The detection and quantification of ethyl glucuronide in placental tissue and placental perfusate by headspace solid-phase microextraction coupled with gas chromatography-mass spectrometry

Journal of Population Therapeutics and Clinical Pharmacology

Volumn 19, Issue 3, 2012, Pages

  Matlow, Jeremy N ^a,b   Aleksa, Katarina ^a,c   Lubetsky, Angelika ^a   Koren, Gideon ^a,b  

^a HOSPITAL FOR SICK CHILDREN   (Canada)

^b UNIVERSITY OF TORONTO   (Canada)

^c UNIVERSITY OF WATERLOO   (Canada)

Author keywords

Biomarker; Ethyl glucuronide; Ex vivo placental perfusion model; Gas chromatography; Headspace solid phase microextraction; Placenta

Indexed keywords

ETHYL GLUCURONIDE;

ARTICLE; CELL VIABILITY; DERIVATIZATION; FEMALE; FETUS; HUMAN; HUMAN TISSUE; LIMIT OF DETECTION; MASS FRAGMENTOGRAPHY; NORMAL HUMAN; PLACENTA; REGRESSION ANALYSIS; REPRODUCIBILITY; SOLID PHASE EXTRACTION; SOLID PHASE MICROEXTRACTION; SPECTRAL SENSITIVITY;

ALCOHOL DRINKING; BIOLOGICAL MARKERS; FEMALE; GAS CHROMATOGRAPHY-MASS SPECTROMETRY; GLUCURONATES; HUMANS; LIMIT OF DETECTION; MATERNAL-FETAL EXCHANGE; PERFUSION; PLACENTA; PREGNANCY; SOLID PHASE MICROEXTRACTION; SUBSTANCE ABUSE DETECTION;

EID: 84875225780     PISSN: 17106222     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (27)

1. Foti RS, Fischer MB. Assessment of UDP-glucuronosyltransferase catalyzed formation of ethyl glucuronide in human liver microsomes and recombinant UGTs. Forensic Sci Int 2005;153:109-116.
2. Halter CC, Dresen S, Auwaerter V, Wurst FM, Weinmann W. Kinetics in serum and urine excretion of ethyl sulfate and ethyl glucuronide after medium dose ethanol intake. Int J Legal Med 2008;122:123-128.
3. Hoiseth G, Bernard JP, Karinen R, et al. A pharmacokinetic study of ethyl glucuronide in blood and urine: applications to forensic toxicology. Forensic Sci Int 2007;172:119-124.
4. Hoiseth G, Morini L, Polettini A, Christophersen A, Morland J. Blood kinetics of ethyl glucuronide and ethyl sulfate in heavy drinkers during alcohol detoxification. Forensic Sci Int 2009;188:52-26.
5. Borucki K, Schreiner R, Dierkes J, et al. Detection of recent ethanol intake with new markers: comparison of fatty acid ethyl esters in serum and of ethyl glucuronide and the ratio of 5-hydroxytryptophol to 5- hydroxyindole acetic acid in urine. Alcohol Clin Exp Res 2005;29:781-787.
6. Gareri J, Lynn H, Handley M, Rao C, Koren G. Prevalence of fetal ethanol exposure in a regional population-based sample by meconium analysis of fatty acid ethyl esters. Ther Drug Monit 2008;30:239-245.
7. Backdash A, Burger P, Goecke TW, et al. Quantification of fatty acid ethyl esters (FAEE) and ethyl glucuronide (EtG) in meconium from newborns for detection of alcohol abuse in a maternal health evaluation study. Anal Bioanal Chem 2010;396:2469-2477.
8. Morini L, Groppi A, Marchei E, et al. Population baseline of meconium ethyl glucuronide and ethyl sulfate concentrations in newborns of nondrinking women in 2 Mediterranean cohorts. Ther Drug Monit 2010;32:359-363.
9. Pichini S, Marchei E, Magnarelli F, et al. Assessment of prenatal exposure to ethanol by meconium analysis: results of an Italian multicenter study. Alcohol Clin Exp Res 2012;36:417-424.
10. Tarcomnicu I, van Nuijs AL, Aerts K, et al. Ethyl glucuronide determination in meconium and hair by hydrophilic interaction liquid chromatography-tandem mass spectrometry. Forensic Sci Int 2010;196:121-127.
11. Morini L, Falcon M, Pichini S, et al. Ethyl- glucuronide and ethyl-sulfate in placental and fetal tissues by liquid chromatography coupled with tandem mass spectrometry. Anal Biochem 2011;418:30-36.
12. Hutson JR, Garcia-Bournissen F, Davis A, Koren G. The human placental perfusion model: a systematic review and development of a model to predict in vivo transfer of therapeutic drugs. Clin Pharmacol Ther 2011;90:67-76.
13. Janda I, Alt A. Improvement of ethyl glucuronide determination in human urine and serum samples by solid-phase extraction. J Chromatogr B Biomed Sci Appl 2001;758:229-234.
14. Schmitt G, Aderjan R, Keller T, Wu M. Ethyl glucuronide: an unusual ethanol metabolite in humans. Synthesis, analytical data, and determination in serum and urine. J Anal Toxicol 1995;19:91-94.
15. Shen BH, Yan H, Zhao H, Zhuo XY. [Determination of ethyl glucuronide in blood by GC-MS/MS]. Fa Yi Xue Za Zhi 2009;25:431-433.
16. Pragst F. Application of solid-phase microextraction in analytical toxicology. Anal Bioanal Chem 2007;388:1393-1414.
17. Miller RK, Wier PJ, Maulik D, di Sant'Agnese PA. Human placenta in vitro: characterization during 12 h of dual perfusion. Contrib Gynecol Obstet 1985;13:77-84.
18. Derewlany LO, Leeder JS, Kumar R, et al. The transport of digoxin across the perfused human placental lobule. J Pharmacol Exp Ther 1991;256:1107-1011.
19. Pollex EK, Anger G, Hutson J, Koren G, Piquette-Miller M. Breast cancer resistance protein (BCRP)-mediated glyburide transport: effect of the C421A/Q141K BCRP-single- nucleotide polymorphism. Drug Metab Dispos 2010;38:740-744.
20. Kerekes I, Yegles M, Grimm U, Wennig R. Ethyl glucuronide determination: head hair versus non-head hair. Alcohol Alcohol 2009;44:62-66.
21. Yegles M, Labarthe A, Auwarter V, et al. Comparison of ethyl glucuronide and fatty acid ethyl ester concentrations in hair of alcoholics, social drinkers and teetotallers. Forensic Sci Int 2004;145:167-173.
22. Agius R, Nadulski T, Kahl HG, et al. Validation of a headspace solid-phase microextraction-GC-MS/MS for the determination of ethyl glucuronide in hair according to forensic guidelines. Forensic Sci Int 2010;196:3-9.
23. Hoiseth G, Yttredal B, Karinen R, et al. Ethyl glucuronide concentrations in oral fluid, blood, and urine after volunteers drank 0.5 and 1.0 g/kg doses of ethanol. J Anal Toxicol 2010;34:319-324.
24. Hoiseth G, Morini L, Polettini A, et al. Serum/whole blood concentration ratio for ethylglucuronide and ethyl sulfate. J Anal Toxicol 2009;33:208-211.
25. Aleksa K, Walasek P, Fulga N, et al. Simultaneous detection of seventeen drugs of abuse and metabolite in hair using solid phase micro extraction (SPME) with GC/MS. Forensic Sci Int 2012;218:31-36.
26. Pawliszyn J, Pawliszyn B, Pawliszyn M. Solid phase microextraction (SPME). Chem Educator 1997;2:1-7.
27. Jurado C, Soriano T, Gimenez MP, Menendez M. Diagnosis of chronic alcohol consumption. Hair analysis for ethyl-glucuronide. Forensic Sci Int 2004;145:161-166.