Rapid homogeneous PCR assay for the detection of Chlamydia trachomatis in urine samples

Journal of Microbiological Methods

Volumn 83, Issue 3, 2010, Pages 302-306

  Lehmusvuori, Ari ^a   Juntunen, Etvi ^a   Tapio, Antti Heikki ^a   Rantakokko Jalava, Kaisu ^b   Soukka, Tero ^a   Lövgren, Timo ^a  

^a UNIVERSITY OF TURKU   (Finland)

^b TURKU UNIVERSITY HOSPITAL   (Finland)

Author keywords

Chlamydia trachomatis; Fast homogenous DNA assay; Lanthanide chelate; Non invasive sampling; Time resolved fluorometry

Indexed keywords

LANTHANIDE;

ANALYTIC METHOD; ARTICLE; AUTOMATION; BACTERIUM DETECTION; CENTRIFUGATION; CHLAMYDIA TRACHOMATIS; CONTROLLED STUDY; FLUOROMETRY; HOMOGENOUS POLYMERASE CHAIN REACTION; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROCESS DESIGN; PROCESS TECHNOLOGY; RELIABILITY; SENSITIVITY AND SPECIFICITY; URINALYSIS;

CHLAMYDIA INFECTIONS; CHLAMYDIA TRACHOMATIS; DNA, BACTERIAL; POLYMERASE CHAIN REACTION;

BACTERIA (MICROORGANISMS); CHLAMYDIA TRACHOMATIS;

EID: 78149464484     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mimet.2010.09.018     Document Type: Article

References (28)

1. Anglès d'Auriac M., Refseth U., Espelund M., Moi H., Størvold G., Jeansson S. A new automated method for isolation of Chlamydia trachomatis from urine eliminates inhibition and increases robustness for NAAT systems. J. Microbiol. Methods 2007, 70:416-423.
2. Engen T., Anglès d'Auriac, Harja S., Tauvo-Kallio M., Rantakokko-Jalava K., Refseth U. A new fast automated magnetic bead based nucleic acid sample preparation for STI diagnostics combined with BD ProbeTecTM ET. 17th European Congress of Clinical Microbiology and Infectious Diseases 2007, Accessed at www.blackwellpublishing.com/eccmid17/PDFs/clm_1733.pdf on 20 august 2010.
3. Galvin S., Cohen M. The role of sexually transmitted diseases in HIV transmission. Nat. Rev. Microbiol. 2004, 2:33-42.
4. Gaydos C. Rapid tests for sexually transmitted diseases. Curr. Infect. Dis. Rep. 2006, 8:115-124.
5. Gaydos C., Quinn T., Willis D., Weissfeld A., Hook E., Martin D., Ferrero D., Schachter J. Performance of the APTIMA Combo 2 assay for detection of Chlamydia trachomatis and Neisseria gonorrhoeae in female urine and endocervical swab specimens. J. Clin. Microbiol. 2003, 41:304-309.
6. Gaydos C., Theodore M., Dalesio N., Wood B., Quinn T. Comparison of three nucleic acid amplification tests for detection of Chlamydia trachomatis in urine specimens. J. Clin. Microbiol. 2004, 42:3041-3045.
7. Hadad R., Fredlund H., Unemo M. Evaluation of the new COBAS TaqMan CT test v2.0 and impact on the proportion of new variant Chlamydia trachomatis by the introduction of diagnostics detecting new variant C trachomatis in Orebro county, Sweden. Sex. Transm. Infect. 2009, 85:190-193.
8. Hagren V., von Lode P., Syrjälä A., Soukka T., Lövgren T., Kojola H., Nurmi J. An automated PCR platform with homogeneous time-resolved fluorescence detection and dry chemistry assay kits. Anal. Biochem. 2008, 374:411-416.
9. Hemmilä I., Mukkala V.-M. Time-resolution in fluorometry technologies, labels, and applications in bioanalytical assays. Crit. Rev. Clin. Lab. Sci. 2001, 38:441-519.
10. Honey E., Augood C., Templeton A., Russell I., Paavonen J., Mårdh P., Stary A., Stray-Pedersen B. Cost effectiveness of screening for Chlamydia trachomatis: a review of published studies. Sex. Transm. Infect. 2002, 78:406-412.
11. Hook E.r., Spitters C., Reichart C., Neumann T., Quinn T. Use of cell culture and a rapid diagnostic assay for Chlamydia trachomatis screening. JAMA 1994, 272:867-870.
12. Kiviniemi M., Nurmi J., Turpeinen H., Lövgren T., Ilonen J. A homogeneous high-throughput genotyping method based on competitive hybridization. Clin. Biochem. 2003, 36:633-640.
13. Mabey D., Peeling R., Perkins M. Rapid and simple point of care diagnostics for STIs. Sex. Transm. Infect. 2001, 77:397-398.
14. Marshall R., Chernesky M., Jang D., Hook E., Cartwright C., Howell-Adams B., Ho S., Welk J., Lai-Zhang J., Brashear J., Diedrich B., Otis K., Webb E., Robinson J., Yu H. Characteristics of the m2000 automated sample preparation and multiplex real-time PCR system for detection of Chlamydia trachomatis and Neisseria gonorrhoeae. J. Clin. Microbiol. 2007, 45:747-751.
15. Morrison L., Halder T., Stols L. Solution-phase detection of polynucleotides using interacting fluorescent labels and competitive hybridization. Anal. Biochem. 1989, 183:231-244.
16. Nurmi J., Wikman T., Karp M., Lövgren T. High-performance real-time quantitative RT-PCR using lanthanide probes and a dual-temperature hybridization assay. Anal. Chem. 2002, 74:3525-3532.
17. Nurmi, J., Syrjälä, A., von Lode, P., Hagren, V. Method for the detection of the presence of a target polynucleotide in a sample, Patent application FI20070203, 2007.
18. Palmer L., Falkow S. A common plasmid of Chlamydia trachomatis. Plasmid 1986, 16:52-62.
19. Peeling R. Testing for sexually transmitted infections: a brave new world?. Sex. Transm. Infect. 2006, 82:425-430.
20. Ripa T., Nilsson P. A Chlamydia trachomatis strain with a 377-bp deletion in the cryptic plasmid causing false-negative nucleic acid amplification tests. Sex. Transm. Dis. 2007, 34:255-256.
21. Schachter J., Hook E., Martin D., Willis D., Fine P., Fuller D., Jordan J., Janda W., Chernesky M. Confirming positive results of nucleic acid amplification tests (NAATs) for Chlamydia trachomatis: all NAATs are not created equal. J. Clin. Microbiol. 2005, 43:1372-1373.
22. Schwebke J., Sadler R., Sutton J., Hook E.r. Positive screening tests for gonorrhea and chlamydial infection fail to lead consistently to treatment of patients attending a sexually transmitted disease clinic. Sex. Transm. Dis. 1997, 24:181-184.
23. Unemo M., Rossouw A., James V., Jenkins C. Can the Swedish new variant of Chlamydia trachomatis (nvCT) be detected by UK NEQAS participants from seventeen European countries and five additional countries/regions in 2009?. Euro Surveill. 2009, 14.
24. von Lode P., Rosenberg J., Pettersson K., Takalo H. A europium chelate for quantitative point-of-care immunoassays using direct surface measurement. Anal. Chem. 2003, 75:3193-3201.
25. von Lode P., Syrjälä A., Hagren V., Kojola H., Soukka T., Lövgren T., Nurmi J. Fully automated, homogeneous nucleic acid detection technology based on dry-reagent assay chemistry and time-resolved fluorometry. Clin. Chem. 2007, 53:2014-2017.
26. Walsh A., Rourke F., Laoi B., Crowley B. Evaluation of the Abbott RealTime CT assay with the BD ProbeTec ET assay for the detection of Chlamydia trachomatis in a clinical microbiology laboratory. Diagn. Microbiol. Infect. Dis. 2009, 64:13-19.
27. Whiley D., Sloots T. Comparison of three in-house multiplex PCR assays for the detection of Neisseria gonorrhoeae and Chlamydia trachomatis using real-time and conventional detection methodologies. Pathology 2005, 37:364-370.
28. World Health Organization Global prevalence and incidence of selected curable sexually transmitted infections: overview and estimates 2001, World Health Organization, Geneva, Accessed at www.who.int/hiv/pub/sti/who_hiv_aids_2001.02.pdf on 12 December 2009.