Co-expression of Erns and E2 genes of classical swine fever virus by replication-defective recombinant adenovirus completely protects pigs against virulent challenge with classical swine fever virus

Research in Veterinary Science

Volumn 94, Issue 2, 2013, Pages 354-360

  Sun, Yongke ^a   Yang, Yuai ^a   Zheng, Huanli ^a   Xi, Dongmei ^a   Lin, Mingxing ^a   Zhang, Xiaomin ^a   Yang, Linfu ^a   Yan, Yulin ^a   Chu, Xiaohui ^a   Bi, Baoliang ^a  

^a YUNNAN AGRICULTURAL UNIVERSITY   (China)

Author keywords

Classical swine fever (CSF); Human adenovirus; Protection; Recombinant; Vaccine

Indexed keywords

GLYCOPROTEIN; NUCLEOCAPSID PROTEIN; STRUCTURAL PROTEIN; VIRUS RNA; VIRUS VACCINE;

ARTICLE; CONTROLLED STUDY; E2 GENE; ERNS GENE; GENE; GENE EXPRESSION; HUMAN ADENOVIRUS 5; INOCULATION; NONHUMAN; PESTIVIRUS; SWINE; VACCINATION; VIRUS REPLICATION; VIRUS STRAIN; VIRUS VIRULENCE;

ADENOVIRIDAE; ANIMALS; CLASSICAL SWINE FEVER; CLASSICAL SWINE FEVER VIRUS; GENE EXPRESSION REGULATION, VIRAL; HEK293 CELLS; HUMANS; SWINE; VIRAL PROTEINS; VIRULENCE; VIRUS REPLICATION;

ADENOVIRIDAE; ANIMALIA; CLASSICAL SWINE FEVER VIRUS; HUMAN ADENOVIRUS; HUMAN ADENOVIRUS TYPE 5; SUIDAE;

EID: 84873723856     PISSN: 00345288     EISSN: 15322661     Source Type: Journal    
DOI: 10.1016/j.rvsc.2012.09.012     Document Type: Article

References (41)

1. Virus Taxonomy: VIIIth Report of the International Committee On Taxonomy of Viruses (ICTV) 2005, Elsevier, Academic Press, Hong Kong. C.M. Fauquet, M.A. Mayo, J. Maniloff, L.A. Ball (Eds.).
2. Moennig V., Floegel-Niesmann G., Greiser-Wilke I. Clinical signs and epidemiology of classical swine fever: a review of new knowledge. Veterinary Journal 2003, 165:11-20.
3. OIE, 2009a. Handistatus II. Available from: .
4. OIE, 2009b. WAHID. Available from: .
5. Dahle J., Liess B. A review on classical swine fever infections in pigs: epizootiology, clinical disease and pathology. Comparative Immunology, Microbiology & Infectious Diseases 1992, 15:203-211.
6. Terpstra C., Woortmeyer R., Barteling S.J. Development and properties of a cell culture produced vaccine for hog cholera based on the Chinese strain. Deutsche Tierarztliche Wochenschrift 1990, 97:77-79.
7. Meeuwissen M.P.M., Horst S.H., Huirne R.B.M., Dijkhuizen A.A. A model to estimate the financial consequences of classical swine fever outbreaks: principles and outcomes. Preventive Veterinary Medicine 1999, 42:249-270.
8. Penrith M.L., Vosloo W., Mather C. Classical swine fever (hog cholera): review of aspects relevant to control. Transboundary and Emerging Diseases 2011, 58:187-196.
9. Meyers G., Thiel H.J. Molecular characterization of pestiviruses. Advances in Virus Research 1996, 47:58-118.
10. Riedel C., Lamp B., Heimann M., Rümenapf T. Characterization of essential domains and plasticity of the classical swine fever virus core protein. Journal of Virology 2010, 84:11523-11531.
11. Lin M., Trottier E., Pasick J., Sabara M. Identification of antigenic regions of the Erns protein for pig antibodies elicited during classical swine fever virus infection. Journal of Biochemistry 2004, 136:795-804.
12. Langedijk J.P., Middel W.G., Meloen R.H., Kramps J.A., de Smit J.A. Enzyme-linked immunosorbent assay using a virus type-specific peptide based on a subdomain of envelope protein Erns for serologic diagnosis of pestivirus infections in swine. Journal of Clinical Microbiology 2001, 39:906-912.
13. Sun Y., Li H.Y., Zhang X.J., Chang T.M., He F., Wang X.P., Liu D.F., Qiu H.J. Comparison of the protective efficacy of recombinant adenoviruses against classical swine fever. Immunology Letters 2011, 135:43-49.
14. Wu C.M., Hsuan S.L., Chen Z.W., Jinn T.R., Huang C., Liao J.W., Chen T.H., Liao C.M., Lee W.C., Wu T.Y., Chien M.S. Expression and immunological studies of classical swine fever virus glycoprotein E2 in the bi-cistronic baculovirus/larvae expression system. Bioscience, Biotechnology and Biochemistry 2010, 74:1343-1349.
15. Hammond J.M., Jansen E.S., Morrissy C.J., Hodgson A.L., Johnson M.A. Protection of pigs against 'in contact' challenge with classical swine fever following oral or subcutaneous vaccination with a recombinant porcine adenovirus. Virus Research 2003, 97:151-157.
16. Reimann I., Depner K., Utke K., Leifer I., Lange E., Beer M. Characterization of a new chimeric marker vaccine candidate with a mutated antigenic E2-epitope. Veterinary Microbiology 2010, 142:45-50.
17. Leifer I., Lange E., Reimann I., Blome S., Juanola S., Duran J.P., Beer M. Modified live marker vaccine candidate CP7_E2alf provides early onset of protection against lethal challenge infection with classical swine fever virus after both intramuscular and oral immunization. Vaccine 2009, 27:6522-6529.
18. Holinka L.G., Fernandez-Sainz I., O'Donnell V., Prarat M.V., Gladue D.P., Lu Z., Risatti G.R., Borca M.V. Development of a live attenuated antigenic marker classical swine fever vaccine. Virology 2009, 384:106-113.
19. Tarradas J., Argilaguet J.M., Rosell R., Nofrarías M., Crisci E., Córdoba L., Pérez-Martín E., Díaz I., Rodríguez F., Domingo M., Montoya M., Ganges L. Interferon-gamma induction correlates with protection by DNA vaccine expressing E2 glycoprotein against classical swine fever virus infection in domestic pigs. Veterinary Microbiology 2010, 142:51-58.
20. Ganges L., Barrera M., Núñez J.I., Blanco I., Frias M.T., Rodríguez F., Sobrino F. A DNA vaccine expressing the E2 protein of classical swine fever virus elicits T cell responses that can prime for rapid antibody production and confer total protection upon viral challenge. Vaccine 2005, 23:3741-3752.
21. Andrew M.E., Morrissy C.J., Lenghaus C., Oke P.G., Sproat K.W., Hodgson A.L., Johnson M.A., Coupar B.E. Protection of pigs against classical swine fever with DNA-delivered gp55. Vaccine 2000, 18:1932-1938.
22. Mast T.C., Kierstead L., Gupta S.B., Nikas A.A., Kallas E.G., Novitsky V., Mbewe B., Pitisuttithum P., Schechter M., Vardas E., Wolfe N.D., Aste-Amezaga M., Casimiro D.R., Coplan P., Straus W.L., Shiver J.W. International epidemiology of human pre-existing adenovirus (Ad) type-5, type-6, type-26 and type-36 neutralizing antibodies: correlates of high Ad5 titers and implications for potential HIV vaccine trials. Vaccine 2010, 28:950-957.
23. Mesonero A., Suarez D.L., Van Santen E., Tang D.C., Toro H. Avian influenza in ovo vaccination with replication defective recombinant adenovirus in chickens: vaccine potency, antibody persistence, and maternal antibody transfer. Avian Diseases 2011, 55:285-292.
24. Fernández E., Toledo J.R., Chiong M., Parra F., Rodríguez E., Montero C., Méndez L., Capucci L., Farnós O. Single dose adenovirus vectored vaccine induces a potent and long-lasting immune response against rabbit hemorrhagic disease virus after parenteral or mucosal administration. Veterinary Immunology and Immunopathology 2011, 142:179-188.
25. Peiperl L., Morgan C., Moodie Z., Li H., Russell N., Graham B.S., Tomaras G.D., De Rosa S.C., McElrath M.J. NIAID HIV Vaccine Trials Network. Safety and immunogenicity of a replication-defective adenovirus type 5 HIV vaccine in Ad5-seronegative persons: a randomized clinical trial (HVTN 054). PLoS ONE 2010, 5:e13579.
26. Sun Y., Liu D.F., Wang Y.F., Liang B.B., Cheng D., Li N., Qi Q.F., Zhu Q.H., Qiu H.J. Generation and efficacy evaluation of a recombinant adenovirus expressing the E2 protein of classical swine fever virus. Research in Veteriany Science 2010, 88:77-82.
27. Tsao Y.S., Condon R., Schaefer E., Lio P., Liu Z. Development and improvement of a serum-free suspension process for the production of recombinant adenoviral vectors using HEK293 cells. Cytotechnology 2001, 37:189-198.
28. Terpstra C., Bloemraad M., Gielkens A.L. The neutralizing peroxidase-linked assay for detection of antibody against swine fever virus. Veterinary Microbiology 1984, 9:113-120.
29. Vlasova A., Grebennikova T., Zaberezhny A., Greiser-Wilke I., Floegel-Niesmann G., Kurinnov V., Aliper T., Nepoklonov E. Molecular epidemiology of classical swine fever in the Russian Federation. Journal of Veterinary Medicine. B, Infectious Diseases and Veterinary Public Health 2003, 50:363-367.
30. Sánchez O., Barrera M., Rodríguez M.P., Frías M.T., Figueroa N.E., Naranjo P., Montesino R., Farnos O., Castell S., Venereo A., Ganges L., Borroto C., Toledo J.R. Classical swine fever virus E2 glycoprotein antigen produced in adenovirally transduced PK-15 cells confers complete protection in pigs upon viral challenge. Vaccine 2008, 26:988-997.
31. Hammond J.M., McCoy R.J., Jansen E.S., Morrissy C.J., Hodgson A.L., Johnson M.A. Vaccination with a single dose of a recombinant porcine adenovirus expressing the classical swine fever virus gp55 (E2) gene protects pigs against classical swine fever. Vaccine 2000, 18:1040-1050.
32. Sun Y., Liu D.F., Wang Y.F., Liang B.B., Cheng D., Li N., Qi Q.F., Zhu Q.H., Qiu H.J. Generation and efficacy evaluation of a recombinant adenovirus expressing the E2 protein of classical swine fever virus. Research in Veterinary Science 2010, 88:77-82.
33. Rümenapf T., Stark R., Meyers G., Thiel H.J. Structural proteins of hog cholera virus expressed by vaccinia virus: further characterization and induction of protective immunity. Journal of Virology 1991, 65:589-597.
34. Hammond J.M., Jansen E.S., Morrissy C.J., Williamson M.M., Hodgson A.L., Johnson M.A. Oral and sub-cutaneous vaccination of commercial pigs with a recombinant porcine adenovirus expressing the classical swine fever virus gp55 gene. Archives of Virology 2001, 146(9):1787-1793.
35. Crawford-Miksza L., Schnurr D.P. Analysis of 15 adenovirus hexon proteins reveals the location and structure of seven hypervariable regions containing serotype-specific residues. Journal of Virology 1996, 70:1836-1844.
36. Sun Y., Li N., Li H.Y., Li M., Qiu H.J. Enhanced immunity against classical swine fever in pigs induced by prime-boost immunization using an alphavirus replicon-vectored DNA vaccine and a recombinant adenovirus. Veterinary Immunology and Immunopathology 2010, 137:20-27.
37. Hammond J.M., Jansen E.S., Morrissy C.J., Goff W.V., Meehan G.C., Williamson M.M., Lenghaus C., Sproat K.W., Andrew M.E., Coupar B.E., Johnson M.A. A prime-boost vaccination strategy using naked DNA followed by recombinant porcine adenovirus protects pigs from classical swine fever. Veterinary Microbiology 2001, 80:101-119.
38. Lakhashe S.K., Velu V., Sciaranghella G., Siddappa N.B., Dipasquale J.M., Hemashettar G., Yoon J.K., Rasmussen R.A., Yang F., Lee S.J., Montefiori D.C., Novembre F.J., Villinge R.F., Amara R.R., Kahn M., Hu S.L., Li S., Li Z., Frankel F.R., Robert-Gurof F.M., Johnson W.E., Lieberman J., Ruprecht R.M. Prime-boost vaccination with heterologous live vectors encoding SIV gag and multimeric HIV-1 gp160 protein: efficacy against repeated mucosal R5 clade C SHIV challenges. Vaccine 2011, 29:5611-5622.
39. Goodman A.L., Epp C., Moss D., Holder A.A., Wilson J.M., Gao G.P., Long C.A., Remarque E.J., Thomas A.W., Ammendola V., Colloca S., Dicks M.D., Biswas S., Seibel D., Van Duivenvoorde L.M., Gilbert S.C., Hill A.V., Draper S.J. New candidate vaccines against blood-stage Plasmodium falciparum malaria: prime-boost immunization regimens incorporating human and simian adenoviral vectors and poxviral vectors expressing an optimized antigen based on merozoite surface protein 1. Infection and Immunity 2010, 78:4601-4612.
40. Marshall E. Gene therapy death prompts review of adenovirus vector. Science 1999, 286(5448):2244-2245.
41. Józkowicz A., Dulak J. Helper-dependent adenoviral vectors in experimental gene therapy. Acta Biochimica Polonica 2005, 52(3):589-599.