Sleeping Beauty-mediated knockdown of sheep myostatin by RNA interference

Biotechnology Letters

Volumn 33, Issue 10, 2011, Pages 1949-1953

  Hu, Shengwei ^a   Ni, Wei ^a   Sai, Wujiafu ^a   Zhang, Hui ^a   Cao, Xudong ^a   Qiao, Jun ^a   Sheng, Jinliang ^a   Guo, Fei ^a   Chen, Chuangfu ^a  

^a SHIHEZI UNIVERSITY   (China)

Author keywords

Myostatin; RNA interference; Sheep fetal fibroblasts; Sleeping Beauty

Indexed keywords

ANIMAL CLONING; ANIMAL MUSCLES; DONOR CELLS; GENE KNOCK DOWN; LIVESTOCK PRODUCTION; MYOSTATIN; NEGATIVE REGULATORS; POTENTIAL TOOL; REAL-TIME PCR; RNA INTERFERENCE; SKELETAL MUSCLE GROWTH; SLEEPING BEAUTY; TRANSFECTED CELLS; TRANSPOSON SYSTEM;

AGRICULTURE; ANIMALS; CELL CULTURE; CLONE CELLS; CLONING; FIBROBLASTS; GENE EXPRESSION; MUSCLE; POLYMERASE CHAIN REACTION; RNA; SLEEP RESEARCH;

WOOL;

MYOSTATIN; SMALL INTERFERING RNA; TRANSPOSASE;

ANIMAL; ARTICLE; CELL CULTURE; FIBROBLAST; GENE SILENCING; GENETIC TRANSFECTION; GENETICS; METABOLISM; METHODOLOGY; PHYSIOLOGY; REAL TIME POLYMERASE CHAIN REACTION; RNA INTERFERENCE; SHEEP;

ANIMALS; CELLS, CULTURED; FIBROBLASTS; GENE KNOCKDOWN TECHNIQUES; MYOSTATIN; REAL-TIME POLYMERASE CHAIN REACTION; RNA INTERFERENCE; RNA, SMALL INTERFERING; SHEEP; TRANSFECTION; TRANSPOSASES;

ANIMALIA; OVIS ARIES;

EID: 80052801947     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-011-0667-8     Document Type: Article

References (18)

1. Belur LR, Frandsen JL, Dupuy AJ, Ingbar DH, Largaespada DA, Hackett PB, McIvor RS (2003) Gene insertion and long-term expression in lung mediated by the Sleeping Beauty transposon system. Mol Ther 8: 501-507.
2. Chen ZJ, Kren BT, Wong PY, Low WC, Steer CJ (2005) Sleeping beauty-mediated down-regulation of huntingtin expression by RNA interference. Biochem Biophys Res Commun 329: 646-652.
3. Devon RS, Porteous DJ, Brookes AJ (1995) Splinkerettes-improved vectorettes for greater efficiency in PCR walking. Nucleic Acids Res 23: 1644-1645.
4. Dupuy AJ, Fritz S, Largaespada DA (2001) Transposition and gene disruption in the male germline of the mouse. Genesis 30: 82-88.
5. Heggestad AD, Notterpek L, Fletcher BS (2004) Transposon-based RNAi delivery system for generating knockdown cell lines. Biochem Biophys Res Commun 316: 643-650.
6. Hemann MT, Fridman JS, Zilfou JT, Hernando E, Paddison PJ, Cordon-Cardo C, Hannon GJ, Lowe SW (2003) An epi-allelic series of p53 hypomorphs created by stable RNAi produces distinct tumor phenotypes in vivo. Nat Genet 33: 396-400.
7. Hu SW, Ni W, Hazi W, Zhang H, Zhang N, Meng R, Chen CF (2011) Cloning and functional analysis of sheep U6 promoters. Anim Biotechnol (accept).
8. Ivics Z, Hackett PB, Plasterk RH, Izsvák Z (1997) Molecular reconstruction of sleeping beauty, a Tc1-like transposon from fish, and its transposition in human cells. Cell 91: 501-510.
9. Izsvák Z, Ivics Z, Plasterk RH (2000) Sleeping beauty, a wide hostrange transposon vector for genetic transformation in vertebrates. J Mol Biol 302: 93-102.
10. Kambadur R, Sharma M, Smith TPL, Bass JJ (1997) Mutations in myostatin (GDF8) in double-muscled Belgian blue and Piedmontese cattle. Genome Res 7: 910-915.
11. Kren BT, Gosh SS, Linehan CL, Roy-Chowdhury N, Hackett PB, Roy-Chowdhury J, Steer CJ (2003) Hepatocyte-targeted delivery of sleeping beauty mediates efficient transposition in vivo. Gene Ther Mol Biol 7: 231-240.
12. Lee CY, Hu SY, Gong HY, Chen MHC, Lu JK, Wu JL (2009) Suppression of myostatin with vector-based RNA interference causes a double-muscle effect in transgenic zebrafish. Biochem Biophys Res Commun 387: 766-771.
13. Livek KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCt method. Methods 25: 402-408.
14. McPherron AC, Lee SJ (1997) Double muscling in cattle due to mutations in the myostatin gene. Proc Natl Acad Sci USA 94: 12457-12461.
15. McPherron AC, Lawler AM, Lee SJ (1997) Regulation of skeletal muscle mass in mice by a new TGF-β superfamily member. Nature 387: 83-90.
16. Mosher DS, Quignon P, Bustamante CD, Sutter NB, Mellersh CS, Parker HG, Ostrander EA (2007) A mutation in the myostatin gene increases muscle mass and enhances racing performance in heterozygote dogs. PLoS Genet 3: 779-786.
17. Vigdal TJ, Kaufman CD, Izsvák Z, Voytas DF, Ivics Z (2002) Common physical properties of DNA affecting target site selection of Sleeping Beauty and other Tc1/mariner transposable elements. J Mol Biol 323: 441-452.
18. Wang PY, Jiang JJ, Li N, Sheng JL, Ren Y, Chen CF, Guo ZR (2010) Transgenic mouse model integrating siRNA targeting the foot and mouth disease virus. Antivir Res 87: 265-268.