Subcellular control of Rac-GTPase signalling by magnetogenetic manipulation inside living cells

Nature Nanotechnology

Volumn 8, Issue 3, 2013, Pages 193-198

  Etoc, F ^a,d   Lisse, D ^b   Bellaiche, Y ^c   Piehler, J ^b   Coppey, M ^a,d   Dahan, M ^a  

^a LABORATOIRE KASTLER BROSSEL   (France)

^b UNIVERSITY OF OSNABRÜCK   (Germany)

^c INSTITUT CURIE   (France)

^d INSTITUT CURIE   (France)

Author keywords

[No Author keywords available]

Indexed keywords

CELLS; COMPLEX NETWORKS; CYTOLOGY; MOLECULAR BIOLOGY; MORPHOLOGY; NANOMAGNETICS; NANOPARTICLES; PROTEINS; SELF ASSEMBLY; SIGNAL TRANSDUCTION;

ACTIN CYTOSKELETON; COORDINATED ACTIVITY; MAGNETIC NANO-PARTICLES; MORPHOLOGICAL CHANGES; SIGNAL TRANSDUCTION PATHWAYS; SIGNALLING COMPLEX; SIGNALLING PATHWAYS; SPATIOTEMPORAL PATTERNS;

CELL SIGNALING;

MAGNETIC NANOPARTICLE; NEURAL WISKOTT ALDRICH SYNDROME PROTEIN; RHO GUANINE NUCLEOTIDE BINDING PROTEIN;

ACTIN FILAMENT; ARTICLE; CELL STRUCTURE; CYTOSOL; GENETIC MANIPULATION; INTRACELLULAR SIGNALING; MAGNETISM; MAGNETOGENETIC MANIPULATION; MICROENVIRONMENT; MOLECULAR DYNAMICS; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN FUNCTION; PROTEIN INTERACTION; SPATIOTEMPORAL ANALYSIS;

EID: 84874605799     PISSN: 17483387     EISSN: 17483395     Source Type: Journal    
DOI: 10.1038/nnano.2013.23     Document Type: Article

References (32)

1. Scott, J. D. & Pawson, T. Cell signalling in space and time: where proteins come together and when they're apart. Science 326, 1220-1224 (2009).
2. Dehmelt, L. & Bastiaens, P. I. Spatial organization of intracellular communication: insights from imaging. Nature Rev. Mol. Cell Biol. 11, 440-452 (2010).
3. Machacek, M. et al. Coordination of Rho GTPase activities during cell protrusion. Nature 461, 99-103 (2009).
4. Pertz, O. Spatio-temporal Rho GTPase signalling-where are we now J. Cell Sci. 123, 1841-1850 (2010).
5. Vartak, N. & Bastiaens, P. Spatial cycles in G-protein crowd control. EMBO J. 29, 2689-2699 (2010).
6. Mayer, G. & Heckel, A. Biologically active molecules with a 'light switch'. Angew. Chem. Int. Ed. 45, 4900-4921 (2006).
7. Umeda, N., Ueno, T., Pohlmeyer, C., Nagano, T. & Inoue, T. A photocleavable rapamycin conjugate for spatiotemporal control of small GTPase activity. J. Am. Chem. Soc. 133, 12-14 (2011).
8. Wu, Y. I. et al. A genetically encoded photoactivatable Rac controls the motility of living cells. Nature 461, 104-108 (2009).
9. Levskaya, A., Weiner, O. D., Lim, W. A. & Voigt, C. A. Spatiotemporal control of cell signalling using a light-switchable protein interaction. Nature 461, 997-1001 (2009).
10. Jun, Y. W., Seo, J. W. & Cheon, J. Nanoscaling laws of magnetic nanoparticles and their applicabilities in biomedical sciences. Acc. Chem. Res. 41, 179-189 (2008).
11. Desprat, N., Supatto, W., Pouille, P. A., Beaurepaire, E. & Farge, E. Tissue deformation modulates twist expression to determine anterior midgut differentiation in Drosophila embryos. Dev. Cell 15, 470-477 (2008).
12. Weber, G. F., Bjerke, M. A. & DeSimone, D.W. A mechanoresponsive cadherin-keratin complex directs polarized protrusive behavior and collective cell migration. Dev. Cell 22, 104-115 (2012).
13. Mannix, R. J. et al. Nanomagnetic actuation of receptor-mediated signal transduction. Nature Nanotech. 3, 36-40 (2008).
14. Cho, M. H. et al. A magnetic switch for the control of cell death signalling in in vitro and in vivo systems. Nature Mater. 11, 1038-1043 (2012).
15. Steketee, M. B. et al. Nanoparticle-mediated signalling endosome localization regulates growth cone motility and neurite growth. Proc. Natl Acad. Sci. USA 108, 19042-19047 (2011).
16. Huang, H., Delikanli, S., Zeng, H., Ferkey, D. M. & Pralle, A. Remote control of ion channels and neurons through magnetic-field heating of nanoparticles. Nature Nanotech. 5, 602-606 (2010).
17. De Vries, A. H., Krenn, B. E., van Driel, R., Subramaniam, V. & Kanger, J. S. Direct observation of nanomechanical properties of chromatin in living cells. Nano Lett. 7, 1424-1427 (2007).
18. Bausch, A. R., Moller, W. & Sackmann, E. Measurement of local viscoelasticity and forces in living cells by magnetic tweezers. Biophys. J. 76, 573-579 (1999).
19. Hendricks, A. G., Holzbaur, E. L. & Goldman, Y. E. Force measurements on cargoes in living cells reveal collective dynamics of microtubule motors. Proc. Natl Acad. Sci. USA 109, 18447-18452 (2012).
20. Wirtz, D. Particle-tracking microrheology of living cells: principles and applications. Annu. Rev. Biophys. 38, 301-326 (2009).
21. Los, G. V. et al. HaloTag: a novel protein labeling technology for cell imaging and protein analysis. ACS Chem. Biol. 3, 373-382 (2008).
22. Lisse, D., Wilkens, V., You, C., Busch, K. & Piehler, J. Selective targeting of fluorescent nanoparticles to proteins inside live cells. Angew. Chem. Int. Ed. 50, 9352-9355 (2011).
23. Etienne-Manneville, S. & Hall, A. Rho GTPases in cell biology. Nature 420, 629-635 (2002).
24. Rohatgi, R. et al. The interaction between N-WASP and the Arp2/3 complex links Cdc42-dependent signals to actin assembly. Cell 97, 221-231 (1999).
25. Rudolph, M. G. et al. Thermodynamics of Ras/effector and Cdc42/effector interactions probed by isothermal titration calorimetry. J. Biol. Chem. 276, 23914-23921 (2001).
26. Van der Gucht, J., Paluch, E., Plastino, J. & Sykes, C. Stress release drives symmetry breaking for actin-based movement. Proc. Natl Acad. Sci. USA 102, 7847-7852 (2005).
27. Michiels, F. et al. Regulated membrane localization of Tiam1, mediated by the NH2-terminal pleckstrin homology domain, is required for Rac-dependent membrane ruffling and C-Jun NH2-terminal kinase activation. J. Cell Biol. 137, 387-398 (1997).
28. Aoki, K. & Matsuda, M. Visualization of small GTPase activity with fluorescence resonance energy transfer-based biosensors. Nature Protoc. 4, 1623-1631 (2009).
29. Riedl, J. et al. Lifeact: a versatile marker to visualize F-actin. Nature Methods 5, 605-607 (2008).
30. Hahne, P., Sechi, A., Benesch, S. & Small, J. V. Scar/WAVE is localised at the tips of protruding lamellipodia in living cells. FEBS Lett. 492, 215-220 (2001).
31. Tseng, P., Judy, J. W. & Di Carlo, D. Magnetic nanoparticle-mediated massively parallel mechanical modulation of single-cell behavior. Nature Methods 9, 1113-1119 (2012).
32. Serge, A., Bertaux, N., Rigneault, H. & Marguet, D. Dynamic multiple-target tracing to probe spatiotemporal cartography of cell membranes. Nature Methods 5, 687-694 (2008).