Qualification of a homogeneous cell-based neonatal Fc receptor (FcRn) binding assay and its application to studies on Fc functionality of IgG-based therapeutics

Journal of Immunological Methods

Volumn 390, Issue 1-2, 2013, Pages 81-91

  Mathur, Abhishek ^a   Arora, Taruna ^a   Liu, Ling ^a   Crouse Zeineddini, Jill ^a   Mukku, Venkat ^a  

^a AMGEN INC   (United States)

Author keywords

Assay qualification; Bioassay; Fc; FcRn; Flow cytometry; Immunoglobulin

Indexed keywords

FC RECEPTOR; FLUORESCENT DYE; IMMUNOGLOBULIN FC FRAGMENT; IMMUNOGLOBULIN G; PROTEIN NEONATAL FC RECEPTOR; UNCLASSIFIED DRUG;

ACCURACY; ANALYTIC METHOD; ANIMAL CELL; ANTIBODY COMBINING SITE; ARTICLE; BINDING ASSAY; BINDING COMPETITION; CONTROLLED STUDY; DRUG RECEPTOR BINDING; FLOW CYTOMETRY; FLUORESCENCE; HEAT STRESS; HUMAN; HUMAN CELL; LIGHT STRESS; MOLECULAR STABILITY; MUTANT; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PROTEIN INTERACTION;

AMINO ACIDS; ANALYSIS OF VARIANCE; ANIMALS; BINDING SITES; BINDING, COMPETITIVE; CHO CELLS; CRICETINAE; CRICETULUS; FLOW CYTOMETRY; HEK293 CELLS; HISTOCOMPATIBILITY ANTIGENS CLASS I; HUMANS; HYDROGEN-ION CONCENTRATION; IMMUNOGLOBULIN FC FRAGMENTS; IMMUNOGLOBULIN G; PROTEIN BINDING; RECEPTORS, FC;

EID: 84875270732     PISSN: 00221759     EISSN: 18727905     Source Type: Journal    
DOI: 10.1016/j.jim.2013.01.011     Document Type: Article

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