Minor variant detection in amplicons using 454 massive parallel pyrosequencing: Experiences and considerations for successful applications

BioTechniques

Volumn 51, Issue 3, 2011, Pages 167-177

  Vandenbroucke, Ina ^a   van Marck, Herwig ^a   Verhasselt, Peter ^a   Thys, Kim ^a   Mostmans, Wendy ^a   Dumont, Stéphanie ^a   van Eygen, Veerle ^a   Coen, Katrien ^a   Tuefferd, Marianne ^a   Aerssens, Jeroen ^a  

^a Tibotec Virco Virology   (Belgium)

Author keywords

454 pyrosequencing; DNA polymerase; Minor variant detection; Sequencing error; Ultra deep sequencing

Indexed keywords

DNA POLYMERASE;

AMPLICON; ARTICLE; DNA SEQUENCE; GENE AMPLIFICATION; POLYMERASE CHAIN REACTION; PYROSEQUENCING;

BASE SEQUENCE; GENETIC VARIATION; HIGH-THROUGHPUT NUCLEOTIDE SEQUENCING; HUMANS; POLYMERASE CHAIN REACTION; QUALITY CONTROL; REFERENCE STANDARDS; SEQUENCE ANALYSIS, DNA;

EID: 80053458464     PISSN: 07366205     EISSN: 19409818     Source Type: Journal    
DOI: 10.2144/000113733     Document Type: Article

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