Deglycosylation-dependent fluorescent proteins provide unique tools for the study of ER-associated degradation

Proceedings of the National Academy of Sciences of the United States of America

Volumn 110, Issue 9, 2013, Pages 3393-3398

  Grotzke, Jeff E ^a   Lu, Qiao ^a   Cresswell, Peter ^a  

^a YALE UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GLUCAN SYNTHASE; HYDROXYMETHYLGLUTARYL COENZYME A REDUCTASE; HYDROXYMETHYLGLUTARYL COENZYME A REDUCTASE 1; PEPTIDES AND PROTEINS; UNCLASSIFIED DRUG; VENUS FLUORESCENT PROTEIN;

ARTICLE; CYTOSOL; ENDOPLASMIC RETICULUM ASSOCIATED DEGRADATION; GLYCOSYLATION AND DEGLYCOSYLATION; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN TRANSPORT;

BACTERIAL PROTEINS; ENDOPLASMIC RETICULUM-ASSOCIATED DEGRADATION; FLUORESCENCE; GENOME, HUMAN; GLYCOSYLATION; HEK293 CELLS; HUMANS; LUMINESCENT PROTEINS; MUTATION; RECOMBINANT FUSION PROTEINS; RNA, SMALL INTERFERING; UBIQUITIN-PROTEIN LIGASES;

EID: 84874506918     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.1300328110     Document Type: Article

References (38)

1. Kanapin A, et al.; RIKEN GER Group; GSL Members (2003) Mouse proteome analysis. Genome Res 13(6B): 1335-1344.
2. Smith MH, Ploegh HL, Weissman JS (2011) Road to ruin: Targeting proteins for degradation in the endoplasmic reticulum. Science 334(6059): 1086-1090.
3. Vembar SS, Brodsky JL (2008) One step at a time: Endoplasmic reticulum-associated degradation. Nat Rev Mol Cell Biol 9(12): 944-957.
4. Chakrabarti A, Chen AW, Varner JD (2011) A review of themammalian unfolded protein response. Biotechnol Bioeng 108(12): 2777-2793.
5. Brodsky JL, McCracken AA (1999) ER protein quality control and proteasome-mediated protein degradation. Semin Cell Dev Biol 10(5): 507-513.
6. Schnell S (2009) A model of the unfolded protein response: Pancreatic beta-cell as a case study. Cell Physiol Biochem 23(4-6): 233-244.
7. Stefani M (2004) Protein misfolding and aggregation: New examples in medicine and biology of the dark side of the protein world. Biochim Biophys Acta 1739(1): 5-25.
8. Christianson JC, Shaler TA, Tyler RE, Kopito RR (2008) OS-9 and GRP94 deliver mutant alpha1-antitrypsin to the Hrd1-SEL1L ubiquitin ligase complex for ERAD. Nat Cell Biol 10(3): 272-282.
9. Hosokawa N, et al. (2008) Human XTP3-B forms an endoplasmic reticulum quality control scaffold with the HRD1-SEL1L ubiquitin ligase complex and BiP. J Biol Chem 283(30): 20914-20924.
10. Lilley BN, Ploegh HL (2004) A membrane protein required for dislocation of misfolded proteins from the ER. Nature 429(6994): 834-840.
11. Ye Y, Shibata Y, Yun C, Ron D, Rapoport TA (2004) A membrane protein complex mediates retro-translocation fromthe ER lumen into the cytosol. Nature 429(6994): 841-847.
12. Kostova Z, Tsai YC, Weissman AM (2007) Ubiquitin ligases, critical mediators of endoplasmic reticulum-associated degradation. Semin Cell Dev Biol 18(6): 770-779.
13. Mehnert M, Sommer T, Jarosch E (2010) ERAD ubiquitin ligases: Multifunctional tools for protein quality control and waste disposal in the endoplasmic reticulum. Bioessays 32(10): 905-913.
14. Carvalho P, Stanley AM, Rapoport TA (2010) Retrotranslocation of a misfolded luminal ER protein by the ubiquitin-ligase Hrd1p. Cell 143(4): 579-591.
15. Braun S, Matuschewski K, Rape M, Thoms S, Jentsch S (2002) Role of the ubiquitinselective CDC48(UFD1/NPL4) chaperone (segregase) in ERAD of OLE1 and other substrates. EMBO J 21(4): 615-621.
16. Jarosch E, et al. (2002) Protein dislocation from the ER requires polyubiquitination and the AAA-ATPase Cdc48. Nat Cell Biol 4(2): 134-139.
17. Rabinovich E, Kerem A, Fröhlich KU, Diamant N, Bar-Nun S (2002) AAA-ATPase p97/Cdc48p, a cytosolic chaperone required for endoplasmic reticulum-associated protein degradation. Mol Cell Biol 22(2): 626-634.
18. Ye Y, Meyer HH, Rapoport TA (2001) The AAA ATPase Cdc48/p97 and its partners transport proteins from the ER into the cytosol. Nature 414(6864): 652-656.
19. Michnick SW, Ear PH, Landry C, Malleshaiah MK, Messier V (2010) A toolkit of proteinfragment complementation assays for studying and dissecting large-scale and dynamic protein-protein interactions in living cells. Methods Enzymol 470: 335-368.
20. Hirsch C, Blom D, Ploegh HL (2003) A role for N-glycanase in the cytosolic turnover of glycoproteins. EMBO J 22(5): 1036-1046.
21. Suzuki T, Seko A, Kitajima K, Inoue Y, Inoue S (1993) Identification of peptide:Nglycanase activity in mammalian-derived cultured cells. Biochem Biophys Res Commun 194(3): 1124-1130.
22. Takahashi N (1977) Demonstration of a new amidase acting on glycopeptides. Biochem Biophys Res Commun 76(4): 1194-1201.
23. Misaghi S, Pacold ME, Blom D, Ploegh HL, Korbel GA (2004) Using a small molecule inhibitor of peptide: N-glycanase to probe its role in glycoprotein turnover. Chem Biol 11(12): 1677-1687.
24. Bernasconi R, Galli C, Calanca V, Nakajima T, Molinari M (2010) Stringent requirement for HRD1, SEL1L, andOS-9/XTP3-B for disposal of ERAD-LS substrates.J CellBiol 188(2): 223-235.
25. Burr ML, et al. (2011) HRD1 and UBE2J1 target misfolded MHC class I heavy chains for endoplasmic reticulum-associated degradation. Proc Natl Acad Sci USA 108(5): 2034-2039.
26. Cattaneo M, et al. (2008) SEL1L and HRD1 are involved in the degradation of unassembled secretory Ig-mu chains. J Cell Physiol 215(3): 794-802.
27. Kikkert M, et al. (2004) Human HRD1 is an E3 ubiquitin ligase involved in degradation of proteins from the endoplasmic reticulum. J Biol Chem 279(5): 3525-3534.
28. Omura T, et al. (2006) A ubiquitin ligase HRD1 promotes the degradation of Pael receptor, a substrate of Parkin. J Neurochem 99(6): 1456-1469.
29. Sifers RN, Brashears-Macatee S, Kidd VJ, Muensch H, Woo SL (1988) A frameshift mutation results in a truncated alpha 1-antitrypsin that is retained within the rough endoplasmic reticulum. J Biol Chem 263(15): 7330-7335.
30. Johnston JA, Ward CL, Kopito RR (1998) Aggresomes: A cellular response to misfolded proteins. J Cell Biol 143(7): 1883-1898.
31. Birmingham A, et al. (2009) Statistical methods for analysis of high-throughput RNA interference screens. Nat Methods 6(8): 569-575.
32. Ye Y, et al. (2005) Recruitment of the p97 ATPase and ubiquitin ligases to the site of retrotranslocation at the endoplasmic reticulum membrane. Proc Natl Acad Sci USA 102(40): 14132-14138.
33. Hosokawa N, Kamiya Y, Kamiya D, Kato K, Nagata K (2009) Human OS-9, a lectin required for glycoprotein endoplasmic reticulum-associated degradation, recognizes mannose-trimmed N-glycans. J Biol Chem 284(25): 17061-17068.
34. Zhong Y, Fang S (2012) Live cell imaging of protein dislocation from the endoplasmic reticulum. J Biol Chem 287(33): 28057-28066.
35. Harada Y, Li H, Li H, LennarzWJ (2009)Oligosaccharyltransferase directly binds to ribosome at a location near the translocon-binding site. Proc Natl Acad Sci USA 106(17): 6945-6949.
36. Aronson DE, Costantini LM, Snapp EL (2011) Superfolder GFP is fluorescent in oxidizing environments when targeted via the Sec translocon. Traffic 12(5): 543-548.
37. Jain RK, Joyce PB, Molinete M, Halban PA, Gorr SU (2001) Oligomerization of green fluorescent protein in the secretory pathway of endocrine cells. Biochem J 360(Pt 3): 645-649.
38. Agmon N (2005) Proton pathways in green fluorescence protein. Biophys J 88(4): 2452-2461.