Loss of the Keratin Cytoskeleton Is Not Sufficient to Induce Epithelial Mesenchymal Transition in a Novel KRAS Driven Sporadic Lung Cancer Mouse Model

PLoS ONE

Volumn 8, Issue 3, 2013, Pages

  König, Katharina ^a   Meder, Lydia ^a   Kröger, Cornelia ^b   Diehl, Linda ^c   Florin, Alexandra ^a   Rommerscheidt Fuss, Ursula ^a   Kahl, Philip ^a   Wardelmann, Eva ^a   Magin, Thomas M ^d   Buettner, Reinhard ^a   Heukamp, Lukas C ^a  

^a UNIVERSITY OF COLOGNE   (Germany)

^b WHITEHEAD INSTITUTE FOR BIOMEDICAL RESEARCH   (United States)

^c UNIVERSITY OF BONN   (Germany)

^d UNIVERSITY OF LEIPZIG   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

BETA CATENIN; CRE RECOMBINASE; KERATIN; TRANSCRIPTION FACTOR SLUG; TRANSCRIPTION FACTOR SNAIL; UVOMORULIN;

ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; CANCER INVASION; CELL ADHESION; CELL PROLIFERATION; CONTROLLED STUDY; CYTOSKELETON; EMBRYO; EPITHELIAL MESENCHYMAL TRANSITION; HUMAN; HUMAN CELL; HUMAN TISSUE; IMMUNOFLUORESCENCE; LUNG ADENOCARCINOMA; MORPHOLOGY; MOUSE; NONHUMAN; ONCOGENE K RAS; PHENOTYPE; PROMOTER REGION; PROTEIN ANALYSIS; PROTEIN DEPLETION; PROTEIN EXPRESSION; PROTEIN FUNCTION; SIGNAL TRANSDUCTION; STOP CODON; TUMOR VOLUME;

ANIMALS; BIOLOGICAL MARKERS; CARCINOMA, NON-SMALL-CELL LUNG; CELL LINE; DISEASE MODELS, ANIMAL; EPITHELIAL-MESENCHYMAL TRANSITION; GENE EXPRESSION REGULATION, NEOPLASTIC; GENE ORDER; GENE TARGETING; GENES, RAS; HUMANS; KERATINS; LUNG NEOPLASMS; MICE; MICE, TRANSGENIC; SMALL CELL LUNG CARCINOMA;

EID: 84874910004     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0057996     Document Type: Article

References (31)

1. Polyak K, Weinberg RA, (2009) Transitions between epithelial and mesenchymal states: acquisition of malignant and stem cell traits. Nat Rev Cancer 9: 265-273.
2. Mani SA, Guo W, Liao MJ, Eaton EN, Ayyanan A, et al. (2008) The epithelial-mesenchymal transition generates cells with properties of stem cells. Cell 133: 704-715.
3. Shintani Y, Okimura A, Sato K, Nakagiri T, Kadota Y, et al. (2011) Epithelial to mesenchymal transition is a determinant of sensitivity to chemoradiotherapy in non-small cell lung cancer. Ann Thorac Surg 92: 1794-1804; discussion 1804.
4. Meuwissen R, Linn SC, van der Valk M, Mooi WJ, Berns A, (2001) Mouse model for lung tumorigenesis through Cre/lox controlled sporadic activation of the K-Ras oncogene. Oncogene 20: 6551-6558.
5. Meuwissen R, Linn SC, Linnoila RI, Zevenhoven J, Mooi WJ, et al. (2003) Induction of small cell lung cancer by somatic inactivation of both Trp53 and Rb1 in a conditional mouse model. Cancer Cell 4: 181-189.
6. Zakowski MF, Ladanyi M, Kris MG, (2006) Memorial Sloan-Kettering Cancer Center Lung Cancer OncoGenome G (2006) EGFR mutations in small-cell lung cancers in patients who have never smoked. N Engl J Med 355: 213-215.
7. Sequist LV, Waltman BA, Dias-Santagata D, Digumarthy S, Turke AB, et al. (2011) Genotypic and histological evolution of lung cancers acquiring resistance to EGFR inhibitors. Sci Transl Med 3: 75ra26.
8. Peifer M, Fernandez-Cuesta L, Sos ML, George J, Seidel D, et al. (2012) Integrative genome analyses identify key somatic driver mutations of small-cell lung cancer. Nat Genet.
9. Seltmann K, Roth W, Kroger C, Loschke F, Lederer M, et al. (2012) Keratins Mediate Localization of Hemidesmosomes and Repress Cell Motility. J Invest Dermatol.
10. Kroger C, Vijayaraj P, Reuter U, Windoffer R, Simmons D, et al. (2011) Placental vasculogenesis is regulated by keratin-mediated hyperoxia in murine decidual tissues. Am J Pathol 178: 1578-1590.
11. Kuner R, Muley T, Meister M, Ruschhaupt M, Buness A, et al. (2009) Global gene expression analysis reveals specific patterns of cell junctions in non-small cell lung cancer subtypes. Lung Cancer 63: 32-38.
12. Stahl S, Sacher T, Bechtold A, Protzer U, Ganss R, et al. (2009) Tumor agonist peptides break tolerance and elicit effective CTL responses in an inducible mouse model of hepatocellular carcinoma. Immunol Lett 123: 31-37.
13. Heukamp LC, van der Burg SH, Drijfhout JW, Melief CJ, Taylor-Papadimitriou J, et al. (2001) Identification of three non-VNTR MUC1-derived HLA-A*0201-restricted T-cell epitopes that induce protective anti-tumor immunity in HLA-A2/K(b)-transgenic mice. Int J Cancer 91: 385-392.
14. Gazdar AF, Carney DN, Russell EK, Sims HL, Baylin SB, et al. (1980) Establishment of continuous, clonable cultures of small-cell carcinoma of lung which have amine precursor uptake and decarboxylation cell properties. Cancer Res 40: 3502-3507.
15. Naito S, Sueishi K, Hattori F, Tanaka K, (1980) Immunological analysis of plasminogen activators from cultured human cancer cells. Virchows Arch A Pathol Anat Histol 387: 251-257.
16. de Leij L, Postmus PE, Buys CH, Elema JD, Ramaekers F, et al. (1985) Characterization of three new variant type cell lines derived from small cell carcinoma of the lung. Cancer Res 45: 6024-6033.
17. Verbeeck MA, Elands JP, de Leij LF, Buys CH, Carney DN, et al. (1992) Expression of the vasopressin and gastrin-releasing peptide genes in small cell lung carcinoma cell lines. Pathobiology 60: 136-142.
18. Heukamp LC, Schroder DW, Plassmann D, Homann J, Buttner R, (2003) Marked clinical and histologic improvement in a patient with type-1 Gaucher's disease following long-term glucocerebroside substitution. A case report and review of current diagnosis and management. Pathol Res Pract 199: 159-163.
19. Peitz M, Jager R, Patsch C, Jager A, Egert A, et al. (2007) Enhanced purification of cell-permeant Cre and germline transmission after transduction into mouse embryonic stem cells. Genesis 45: 508-517.
20. Johnson L, Mercer K, Greenbaum D, Bronson RT, Crowley D, et al. (2001) Somatic activation of the K-ras oncogene causes early onset lung cancer in mice. Nature 410: 1111-1116.
21. Chun MG, Hanahan D (2010) Genetic deletion of the desmosomal component desmoplakin promotes tumor microinvasion in a mouse model of pancreatic neuroendocrine carcinogenesis. PLoS Genet 6.
22. Vijayaraj P, Kroger C, Reuter U, Windoffer R, Leube RE, et al. (2009) Keratins regulate protein biosynthesis through localization of GLUT1 and-3 upstream of AMP kinase and Raptor. J Cell Biol 187: 175-184.
23. Jensen SM, Gazdar AF, Cuttitta F, Russell EK, Linnoila RI, (1990) A comparison of synaptophysin, chromogranin, and L-dopa decarboxylase as markers for neuroendocrine differentiation in lung cancer cell lines. Cancer Res 50: 6068-6074.
24. Kashiwagi K, Ishii J, Sakaeda M, Arimasu Y, Shimoyamada H, et al. (2012) Differences of molecular expression mechanisms among neural cell adhesion molecule 1, synaptophysin, and chromogranin A in lung cancer cells. Pathol Int 62: 232-245.
25. Xiao D, He J, (2010) Epithelial mesenchymal transition and lung cancer. J Thorac Dis 2: 154-159.
26. Jiang YG, Luo Y, He DL, Li X, Zhang LL, et al. (2007) Role of Wnt/beta-catenin signaling pathway in epithelial-mesenchymal transition of human prostate cancer induced by hypoxia-inducible factor-1alpha. Int J Urol 14: 1034-1039.
27. Okayama H, Saito M, Oue N, Weiss JM, Stauffer J, et al. (2012) NOS2 enhances KRAS-induced lung carcinogenesis, inflammation and microRNA-21 expression. Int J Cancer.
28. Lyons SK, Meuwissen R, Krimpenfort P, Berns A, (2003) The generation of a conditional reporter that enables bioluminescence imaging of Cre/loxP-dependent tumorigenesis in mice. Cancer Res 63: 7042-7046.
29. Karantza V, (2011) Keratins in health and cancer: more than mere epithelial cell markers. Oncogene 30: 127-138.
30. Reichelt J, Furstenberger G, Magin TM, (2004) Loss of keratin 10 leads to mitogen-activated protein kinase (MAPK) activation, increased keratinocyte turnover, and decreased tumor formation in mice. J Invest Dermatol 123: 973-981.
31. Chu YW, Seftor EA, Romer LH, Hendrix MJ, (1996) Experimental coexpression of vimentin and keratin intermediate filaments in human melanoma cells augments motility. Am J Pathol 148: 63-69.