Mutant methionyl-tRNA synthetase from bacteria enables site-selective N-terminal labeling of proteins expressed in mammalian cells

Proceedings of the National Academy of Sciences of the United States of America

Volumn 110, Issue 13, 2013, Pages 4992-4997

  Ngo, John T ^a   Schuman, Erin M ^b   Tirrell, David A ^a  

^a California Institute of Technology   (United States)

^b MAX PLANCK INSTITUTE FOR BRAIN RESEARCH   (Germany)

Author keywords

Protein engineering; Protein synthesis; Proteomics; Translational profiling

Indexed keywords

BACTERIAL ENZYME; BACTERIAL RNA; METHIONINE TRANSFER RNA LIGASE; METHIONYL AMINOPEPTIDASE; PROTEIN P53; TRANSFER RNA;

AMINO TERMINAL SEQUENCE; AMINOACYLATION; ARTICLE; CAENORHABDITIS ELEGANS; CELL CYCLE; CELL CYCLE ARREST; CELL LYSATE; CONTROLLED STUDY; DNA REPLICATION; ESCHERICHIA COLI; FLUORESCENCE IMAGING; HETEROLOGOUS EXPRESSION; MAMMAL CELL; MUTANT; NONHUMAN; PRIORITY JOURNAL; PROTEIN LOCALIZATION; PROTEIN SYNTHESIS; TANDEM MASS SPECTROMETRY; TRANSLATION INITIATION;

ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; HEK293 CELLS; HUMANS; LEUCINE; METHIONINE-TRNA LIGASE; PEPTIDE CHAIN INITIATION, TRANSLATIONAL; RNA, TRANSFER, MET;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; MAMMALIA;

EID: 84875523875     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.1216375110     Document Type: Article

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