Faster protein splicing with the nostoc punctiforme DnaE intein using non-native extein residues

Journal of Biological Chemistry

Volumn 288, Issue 9, 2013, Pages 6202-6211

  Cheriyan, Manoj ^a   Pedamallu, Chandra Sekhar ^a,b,c   Tori, Kazuo ^a,d   Perler, Francine ^a  

^a NEW ENGLAND BIOLABS   (United States)

^b DANA FARBER CANCER INSTITUTE   (United States)

^c BROAD INSTITUTE OF MIT AND HARVARD   (United States)

^d KYUSHU UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

AUTOPROCESSING; CATALYTIC MECHANISMS; DIFFERENT ORIGINS; ENZYME FUNCTIONS; EXTEINS; FLANKING SEQUENCE; GENETIC SELECTION; INSERTION SITE; INTEINS; KANAMYCINS; KINETIC ANALYSIS; NATURALLY OCCURRING; NON-NATIVE; NOSTOC PUNCTIFORME; PEPTIDE BONDS; PROTEIN-SPLICING; SEQUENCE SPACE; SUBSTRATE SPECIFICITY;

AMINO ACIDS; PEPTIDES; SITE SELECTION;

BACTERIA;

AMINO ACID; DNAE INTEIN; INTEIN; KANAMYCIN; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ANTIBIOTIC RESISTANCE; ARTICLE; CONTROLLED STUDY; KINETICS; NONHUMAN; NOSTOC PUNCTIFORME; NUCLEOTIDE SEQUENCE; PLASMID; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PROCESSING; WESTERN BLOTTING;

ANTI-BACTERIAL AGENTS; BACTERIAL PROTEINS; DNA POLYMERASE III; DRUG RESISTANCE, FUNGAL; INTEINS; KANAMYCIN; KINETICS; NOSTOC; PROTEIN SPLICING;

EID: 84874770515     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M112.433094     Document Type: Article

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