxigenic Clostridium perfringens in diarrhea in dogs. J Vet Intern Med 15:374-378.
44. Yaeger MJ, Kinyon JM, Songer JG (2007) A prospective, case control study evaluating the association between Clostridium difficile toxins in the colon of neonatal swine and gross and microscopic lesions. J Vet Diagn Invest 19:52-59.