Refined preparation and use of anti-diglycine remnant (k-ε-gg) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments

Molecular and Cellular Proteomics

Volumn 12, Issue 3, 2013, Pages 825-831

  Udeshi, Namrata D ^a   Svinkina, Tanya ^a   Mertins, Philipp ^a   Kuhn, Eric ^a   Mani, D R ^a   Qiao, Jana W ^a   Carr, Steven A ^a  

^a BROAD INSTITUTE OF MIT AND HARVARD   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DI GLYCINE REMNANT ANTIBODY; DIGLYCINE; PROTEIN ANTIBODY; UNCLASSIFIED DRUG;

ANTIBODY SPECIFICITY; ARTICLE; DOWN REGULATION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; INHIBITION KINETICS; LEUKEMIA CELL LINE; PH; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN CROSS LINKING; PROTEOMICS; QUANTITATIVE ANALYSIS; REVERSED PHASE LIQUID CHROMATOGRAPHY; UBIQUITINATION;

AMINO ACIDS; ANTIBODIES; BINDING SITES; CHROMATOGRAPHY, LIQUID; CROSS-LINKING REAGENTS; CYSTEINE PROTEINASE INHIBITORS; GLYCYLGLYCINE; HUMANS; ISOTOPE LABELING; JURKAT CELLS; LEUPEPTINS; PROTEASOME ENDOPEPTIDASE COMPLEX; PROTEOME; PROTEOMICS; REPRODUCIBILITY OF RESULTS; TANDEM MASS SPECTROMETRY; UBIQUITINATED PROTEINS; UBIQUITINATION;

EID: 84874619400     PISSN: 15359476     EISSN: 15359484     Source Type: Journal    
DOI: 10.1074/mcp.O112.027094     Document Type: Article

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