A novel expression system for intracellular production and purification of recombinant affinity-tagged proteins in Aspergillus niger

Applied Microbiology and Biotechnology

Volumn 86, Issue 2, 2010, Pages 659-670

  Roth, Andreas H F J ^a   Dersch, Petra ^a,b  

^a TECHNICAL UNIVERSITY OF BRAUNSCHWEIG   (Germany)

^b HELMHOLTZ CENTRE FOR INFECTION RESEARCH   (Germany)

Author keywords

Affinity tagged proteins; Aspergillus niger; Expression vectors; Inducible promoter; Protein production; SucA promoter

Indexed keywords

ASPERGILLUS NIGER; EXPRESSION VECTORS; INDUCIBLE PROMOTER; PROTEIN PRODUCTION; TAGGED PROTEINS;

AFFINITY CHROMATOGRAPHY; CHROMATOGRAPHIC ANALYSIS; CLONING; GENE EXPRESSION; PURIFICATION; SUGAR (SUCROSE); TARGETS; VECTORS;

PROTEINS;

BETA FRUCTOFURANOSIDASE; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; HYDROLASE; PLASMID VECTOR; RECOMBINANT PROTEIN; SUCROSE;

CHROMATOGRAPHY; FUNGUS; GENE; PLASMID; PROTEIN;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; ASPERGILLUS NIGER; CARBON SOURCE; CARBOXY TERMINAL SEQUENCE; EXPRESSION VECTOR; FUNGAL GENE; GENE EXPRESSION REGULATION; GENE EXPRESSION SYSTEM; MOLECULAR CLONING; NONHUMAN; PROMOTER REGION; PROTEIN PURIFICATION; PROTEIN SYNTHESIS; THERMOBIFIDA FUSCA; THERMOPHILIC BACTERIUM;

ASPERGILLUS NIGER; BIOTECHNOLOGY; DNA, FUNGAL; GENE EXPRESSION; GENE EXPRESSION REGULATION, FUNGAL; GENETIC VECTORS; MOLECULAR SEQUENCE DATA; PROMOTER REGIONS, GENETIC; RECOMBINANT FUSION PROTEINS; RECOMBINANT PROTEINS; SEQUENCE ANALYSIS, DNA;

ASPERGILLUS NIGER; THERMOBIFIDA FUSCA;

EID: 77249093008     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-009-2252-9     Document Type: Article

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