Urine specimens from pregnant and nonpregnant women inhibitory to amplification of Chlamydia trachomatis nucleic acid by PCR, ligase chain reaction, and transcription-mediated amplification: Identification of urinary substances associated with inhibition and removal of inhibitory activity

Journal of Clinical Microbiology

Volumn 36, Issue 11, 1998, Pages 3122-3126

  Mahony, J ^a,b   Chong, S ^a   Jang, D ^a   Luinstra, K ^a   Faught, M ^a   Dalby, D ^b   Sellors, J ^a,b   Chernesky, M ^a,b  

^a MCMASTER UNIVERSITY   (Canada)

^b ST JOSEPH S HOSPITAL   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

CHLOROFORM; CHORIONIC GONADOTROPIN BETA SUBUNIT; HEMOGLOBIN; NITRITE; NUCLEIC ACID; PHENOL;

ADOLESCENT; ADULT; ARTICLE; CHLAMYDIA TRACHOMATIS; CHLAMYDIASIS; CONTROLLED STUDY; FEMALE; GENETIC TRANSCRIPTION; HUMAN; LIGASE CHAIN REACTION; MAJOR CLINICAL STUDY; POLYMERASE CHAIN REACTION; PREGNANCY; PRIORITY JOURNAL; PROSPECTIVE STUDY; REGRESSION ANALYSIS; URINALYSIS;

CHLAMYDIA TRACHOMATIS; RICKETTSIALES;

EID: 0031692077     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/jcm.36.11.3122-3126.1998     Document Type: Article

References (19)

1. Berg, E. S., G. Anestad, H. Moi, G. Storvold, and K. Skaug. 1997. False-negative results of a ligase chain reaction assay to detect Chlamydia trachomatis due to inhibitors in urine. Eur. J. Clin. Microbiol. Infect. Dis. 16:727-731.
2. Chernesky, M. A., D. Jang, H. Lee, J. D. Burczak, H. Hu, J. Sellors, S. J. Tomazic-Allen, and J. B. Mahony. 1994. Diagnosis of Chlamydia trachomatis infections in men and women by testing first-void urine by ligase chain reaction. J. Clin. Microbiol. 32:2682-2685.
3. Chernesky, M. A., D. Jang, J. Sellors, K. Luinstra, S. Chong, S. Castriciano, and J. B. Mahony. 1997. Urinary inhibitors of polymerase chain reaction and ligase chain reaction and testing of multiple specimens may contribute to lower assay sensitivities for diagnosing Chlamydia trachomatis infected women. Mol. Cell. Probes 11:243-249.
4. Chong, S., J. B. Mahony, D. Jang, K. Luinstra, M. Chernesky. 1996. Inhibitors of Chlamydia trachomatis PCR in urine, abstr. D3. In 64th Conjoint Meeting on Infectious Diseases.
5. Crotchfelt, K. A., B. Pare, C. Gaydos, and T. C. Quinn. 1998 Detection of Chlamydia trachomatis by the Gen-Probe AMPLIFIED Chlamvdia trachomatis Assay (AMP-CT) in urine specimens from men and women and endocervical specimens from women. J. Clin. Microbiol. 36:391-394.
6. de Barbeyrac, B., P. Rodriguez, B. Dutilh, P. Le Roux, and C. Bebear. 1995. Detection of Chlamvdia trachomatis by ligase chain reaction and cell culture in urogenital specimens. Genitourin. Med. 71:382-386.
7. Forbes, B. A., and K. E. Hicks. 1996. Substances interfering with direct detection of Mycobacterium tuberculosis in clinical specimens by PCR: effects of bovine serum albumin. J. Clin. Microbiol. 34:2125-2128.
8. Gaydos, C. A., Y. F. Ngeow, H. H. Lee, M. Canavaggio, L. E. Welsh, J. Johanson, and T. C. Quinn. 1995. Urine as a diagnostic specimen for the detection of Chlamydia trachomatis in Malaysia by ligase chain reaction. Sex. Transm. Dis. 23:402-406.
9. Jensen, I. P., P. Thorsen, and B. R. Moller. 1997. Sensitivity of ligase chain reaction assay of urine from pregnant women for Chlamydia trachomatis. Lancet 349:329-330.
10. Lee, H. H., M. A. Chernesky, J. Schachter, J. D. Burczak, W. W. Andrews, S. Muldoon, G. Leckie, and W. E. Stamm. 1995. Diagnosis of Chlamydia trachomatis genitourinary infection in women by ligase chain reaction assay of urine. Lancet 345:213-216.
11. Miettinen, A., P. Vuorinen, T. Varis, and O. Hallstrom. 1995. Comparison of enzyme immunoassay antigen detection, nucleic acid hybridization and PCR assay in the diagnosis of Chlamydia trachomatis infection. Eur. J. Clin. Microbiol. Infect. Dis. 14:546-549.
12. Mouton, J. W., R. Verkooyen, W. I. Meijden, T. H. van Rijsoort-Vos, W. H. F. Goessens, J. A. J. W. Kluytmans, S. D. A. Deelen, A. Luijendijk, and H. A. Verbrugh. 1997. Detection of Chlamydia trachomatis in male and female urine specimens by using the amplified Chlamydia trachomatis test. J. Clin. Microbiol. 35:1369-1372.
13. Rabenau, H., A. Berger, H. W. Doerr, and B. Weber. 1997. Testing for Chlamydia trachomatis in urine. Lancet 349:1024-1025.
14. Rosenstraus, M., Z. Wang, S.-Y. Chang, D. DeBonville, and J. Spadoro. 1998. An internal control for routine diagnostic PCR: design, properties, and effect on clinical performance. J. Clin. Microbiol. 36:191-197.
15. Schepetiuk, S., T. Kok, L. Martin, R. Waddell, and G. Higgins. 1997. Detection of Chlamydia trachomatis in urine samples by nucleic acid tests: comparison with culture and enzyme immunoassay of genital swab specimens. J. Clin. Microbiol. 35:3355-3357.
16. Stary, A., B. Choueiri, and I. Horting-Muller. 1996. Detection of Chlamydia trachomatis in urethral and urine samples from symptomatic and asymptomatic male patients by polymerase chain reaction. Eur. J. Clin. Microbiol. Infect. Dis. 15:465-471.
17. Strasinger, S. K. 1985. Urinalysis and body fluids, p. 205-208. F. A. Davis Co., Philadelphia, Pa.
18. Verkooyen, R. P., A. Luijendijk, W. M. Huisman, W. H. F. Goessens, J. A. J. W. Kluytmans, J. H. van Rijsoort-Vos, and H. A. Verbrugh. 1996. Detection of PCR inhibitors in cervical specimens by using the AMPLICOR Chlamydia trachomatis assay. J. Clin. Microbiol. 34:3072-3074.
19. Wiedbrauk, D. L., J. C. Werner, and A. M. Drevon. 1995. Inhibition of PCR by aqueous and vitreous fluids. J. Clin. Microbiol. 33:2643-2646.