Knockdown of Myostatin Expression by RNAi Enhances Muscle Growth in Transgenic Sheep

PLoS ONE

Volumn 8, Issue 3, 2013, Pages

  Hu, Shengwei ^a   Ni, Wei ^a   Sai, Wujiafu ^a   Zi, Ha ^a   Qiao, Jun ^a   Wang, Pengyang ^a   Sheng, Jinliang ^a   Chen, Chuangfu ^a  

^a SHIHEZI UNIVERSITY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

MYOSTATIN; SHORT HAIRPIN RNA;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; CELL CLONE; CELL NUCLEUS TRANSPLANTATION; CONTROLLED STUDY; FIBROBLAST; GENE CASSETTE; GENE EXPRESSION; MALE; MUSCLE CELL; MUSCLE GROWTH; MUSCLE TISSUE; NONHUMAN; PHENOTYPE; PROTEIN EXPRESSION; PROTEIN RNA BINDING; PROTEIN TARGETING; REGULATORY MECHANISM; RNA INTERFERENCE; SOMATIC CELL; TRANSGENIC SHEEP; WEIGHT GAIN;

ANIMALS; ANIMALS, GENETICALLY MODIFIED; BASE SEQUENCE; BODY WEIGHT; CLONING, ORGANISM; GENE EXPRESSION; GENE KNOCKDOWN TECHNIQUES; MUSCLE, SKELETAL; MYOGENIC REGULATORY FACTORS; MYOSTATIN; RNA INTERFERENCE; RNA, SMALL INTERFERING; SHEEP, DOMESTIC;

EID: 84875201948     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0058521     Document Type: Article

References (32)

1. McPherron AC, Lawler AM, Lee SJ, (1997) Regulation of skeletal muscle mass in mice by a new TGF-ß superfamily member. Nature 387: 83-90.
2. McPherron AC, Lee SJ, (1997) Double muscling in cattle due to mutations in the myostatin gene. Proc Natl Acad Sci USA 94: 12457-12461.
3. Kambadur R, Sharma M, Smith TPL, Bass JJ, (1997) Mutations in myostatin (GDF8) in double-muscled Belgian Blue and Piedmontese cattle. Genome Res 7: 910-915.
4. Grobet L, Martin LJR, Poncelet D, Pirottin D, Brouwers B, et al. (1997) A deletion in the bovine myostatin gene causes the double-muscled phenotype in cattle. Nat Genet 17: 71-74.
5. Clop A, Marcq F, Takeda H, Pirottin D, Tordoir X, et al. (2006) A mutation creating a potential illegitimate microRNA target site in the myostatin gene affects muscularity in sheep. Nat Genet 38: 813-818.
6. Mosher DS, Quignon P, Bustamante CD, Sutter NB, Mellersh CS, et al. (2007) A mutation in the myostatin gene increases muscle mass and enhances racing performance in heterozygote dogs. PLoS Genet 3: 779-786.
7. Schuelke M, Wagner KR, Stolz LE, Hübner C, Riebel T, et al. (2004) Myostatin mutation associated with gross muscle hypertrophy in a child. N Engl J Med 350: 2682-2688.
8. Meister G, Tuschl T, (2004) Mechanisms of gene silencing by double-stranded RNA. Nature 431: 343-349.
9. Hemann MT, Fridman JS, Zilfou JT, Hernando E, Paddison PJ, et al. (2003) An epi-allelic series of p53 hypomorphs created by stable RNAi produces distinct tumor phenotypes in vivo. Nat Genet 33: 396-400.
10. Wang PY, Jiang JJ, Li N, Sheng JL, Ren Y, et al. (2010) Transgenic mouse model integrating siRNA targeting the foot and mouth disease virus. Antivir Res 87: 265-268.
11. Lee CY, Hu SY, Gong HY, Chen MHC, Lu JK, et al. (2009) Suppression of myostatin with vector-based RNA interference causes a double-muscle effect in transgenic zebrafish. Biochem Biophys Res Commun 387: 766-771.
12. Acosta J, Carpio Y, Borroto I, Gonzalez O, Estrada MP, (2005) Myostatin gene silenced by RNAi show a zebrafish giant phenotype. J Biotechnol 119: 324-331.
13. Hu S, Ni W, Sai W, Zhang H, Cao X, et al. (2011) Sleeping Beauty-mediated knockdown of sheep myostatin by RNA interference. Biotechnol Lett 33: 1949-1953.
14. Hu S, Ni W, Hazi W, Zhang H, Zhang N, et al. (2011) Cloning and functional analysis of sheep U6 promoters. Anim Biotechnol 22: 170-174.
15. Sai WJF, Peng XR, Li XC, An ZX, Zheng YM, et al. (2008) Effects of ovine sperm extract on the development competence of homogeneous and heterogeneous embryos reconstructed by nuclear transfer. Acta Vet Zootech Sinica 39: 1343-1348.
16. Hu S, Ni W, Chen C, Sai W, Hazi W, et al. (2012) Comparison between the effects of valproic acid and trichostatin a on in vitro development of sheep somatic cell nuclear transfer embryos. Journal of Animal and Veterinary Advances 11: 1868-1872.
17. Haidet AM, Rizo L, Handy C, Umapathi P, Eagle A, et al. (2008) Long-term enhancement of skeletal muscle mass and strength by single gene administration of myostatin inhibitors. Proc Natl Acad Sci USA 105: 4318-4322.
18. Lee SJ, McPherron AC, (2001) Regulation of myostatin activity and muscle growth. Proc Natl Acad Sci USA 98: 9306-9311.
19. Lee SJ, (2007) Quadrupling muscle mass in mice by targeting TGF-ß signaling pathways. PLoS ONE 2: e789.
20. Lee SJ, (2008) Genetic analysis of the role of proteolysis in the activation of latent myostatin. PLoS ONE 3: e1628.
21. Bellinge RH, Liberles DA, Iaschi SP, O'Brien PA, Tay GK, (2005) Myostatin and its implications on animal breeding: A review. Anim Genet 36: 1-6.
22. Arthur PF, (1995) Double muscling in cattle: A review. Aust J Agric Res 46: 1493-1515.
23. Tessanne K, Golding MC, Long CR, Peoples MD, Hannon G, et al. (2012) Production of transgenic calves expressing an shRNA targeting myostatin. Mol Reprod Dev 79: 176-185.
24. Mehta AK, Majumdar SS, Alam P, Gulati N, Brahmachari V, (2009) Epigenetic regulation of cytomegalovirus major immediateearly promoter activity in transgenic mice. Gene 428: 20-24.
25. Kong Q, Wu M, Huan Y, Zhang L, Liu H, et al. (2009) Transgene expression is associated with copy number and cytomegalovirus promoter methylation in transgenic pigs. PloS ONE 4: e6679.
26. Duan B, Cheng L, Gao Y, Yin FX, Su GH, et al. (2012) Silencing of fat-1 transgene expression in sheep may result from hypermethylation of its driven cytomegalovirus (CMV) promoter. Theriogenology 78: 793-802.
27. Guo Z, Wang L, Eisensmith R, Woo S, (1996) Evaluation of promoter strength for hepatic gene expression in vivo following adenovirus-mediated gene transfer. Gene Ther 3: 802-810.
28. Toth M, Lichtenberg U, Doerfler W, (1989) Genomic sequencing reveals a 5-methylcytosine-free domain in active promoters and the spreading of preimposed methylation patterns. Proc Natl Acad Sci USA 86: 3728-32.
29. Schnieke AE, Kind AJ, Ritchie WA, Mycock K, Scott AR, et al. (1997) Human factor IX transgenic sheep produced by transfer of nuclei from transfected fetal fibroblasts. Science 278: 2130-2133.
30. Fletcher CJ, Roberts CT, Hartwich KM, Walker SK, McMillen IC, (2007) Somatic cell nuclear transfer in the sheep induces placental defects that likely precede fetal demise. Reproduction 133: 243-255.
31. Nishi M, Yasue A, Nishimatu S, Nohno T, Yamaoka T, et al. (2002) A missense mutant myostatin causes hyperplasia without hypertrophy in the mouse muscle. Biochem Biophys Res Commun 293: 247-251.
32. Zhu X, Hadhazy M, Wehling M, Tidball JG, McNally EM, (2000) Dominant negative myostatin produces hypertrophy without hyperplasia in muscle. FEBS Lett 474: 71-75.