Comparison of lymphocyte isolation methods for endoscopic biopsy specimens from the colonic mucosa

Journal of Immunological Methods

Volumn 389, Issue 1-2, 2013, Pages 29-37

  Carrasco, Anna ^a,e   Mañe, Josep ^b,e   Santaolalla, Rebeca ^a,e   Pedrosa, Elisabet ^b,e   Mallolas, Josefina ^a   Lorén, Violeta ^b,e   Fernández, Marco ^c   Fernández Bañares, Fernando ^a,e   Rosinach, Mercè ^a,e   Loras, Carme ^a,e   Forné, Montserrat ^a,e   Andújar, Xavier ^a   Vidal, Judith ^d   Viver, Josep Maria ^a,e   Esteve, Maria ^a,e  

^a UNIVERSITY OF BARCELONA   (Spain)

^b HOSPITAL UNIVERSITARI GERMANS TRIAS I PUJOL   (Spain)

^c FUNDACIÓ INSTITUT D INVESTIGACIÓ EN CIÈNCIES DE LA SALUT GERMANS TRIAS I PUJOL   (Spain)

^d Catlab   (Spain)

^e INSTITUTO DE SALUD CARLOS III   (Spain)

Author keywords

Colonic biopsies; Epithelium removal; Intraepithelial lymphocytes; Isolation methods; Lamina propria lymphocytes

Indexed keywords

CD103 ANTIGEN; CD16 ANTIGEN; CD19 ANTIGEN; CD3 ANTIGEN; CD4 ANTIGEN; CD45 ANTIGEN; CD8 ANTIGEN; COLLAGENASE;

ACCURACY; ADULT; ANTIGEN EXPRESSION; ARTICLE; CD4+ T LYMPHOCYTE; CD8+ T LYMPHOCYTE; CELL ISOLATION; CELL VIABILITY; COLON BIOPSY; COLON MUCOSA; CULTURE MEDIUM; ENDOSCOPIC BIOPSY; FLOW CYTOMETRY; HUMAN; HUMAN CELL; HUMAN CELL CULTURE; HUMAN EXPERIMENT; HUMAN TISSUE; IMMUNOHISTOCHEMISTRY; INTERMETHOD COMPARISON; INTESTINE CELL; LAMINA PROPRIA; LYMPHOCYTE; LYMPHOCYTE SUBPOPULATION; NORMAL HUMAN; PHENOTYPE; PRIORITY JOURNAL;

BIOPSY; CELL SEPARATION; CELL SURVIVAL; COLON; FLOW CYTOMETRY; HUMANS; IMMUNOPHENOTYPING; INTESTINAL DISEASES; INTESTINAL MUCOSA; LYMPHOCYTES;

EID: 84873709504     PISSN: 00221759     EISSN: 18727905     Source Type: Journal    
DOI: 10.1016/j.jim.2012.12.006     Document Type: Article

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