A convenient cellular assay for the identification of the molecular target of ergosterol biosynthesis inhibitors and quantification of their effects on total ergosterol biosynthesis

Steroids

Volumn 78, Issue 5, 2013, Pages 483-493

  Müller, Christoph ^a   Staudacher, Verena ^a   Krauss, Jürgen ^a   Giera, Martin ^b   Bracher, Franz ^a  

^a UNIVERSITY OF MUNICH   (Germany)

^b LEIDEN UNIVERSITY MEDICAL CENTER   (Netherlands)

Author keywords

Antifungals; Candida glabrata; Enzyme inhibitors; GC MS analysis; Isotope labeling; Liquid liquid micro extraction

Indexed keywords

14 METHYLERGOSTA 8,24(28) DIEN 3BETA,6ALPHA DIOL; ACETIC ACID; AGENTS AFFECTING LIPID METABOLISM; CARBON 13; CLOTRIMAZOLE; DR 180; ERGOSTEROL; ERGOSTEROL BIOSYNTHESIS INHIBITOR; JK 250; LANOSTEROL; TRIDEMORPH; UNCLASSIFIED DRUG;

ARTICLE; CELL LYSATE; DRUG POTENCY; DRUG SCREENING; DRUG TARGETING; MASS FRAGMENTOGRAPHY; QUALITATIVE ANALYSIS; QUANTITATIVE ANALYSIS; STEROL SYNTHESIS;

ACETATES; CANDIDA GLABRATA; DRUG EVALUATION, PRECLINICAL; ENZYME INHIBITORS; ERGOSTEROL; GAS CHROMATOGRAPHY-MASS SPECTROMETRY; INHIBITORY CONCENTRATION 50; LIQUID PHASE MICROEXTRACTION; METHYLTRANSFERASES; SACCHAROMYCES CEREVISIAE; YARROWIA;

CANDIDA GLABRATA; FUNGI;

EID: 84875157919     PISSN: 0039128X     EISSN: 18785867     Source Type: Journal    
DOI: 10.1016/j.steroids.2013.02.006     Document Type: Article

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