Aliphatic 1H, 13C and 15N chemical shift assignments of dihydrofolate reductase from the psychropiezophile Moritella profunda in complex with NADP+ and folate

Biomolecular NMR Assignments

Volumn 7, Issue 1, 2013, Pages 61-64

  Loveridge, E Joel ^a,d   Matthews, Stella M ^a   Williams, Christopher ^b   Whittaker, Sara B M ^c   Günther, Ulrich L ^c   Evans, Rhiannon M ^a   Dawson, William M ^a   Crump, Matthew P ^b   Allemann, Rudolf K ^a  

^a CARDIFF UNIVERSITY   (United Kingdom)

^b UNIVERSITY OF BRISTOL   (United Kingdom)

^c UNIVERSITY OF BIRMINGHAM   (United Kingdom)

^d SWANSEA UNIVERSITY   (United Kingdom)

Author keywords

Conformational heterogeneity; Dihydrofolate reductase; Moritella profunda

Indexed keywords

DIHYDROFOLATE REDUCTASE; FOLIC ACID; NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE;

ARTICLE; CHEMISTRY; ENZYMOLOGY; METABOLISM; MORITELLA; NUCLEAR MAGNETIC RESONANCE;

FOLIC ACID; MORITELLA; NADP; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; TETRAHYDROFOLATE DEHYDROGENASE;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; MORITELLA; MORITELLA PROFUNDA;

EID: 84874997943     PISSN: 18742718     EISSN: 1874270X     Source Type: Journal    
DOI: 10.1007/s12104-012-9378-x     Document Type: Article

References (11)

1. Bhabha G, Tuttle L, Martinez-Yamout MA, Wright PE (2011) Identification of endogenous ligands bound to bacterially expressed human and E. coli dihydrofolate reductase by 2D NMR. FEBS Lett 585(22):3528-3532
2. Delaglio F, Grzesiek S, Vuister GW, Zhu G, Pfeifer J, Bax A (1995) NMRPipe: a multidimensional spectral processing system based on UNIX pipes. J Biomol NMR 6(3):277-293
3. Evans RM, Behiry EM, Tey L-H, Guo J, Loveridge EJ, Allemann RK (2010) Catalysis by dihydrofolate reductase from the psychropiezophile Moritella profunda. ChemBioChem 11(14):2010-2017
4. Hata K, Kono R, Fujisawa M, Kitahara R, Kamatari YO, Akasaka K, Xu Y (2004) High pressure NMR study of dihydrofolate reductase from a deep-sea bacterium. Cell Mol Biol 50(4):311-316
5. Hay S, Evans RM, Levy C, Loveridge EJ, Wang X, Leys D, Allemann RK, Scrutton NS (2009) Are the catalytic properties of enzymes from piezophilic organisms pressure adapted? ChemBioChem 10(14):2348-2353
6. Loveridge EJ, Tey L-H, Behiry EM, Dawson WM, Evans RM, Whittaker SB-M, Günther UL, Williams C, Crump MP, Allemann RK (2011) The role of large-scale motions in catalysis by dihydrofolate reductase. J Am Chem Soc 133(50):20561-20570
7. Osborne MJ, Venkitakrishnan RP, Dyson HJ, Wright PE (2003) Diagnostic chemical shift markers for loop conformation and substrate and cofactor binding in dihydrofolate reductase complexes. Prot Sci 12(10):2230-2238
8. Sawaya MR, Kraut J (1997) Loop and subdomain movements in the mechanism of Escherichia coli dihydrofolate reductase: crystallographic evidence. Biochemistry 36(3):586-603
9. Vranken WF, Boucher W, Stevens TJ, Fogh RH, Pajon A, Llinas M, Ulrich EL, Markley JL, Ionides J, Laue ED (2005) The CCPN data model for NMR spectroscopy: development of a software pipeline. Proteins 59(4):687-696
10. Xu Y, Nogi Y, Kato C, Liang Z, Rüger HJ, De Kegel D, Glansdorff N (2003a) Moritella profunda sp. nov. and Moritella abyssi sp. nov., two psychropiezophilic organisms isolated from deep Atlantic sediments. Int J Syst Evol Microbiol 53(2):533-538
11. Xu Y, Feller G, Gerday C, Glansdorff N (2003b) Moritella Cold-Active Dihydrofolate Reductase: are There Natural Limits to Optimization of Catalytic Efficiency at Low Temperature? J Bacteriol 185(18):5519-5526