Homogeneous duplex polymerase chain reaction assay using switchable lanthanide fluorescence probes

Analytical Biochemistry

Volumn 436, Issue 1, 2013, Pages 16-21

  Lehmusvuori, Ari ^a   Tapio, Antti Heikki ^a   Mäki Teeri, Petra ^a   Rantakokko Jalava, Kaisu ^b   Wang, Qi ^a   Takalo, Harri ^a   Soukka, Tero ^a  

^a UNIVERSITY OF TURKU   (Finland)

^b TURKU UNIVERSITY HOSPITAL   (Finland)

Author keywords

Chlamydia trachomatis (Ct); Duplex PCR; Lanthanide chelate; Switchable sensors

Indexed keywords

ANTENNAS; CHELATION; EUROPIUM COMPOUNDS; FLUORESCENCE; LIGANDS; OLIGONUCLEOTIDES; PROBES; RARE EARTH ELEMENTS; TERBIUM COMPOUNDS;

CHLAMYDIA TRACHOMATIS; DUPLEX PCR; INTERNAL AMPLIFICATION; LANTHANIDE CHELATES; LANTHANIDE FLUORESCENCE; SAMPLE PREPARATION METHODS; SIGNAL-TO-BACKGROUND RATIO; SWITCHABLE SENSORS;

POLYMERASE CHAIN REACTION;

EUROPIUM; LANTHANIDE; LIGAND; TERBIUM;

ANTENNA; ARTICLE; CENTRIFUGATION; CHEMICAL STRUCTURE; CHLAMYDIA TRACHOMATIS; CONTROLLED STUDY; DIAGNOSTIC ACCURACY; DNA HYBRIDIZATION; FLUORESCENCE; LIGHT ABSORPTION; PLASMID; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SYNTHESIS; URINALYSIS;

CHLAMYDIA TRACHOMATIS;

CHLAMYDIA INFECTIONS; CHLAMYDIA TRACHOMATIS; DNA, SINGLE-STRANDED; FLUORESCENT DYES; HUMANS; LIGANDS; MOLECULAR STRUCTURE; OLIGONUCLEOTIDE PROBES; REAL-TIME POLYMERASE CHAIN REACTION; SPECTROMETRY, FLUORESCENCE; TERBIUM;

EID: 84874398066     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2013.01.007     Document Type: Article

References (21)

1. J.S. Chamberlain, R.A. Gibbs, J.E. Ranier, P.N. Nguyen, and C.T. Caskey Deletion screening of the Duchenne muscular dystrophy locus via multiplex DNA amplification Nucleic Acids Res. 16 1988 11141 11156
2. M. Rosenstraus, Z. Wang, S.Y. Chang, D. DeBonville, and J.P. Spadoro An internal control for routine diagnostic PCR: design, properties, and effect on clinical performance J. Clin. Microbiol. 36 1998 191 197
3. J. Nurmi, T. Wikman, M. Karp, and T. Lovgren High-performance real-time quantitative RT-PCR using lanthanide probes and a dual-temperature hybridization assay Anal. Chem. 74 2002 3525 3532
4. J. Ragoussis Genotyping technologies for genetic research Annu. Rev. Genomics Hum. Genet. 10 2009 117 133
5. A. Lehmusvuori, U. Karhunen, A.H. Tapio, U. Lamminmaki, and T. Soukka High-performance closed-tube PCR based on switchable luminescence probes Anal. Chim. Acta 731 2012 88 92
6. I. Hemmila, and V. Laitala Progress in lanthanides as luminescent probes J. Fluoresc. 15 2005 529 542
7. J. Mahony, S. Chong, D. Jang, K. Luinstra, M. Faught, D. Dalby, J. Sellors, and M. Chernesky Urine specimens from pregnant and nonpregnant women inhibitory to amplification of Chlamydia trachomatis nucleic acid by PCR, ligase chain reaction, and transcription-mediated amplification: identification of urinary substances associated with inhibition and removal of inhibitory activity J. Clin. Microbiol. 36 1998 3122 3126
8. W. Horrocks Jr., and D. Sudnick Lanthanide ion probes of structure in biology: laser-induced luminescence decay constants provide a direct measure of the number of metal-coordinated water molecules J. Am. Chem. Soc. 101 1979 334 340
9. M. Latva, H. Takalo, V.M. Mukkala, C. Matachescu, J. Rodriguez-Ubis, and J. Kankare Correlation between the lowest triplet state energy level of the ligand and lanthanide(III) luminescence quantum yield J. Lumin. 75 1997 149 169
10. 3+ chelated with various ligands in aqueous solutions: influence of the synergic agent, the surfactant, and the energy level of the ligand triplet Spectrochim. Acta, Part A 59 2003 1829 1840
11. I. Hemmila, V.M. Mukkala, M. Latva, and P. Kiilholma Di- and tetracarboxylate derivatives of pyridines, bipyridines, and terpyridines as luminogenic reagents for time-resolved fluorometric determination of terbium and dysprosium J. Biochem. Biophys. Methods 26 1993 283 290
12. T. Ripa, and P.A. Nilsson A Chlamydia trachomatis strain with a 377-bp deletion in the cryptic plasmid causing false negative nucleic acid amplification tests Sex. Transm. Dis. 34 2007 255 256
13. U. Karhunen, J. Rosenberg, U. Lamminmaki, and T. Soukka Homogeneous detection of avidin based on switchable lanthanide luminescence Anal. Chem. 83 2011 9011 9016
14. U. Karhunen, L. Jaakkola, Q. Wang, U. Lamminmaki, and T. Soukka Luminescence switching by hybridization-directed mixed lanthanide complex formation Anal. Chem. 82 2010 751 754
15. D.D. Root In situ molecular association of dystrophin with actin revealed by sensitized emission immuno-resonance energy transfer Proc. Natl. Acad. Sci. U.S.A. 94 1997 5685 5690
16. M. Angles d'Auriac, U.H. Refseth, M. Espelund, H. Moi, G. Storvold, and S. Jeansson A new automated method for isolation of Chlamydia trachomatis from urine eliminates inhibition and increases robustness for NAAT systems J. Microbiol. Methods 70 2007 416 423
17. T. Engen, M. Anglès d'Auriac, S. Harja, M. Tauvo-Kallio, K. Rantakokko-Jalava, U. Refseth, A new fast automated magnetic bead based nucleic acid sample preparation for STI diagnostics combined with BD ProbeTec ET [poster P917]. < http://www.blackwellpublishing.com/eccmid17/PDFs/clm-1733.pdf > (accessed 18.06.2012).
18. L. Wang, J.R. Blasic Jr., M.J. Holden, and R. Pires Sensitivity comparison of real-time PCR probe designs on a model DNA plasmid Anal. Biochem. 344 2005 257 265
19. D.M. Kong, L. Gu, H.X. Shen, and H.F. Mi A modified molecular beacon combining the properties of TaqMan probe Chem. Commun. 2002 854 855
20. A. Lehmusvuori, E. Juntunen, A.H. Tapio, K. Rantakokko-Jalava, T. Soukka, and T. Lovgren Rapid homogeneous PCR assay for the detection of Chlamydia trachomatis in urine samples J. Microbiol. Methods 83 2010 302 306
21. E. Soini, and T. Lövgren Time-resolved fluorescence of lanthanide probes and applications in biotechnology Crit. Rev. Anal. Chem. 18 1987 105 154