Preliminary evaluation of a procedure for improved detection of Shiga toxin-producing Escherichia coli in fecal specimens

Diagnostic Microbiology and Infectious Disease

Volumn 65, Issue 1, 2009, Pages 21-26

  Hu, Jinxin ^a   Green, Donna ^b   Swoveland, Jennifer ^b   Grant, Michael ^a   Boyle, David S ^b  

^a CENTER FOR DRUG EVALUATION AND RESEARCH   (United States)

^b WASHINGTON STATE DEPARTMENT OF HEALTH   (United States)

Author keywords

Acid Enrichment; Clinical fecal specimens; Enzyme Immunoassay (EIA); Rainbow agar plus tellurite and novobiocin (RTN); Shiga Toxin Producing E. Coli (STEC); Sorbitol MacConkey agar plus cefixime and tellurite (TCSMAC)

Indexed keywords

AGAR; CEFIXIME; NOVOBIOCIN; SHIGA TOXIN; SORBITOL; VEROTOXIN 1; VEROTOXIN 2;

ARTICLE; BACTERIAL GROWTH; BACTERIUM CULTURE; BACTERIUM DETECTION; BACTERIUM IDENTIFICATION; BACTERIUM ISOLATION; CONTROLLED STUDY; CULTURE MEDIUM; ESCHERICHIA COLI; FECES ANALYSIS; IMMUNOASSAY; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; SHIGA TOXIN PRODUCING ESCHERICHIA COLI;

BACTERIOLOGICAL TECHNIQUES; DNA, BACTERIAL; ESCHERICHIA COLI INFECTIONS; ESCHERICHIA COLI PROTEINS; FECES; HUMANS; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SEROTYPING; SHIGA TOXIN 1; SHIGA TOXIN 2; SHIGA-TOXIGENIC ESCHERICHIA COLI;

EID: 68249128659     PISSN: 07328893     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.diagmicrobio.2009.05.012     Document Type: Article

References (17)

1. Anonymous. Importance of culture confirmation of Shiga toxin-producing Escherichia coli infection as illustrated by outbreaks of gastroenteritis-New York and North Carolina, 2005. MMWR 55 (2006) 1042-1045
2. Anonymous. Laboratory-confirmed non-O157 Shiga toxin-producing Escherichia coli-Connecticut, 2000-2005. MMWR 56 (2007) 29-31
3. Bettleheim K.A. Reliability of O157:H7 ID agar (O157 ID-F) for the detection and isolation of verocytotoxigenic strains of Escherichia coli belonging to serogroup O157. J. Appl. Microbiol. 99 (2005) 408-410
4. Brooks J., Sowers E.G., Wells J.G., Greene K.D., Griffin P.M., Hoekstra R.M., and Strockbine N.A. Non-O157 Shiga toxin-producing Escherichia coli infections in the United States, 1983-2002. J. Infect. Dis. 192 (2005) 1422-1429
5. Fey P.D., Wickert R.S., Rupp M.E., Safranek T.J., and Hinrichs S.H. Prevalence of non-O157:H7 Shiga toxin-producing Escherichia coli in diarrheal stool samples from Nebraska. Emerg. Infect. Dis. 6 (2000) 530-533
6. Grant M.A. Improved laboratory enrichment for enterohemorrhagic Escherichia coli by exposure to extremely acidic conditions. Appl. Environ. Microbiol. 70 (2004) 1226-1230
7. Grant M.A. Comparison of a new enrichment procedure for Shiga toxin-producing Escherichia coli with five standard methods. J. Food Protect. 68 (2005) 1593-1599
8. Grant M.A. Comparison of Escherichia coli O157:H7 enrichment in spiked produce samples. J. Food Prot. 71 (2008) 139-145
9. Herold S., Karch H., and Schmidt H. Shiga toxin-encoding bacteriophages-genomes in motion. Int. J. Med. Microbiol. 294 (2004) 115-121
10. Jelacic J.K., Damrow T., Chen G.S., et al. Shiga toxin-producing Escherichia coli in Montana: bacterial genotypes and clinical profiles. J. Infect. Dis. 188 (2003) 719-729
11. Jenkins C., Willshaw G.A., Evans J., et al. Subtyping of virulence genes in verocytotoxin-producing Escherichia coli (VTEC) other than serogroup O157 associated with disease in the United Kingdom. J. Med. Microbiol. 52 (2003) 941-947
12. Kehl K.S., Havens P., Behnke C.E., and Acheson D.W.K. Evaluation of the premier EHEC assay for detection of Shiga toxin-producing Escherichia coli. J. Clin. Microbiol. 35 (1997) 2051-2054
13. Klein E.J., Stapp J.R., Clausen C.R., Boster D.R., Wells J.G., Qin X., Swerdlow D.L., and Tarr P.I. Shiga toxin-producing Escherichia coli in children with diarrhea: a prospective point-of-care study. J. Pediatr. 141 (2002) 172-178
14. Pao S., Patel D., Kalatari A., Tritschler J.P., Wildeus S., and Sayre B.L. Detection of Salmonella strains and Escherichia coli O157:H7 in feces of small ruminants and their isolation with various media. Appl. Environ. Microbiol. 71 (2005) 2158-2161
15. Sharma K., and Dean-Nystrom E.A. Detection of enterohemorrhagic Escherichia coli O157:H7 by using a multiplex real-time PCR assay for genes encoding intimin and Shiga toxins. Vet. Microbiol. 93 (2003) 247-260
16. Stapp J.R., Jelacic S., Yea Y.L., Klein E.J., Fischer M., Clausen C.R., Qin X., Swerdlow D.L., and Tarr P.I. Comparison of Escherichia coli O157:H7 antigen detection in stool and broth cultures to that in sorbitol-MacConkey agar stool cultures. J. Clin. Microbiol. 38 (2000) 3404-3406
17. Teel L.D., Daly J.A., Jerris R.C., Maul D., Svanas G., O'Brien A.D., and Park C.H. Rapid detection of Shiga toxin-producing Escherichia coli by optical immunoassay. J. Clin. Microbiol. 45 (2007) 3377-3380