Validation of reference genes for expression analysis by quantitative real-time PCR in Leptinotarsa decemlineata (Say)

BMC Research Notes

Volumn 6, Issue 1, 2013, Pages

  Shi, Xiao Qin ^a   Guo, Wen Chao ^b   Wan, Pin Jun ^a   Zhou, Li Tao ^a   Ren, Xiang Liang ^a   Ahmat, Tursun ^b   Fu, Kai Yun ^a   Li, Guo Qing ^a  

^a NANJING AGRICULTURAL UNIVERSITY   (China)

^b INSTITUTE OF MICROBIOLOGY   (China)

Author keywords

L. decemlineata; Normalization; Quantitative real time PCR; Reference gene

Indexed keywords

HEXAPODA; LEPTINOTARSA DECEMLINEATA; SOLANUM TUBEROSUM;

INSECTICIDE; PRIMER DNA;

ANIMAL; ARTICLE; BEETLE; GENE EXPRESSION PROFILING; GENETICS; METHODOLOGY; NUCLEOTIDE SEQUENCE; REAL TIME POLYMERASE CHAIN REACTION; VALIDATION STUDY;

ANIMALS; BASE SEQUENCE; BEETLES; DNA PRIMERS; GENE EXPRESSION PROFILING; INSECTICIDES; REAL-TIME POLYMERASE CHAIN REACTION;

MLCS; MLOWN;

EID: 84874803093     PISSN: None     EISSN: 17560500     Source Type: Journal    
DOI: 10.1186/1756-0500-6-93     Document Type: Article

References (44)

1. Chlorantraniliprole susceptibility in Leptinotarsa decemlineata in the north Xinjiang Uygur autonomous region in China. Jiang W-H, Lu W-P, Guo W-C, Xia Z-H, Fu W-J, Li G-Q, J Econ Entomol 2012 105 2 549 554 10.1603/EC11194 22606826
2. Susceptibilities of Leptinotarsa decemlineata (Say) in the north xinjiang Uygur autonomous region in China to two biopesticides and three conventional insecticides. Lu W-P, Shi X-Q, Guo W-C, Jiang W-H, Xia Z-H, Fu W-J, Li G-Q, J Agr Urban Entomol 2011 27 61 73
3. Efficacy of endosulfan and fipronil and joint toxic action of endosulfan mixtures against Leptinotarsa decemlineata (Say). Shi X-Q, Xiong M-H, Jiang W-H, Wang Z-T, Guo W-C, Xia Z-H, Fu W-J, Li G-Q, J Pest Sci 2012 85 519 526
4. Target site insensitivity mutations in the AChE and LdVssc1 confer resistance 3 to pyrethroids and carbamates in Leptinotarsa decemlineata in northern 4 Xinjiang Uygur autonomous region. Jiang W-H, Guo W-C, Lu W-P, Shi X-Q, Xiong M-H, Li G-Q, Pestic Biochem Phys 2011 100 74 81 10.1016/j.pestbp. 2011.02.008
5. Insecticide resistance status of Colorado potato beetle (Coleoptera: Chrysomelidae) adults in northern Xinjiang Uygur autonomous region. Jiang W-H, Wang Z-T, Xiong M-H, Lu W-P, Liu P, Guo W-C, Li G-Q, J Econ Entomol 2010 103 4 1365 1371 10.1603/EC10031 20857749
6. Colorado potato beetle management on potatoes: Current challenges and future prospects. Alyokhin A, Fruit, Vegetable and Cereal Science and Biotechnology 2009 3 10 19
7. Colorado potato beetle resistance to insecticides. Alyokhin A, Baker M, Mota-Sanchez D, Dively G, Grafius E, Am J Potato Res 2008 85 395 413 10.1007/s12230-008-9052-0
8. Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Vandesompele J, De Preter K, Pattyn F, Poppe B, Van Roy N, De Paepe A, Speleman F, Gen Biol 2002 3 ESEARCH0034
9. Normalization of real-time quantitative reverse transcription-PCR data:a model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data sets. Andersen CL, Jensen JL, Orntoft TF, Cancer Res 2004 64 5245 5250 10.1158/0008-5472.CAN-04- 0496 15289330
10. A technique whose time has come. Walker NJ, Science 2002 296 557 559 10.1126/science.296.5567.557 11964485
11. Validation of reference genes for quantitative expression analysis by real-time RT-PCR in four lepidopteran insects. Teng X, Zhang Z, He G, Yang L, Li F, J Insect Sci 2012 12 60 22938136
12. Validation of reference genes for gene expression studies in the emerald ash borer (Agrilus planipennis). Rajarapu SP, Mamidala P, Mittapalli O, Insect Sci 2012 19 1 41 46 10.1111/j.1744-7917.2011.01447.x
13. Validation of reference genes for expression analysis in the salivary gland and the intestine of Rhodnius prolixus (Hemiptera, Reduviidae) under different experimental conditions by quantitative real-time PCR. Paim RM, Pereira MH, Di Ponzio R, Rodrigues JO, Guarneri AA, Gontijo NF, Araújo RN, BMC Res Notes 2012 5 1 128 10.1186/1756-0500-5-128 22395020
14. Validation of reference genes for gene expression studies in Aphis glycines (Hemiptera: Aphididae). Bansal R, Mamidala P, Mian MAR, Mittapalli O, Michel AP, J Econ Entomol 2012 105 4 1432 1438 10.1603/EC12095 22928326
15. Evaluation of potential reference genes for reverse transcription-qPCR studies of physiological responses in Drosophila melanogaster. Ponton F, Chapuis MP, Pernice M, Sword GA, Simpson SJ, J Insect Physiol 2011 57 6 840 850 10.1016/j.jinsphys.2011.03.014 21435341
16. Reference gene selection for gene expression studies using RT-qPCR in virus-infected planthoppers. Maroniche G, Sagadín M, Mongelli V, Truol G, del Vas M, Virol J 2011 8 1 308 10.1186/1743-422X-8-308 21679431
17. Identification and validation of reference genes for quantitative real-time polymerase chain reaction in Cimex lectularius. Mamidala P, Rajarapu SP, Jones SC, Mittapalli O, J Med Entomol 2011 48 4 947 951 10.1603/ME10262 21845960
18. Assessment and validation of a suite of reverse transcription- quantitative PCR reference genes for analyses of density-dependent behavioural plasticity in the Australian plague locust. Chapuis MP, Tohidi-Esfahani D, Dodgson T, Blondin L, Ponton F, Cullen D, Simpson SJ, Sword GA, BMC Mol Biol 2011 12 1 7 10.1186/1471-2199-12-7 21324174
19. Evaluation of endogenous references for gene expression profiling in different tissues of the oriental fruit fly Bactrocera dorsalis (Diptera: Tephritidae). Shen GM, Jiang HB, Wang XN, Wang JJ, BMC Mol Biol 2010 11 1 76 10.1186/1471-2199-11-76 20923571
20. Evaluation of quantitative PCR reference genes for gene expression studies in Tribolium castaneum after fungal challenge. Lord JC, Hartzer K, Toutges M, Oppert B, J Microbiol Meth 2010 80 2 219 221 10.1016/j.mimet.2009.12. 007
21. Validation of endogenous reference genes for insecticide-induced and developmental expression profiling of Liposcelis bostsrychophila (Psocoptera: Liposcelididae). Jiang H, Liu Y, Tang P, Zhou A, Wang J, Mol Biol Rep 2010 37 1019 1029 10.1007/s11033-009-9803-0 19757170
22. Selection of reference genes for real-time polymerase chain reaction analysis in tissues from Bombus terrestris and Bombus lucorum of different ages. Horáková D, Matoušková P, Kindl J, Valterová I, Pichová I, Anal Biochem 2010 397 1 118 120 10.1016/j.ab.2009.09.019 19751695
23. Identification and validation of housekeeping genes in brains of the desert locust Schistocerca gregaria under different developmental conditions. Van Hiel MB, Van Wielendaele P, Temmerman L, Van Soest S, Vuerinckx K, Huybrechts R, Broeck JV, Simonet G, BMC Mol Biol 2009 10 1 56 10.1186/1471-2199-10-56 19508726
24. Reference genes for QRT-PCR tested under various stress conditions in Folsomia candida and Orchesella cincta (Insecta, Collembola). de Boer M, de Boer T, Marien J, Timmermans M, Nota B, van Straalen N, Ellers J, Roelofs D, BMC Mol Biol 2009 10 54 10.1186/1471-2199-10-54 19486513
25. Reference genes identified in the silkworm Bombyx mori during metamorphism based on oligonucleotide microarray and confirmed by qRT-PCR. Wang GH, Xia QY, Cheng DJ, Duan J, Zhao P, Chen J, Zhu L, Insect Sci 2008 15 405 413 10.1111/j.1744-7917.2008.00227.x
26. Reference gene selection for insect expression studies using quantitative real-time PCR: the head of the honeybee, Apis mellifera, after a bacterial challenge. Scharlaken B, de Graaf DC, Goossens K, Brunain M, Peelman LJ, Jacobs FJ, J Insect Sci 2008 8 33
27. Validation of reference genes for gene expression studies in the honey bee, Apis mellifera, by quantitative real-time RT-PCR. Lourenço AP, Mackert A, Cristino AS, Simões ZLP, Apidologie 2008 39 3 372 385 10.1051/apido:2008015
28. Validation of internal control for gene expression study in soybean by quantitative real-time PCR. Jian B, Liu B, Bi Y, Hou W, Wu C, Han T, BMC Mol Biol 2008 9 59 10.1186/1471-2199-9-59 18573215
29. Ingested RNA interference for managing the populations of the Colorado potato beetle, Leptinotarsa decemlineata. Zhu F, Xu J, Palli R, Ferguson J, Palli SR, Pest Manag Sci 2011 67 175 182 10.1002/ps.2048 21061270
30. Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: BestKeeper-Excel based tool using pair-wise correlations. Pfaffl MW, Tichopad A, Prgomet C, Neuvians TP, Biotechnol Lett 2004 26 509 515 15127793
31. Quantification of mRNA using real-time reverse transcription PCR (RT-PCR): trends and problems. Bustin SA, J Mol Endocrinol 2002 29 23 39 10.1677/jme.0.0290023 12200227
32. Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays. Bustin SA, J Mol Endocrinol 2000 25 169 193 10.1677/jme.0.0250169 11013345
33. Selection of housekeeping genes for gene expression studies in human reticulocytes using real-time PCR. Silver N, Best S, Jiang J, Thein SL, BMC Mol Biol 2006 7 33 10.1186/1471-2199-7-33 17026756
34. Validating internal controls for quantitative plant gene expression studies. Brunner AM, Yakovlev IA, Strauss SH, BMC Plant Biol 2004 4 14 10.1186/1471-2229-4-14 15317655
35. Validation of housekeeping genes as internal control for studying gene expression in rice by quantitative real-time PCR. Jain M, Nijhawan A, Tyagi AK, Khurana JP, Biochem Bioph Res Co 2006 345 646 651 10.1016/j.bbrc.2006.04.140
36. Selection of internal control genes for quantitative real-time RT-PCR studies during tomato development process. Exposito-Rodriguez M, Borges AA, Borges-Perez A, Perez JA, BMC Plant Biol 2008 8 131 10.1186/1471-2229-8-131 19102748
37. The lack of a systematic validation of reference genes: a serious pitfall undervalued in reverse transcription-polymerase chain reaction (RT-PCR) analysis in plants. Gutierrez L, Mauriat M, Guenin S, Pelloux J, Lefebvre JF, Louvet R, Rusterucci C, Moritz T, Guerineau F, Bellini C, Plant Biotechnol J 2008 6 609 618 10.1111/j.1467-7652.2008.00346.x 18433420
38. Bioinformatic analysis of the CLE signaling peptide family. Oelkers K, Goffard N, Weiller GF, Gresshoff PM, Mathesius U, Frickey T, BMC Plant Biol 2008 8 1 10.1186/1471-2229-8-1 18171480
39. Molecular aspects of the cellular activities of ADP-ribosylation factors. Randazzo PA, Nie Z, Miura K, Hsu VW, Sci STKE 2000 http://www.stke.org/cgi/ content/full/OC-sigtrans;2000/59/re1
40. Identification and validation of reference genes for quantitative RT-PCR normalization in wheat. Paolacci AR, Tanzarella OA, Porceddu E, Ciaffi M, BMC Mol Biol 2009 10 1 11 10.1186/1471-2199-10-11 19232096
41. Identification of suitable reference genes for normalization of qPCR data in comparative transcriptomics analyses in the Triticeae. Giménez MJ, Pistón F, Atienza SG, Planta 2011 233 1 163 173 10.1007/s00425-010-1290-y 20960006
42. Reference gene selection for real-time quantitative polymerase chain reaction normalization in Swingle citrumelo under drought stress. Carvalho K, de Campos M, Pereira L, Vieira L, Anal Biochem 2010 402 2 197 199 10.1016/j.ab.2010.03.038 20363209
43. Validation of reference genes for transcription profiling in the salmon louse Lepeophtheirus salmonis, by quantitative real-time PCR. Frost P, Nilsen F, Vet Parasitol 2003 118 169 174 10.1016/j.vetpar.2003.09.020 14651887
44. Beta-actin-an unsuitable internal control for RT-PCR. Selvey S, Thompson EW, Matthaei K, LR A, Irving MG, Griffiths LR, Mol Cell Probe 2001 15 307 311 10.1006/mcpr.2001.0376