Chemical agents and enzymes used for the extraction of gut lymphocytes influence flow cytometric detection of T cell surface markers

Journal of Immunological Methods

Volumn 236, Issue 1-2, 2000, Pages 27-35

  Van Damme, Nancy ^a   Baeten, Dominique ^a   De Vos, Martine ^b   Demetter, Pieter ^b   Elewaut, Dirk ^a   Mielants, Herman ^a   Verbruggen, Gust ^a   Cuvelier, Claude ^b   Veys, Eric M ^a   De Keyser, Filip ^a  

^a GHENT UNIVERSITY   (Belgium)

^b GHENT UNIVERSITY HOSPITAL   (Belgium)

Author keywords

Collagenase; DTT; EDTA; Flow cytometry; Peripheral blood lymphocytes; Tonsillar lymphocytes

Indexed keywords

CD38 ANTIGEN; CD4 ANTIGEN; CD8 ANTIGEN; CLOSTRIDIOPEPTIDASE A; COLLAGENASE; DEOXYRIBONUCLEASE; DITHIOTHREITOL; EDETIC ACID; HERMES ANTIGEN; INTERLEUKIN 2 RECEPTOR; L SELECTIN; LYMPHOCYTE SURFACE MARKER; MONOCLONAL ANTIBODY;

ADOLESCENT; ADULT; ANTIGEN EXPRESSION; ARTICLE; CELL ISOLATION; CELL SUSPENSION; CHILD; CONTROLLED STUDY; FLOW CYTOMETRY; HUMAN; HUMAN CELL; IMMUNOFLUORESCENCE; INTESTINE; PERIPHERAL LYMPHOCYTE; PRIORITY JOURNAL; SAMPLE; T LYMPHOCYTE; TECHNIQUE; TISSUE; TONSIL;

ADOLESCENT; ADULT; ANTIGENS, CD; CELL MEMBRANE; CELL SEPARATION; CHILD; CHILD, PRESCHOOL; COLLAGENASES; DEOXYRIBONUCLEASES; DITHIOTHREITOL; EDETIC ACID; FLOW CYTOMETRY; HUMANS; INDICATORS AND REAGENTS; INTEGRINS; L-SELECTIN; MIDDLE AGED; T-LYMPHOCYTES; TONSIL;

EID: 0033951843     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-1759(99)00243-4     Document Type: Article

References (29)

1. Abuzakouk M., Feighery C., O'Farrelly C. Collagenase and dispase enzymes disrupt lymphocyte surface molecules. J. Immunol. Methods. 194:1996;211.
2. Adams D.H., Shaw S. Leucocyte-endothelial interactions and regulation of leucocyte migration. Lancet. 343:1994;831.
3. Balzano A., Bove A., Grande G., Bevilacqua N. Flow cytometric analysis of lymphocytes isolated from endoscopic biopsies in ulcerative colitis. Eur. J. Gastroenterol. Hepatol. 6:1994;203.
4. Bartnik W., Remine S.G., Chiba M., Thayer W.R., Shorter R.G. Isolation and characterization of colonic intraepithelial and lamina proprial lymphocytes. Gastroenterology. 78:1980;976.
5. Berg E.L., Robinson M.K., Warnock R.A., Butcher E.C. The human peripheral lymph node vascular addressin is a ligand for LECAM-1, the peripheral lymph node homing receptor. J. Cell. Biol. 114:1991;343.
6. Berlin C., Berg E.L., Briskin M.J., Andrew D.P., Kilshaw P.J., Holzmann B., Weissman I.L., Hamann A., Butcher E.C. Alpha 4 beta 7 integrin mediates lymphocyte binding to the mucosal vascular addressin MAdCAM-1. Cell. 74:1993;185.
7. Bevilacqua M.P. Endothelial-leukocyte adhesion molecules. Ann. Rev. Immunol. 11:1993;767.
8. Bull D.M., Bookman M.A. Isolation and functional characterization of human intestinal mucosal lymphoid cells. J. Clin. Invest. 59:1977;966.
9. Chiba M., Bartnik W., Remine S.G., Thayer W.R., Shorter R.G. Human colonic intraepithelial and lamina proprial lymphocytes: cytotoxity in vitro and the potential effects of the isolation method on their functional properties. Gut. 22:1981;177.
10. Haynes B.F., Telen M.J., Hale L.P., Denning S.M. CD44 - a molecule involved in leukocyte adherence and T-cell activation. Immunol. Today. 10:1989;423.
11. Hirata I., Berrebi G., Austin L.L., Keren D.F., Dobbins III W.O. Immunohistological characterization of intraepithelial and lamina propria lymphocytes in control ileum and colon and in inflammatory bowel disease. Dig. Dis. Sci. 31:1986;593.
12. Hynes R.O. Integrins: versatility, modulation, and signaling in cell adhesion. Cell. 69:1992;11.
13. Lynch S., Kelleher D., Feighery C., Weir D.G., O'Farrelly C. Flow cytometric analysis of intraepithelial lymphocytes from human small intestinal biopsies reveals populations of CD4+CD8+ and CD8αα+ cells. Eur. J. Gastroenterol. Hepatol. 5:1993;907.
14. Lyyra T., Arokoski J.P.A., Oksala N., Vihko A., Hyttinen M., Jurvelin J.S., Kiviranta I. Experimental validation of arthroscopic cartilage stifness measurement using enzymatically degraded cartilage samples. Phys. Med. Biol. 44:1999;525.
15. Maly F.-E., Nakamura M., Gauchat J.-F., Urwyler A., Walker C., Dahinden C.A., Cross A.R., Jones O.T.G., de Weck A.L. Superoxide-dependent nitroblue tetrazolium reduction and expression of cytochrome b-245 components by human tonsillar B lymphocytes and B cell lines. J. Immunol. 142:1989;1260.
16. Meenan J., Spaans J., Grool T.A., Pals S.T., Tytgat G.N.J., van Deventer S.J.H. Altered expression of α4β7, a gut homing integrin, by circulating and mucosal T cells in colonic mucosal inflammation. Gut. 40:1997;241.
17. Morgan J.M., Navabi H., Schmid K.W., Jasani B. Possible role of tissue-bound calcium ions in citrate-mediated high-temperature antigen retrieval. J. Pathol. 174:1994;301.
18. Picker L.J. Lymphocyte homing. Curr. Opin. Immunol. 4:1992;227.
19. Picker L.J. Control of lymphocyte homing. Curr. Opin. Immunol. 6:1994;394.
20. Qiu D., Cheng Tan W. Dithiothreitol has a dose-response effect on cell surface antigen expression. J. Allergy Clin. Immunol. 103:1999;873.
21. Rüthlein J., Heinze G., Auer I.O. Anti-CD2 and anti-CD3 induced T cell cytotoxicity of human intraepithelial and lamina propria lymphocytes. Gut. 33:1992;1626.
22. Selby W.S., Janossy G., Bofill M., Jewell D.P. Intestinal lymphoycte subpopulations in inflammatory bowel disease: an analysis by immunohistological and cell isolation techniques. Gut. 25:1984;32.
23. Senaldi G., Lobo-Yeo A., Mowat A.P., Mieli-Vergani G., Vergani D. Class I and class II major histocompatibility complex antigens on hepatocytes: Importance of the method of detection and expression in histologically normal and diseased livers. J. Clin. Pathol. 44:1991;107.
24. Senju M., Wu K.C., Mahida Y.R., Jewell D.P. Two-color immunofluorescence and flow cytometric analysis of lamina propria lymphoycte subsets in Ulcerative colitis and Crohn's disease. Dig. Dis. Sci. 36:1990;1453.
25. Shi S.-R., Cote R.J., Hawes D., Thu S., Shi Y., Young L.L., Taylor C.R. Calcium-induced modification of protein conformation demonstrated by immunohistochemistry: what is the signal? J. Histochem. Cytochem. 47:1999;463.
26. Smart C.J., Trejdosiewicz L.K., Badr-El-Din S., Heatley R.V. T lymphocytes of the human colonic mucosa: functional and phenotypic analysis. Clin. Exp. Immunol. 73:1988;63.
27. Strauch U.G., Lifka A., Gosslar U., Kilshaw P.J., Clements J., Holzmann B. Distinct binding specificities of integrins alpha 4 beta 7 (LPAM-1), alpha 4 beta 1 (VLA-4), and alpha IEL beta 7. Int. Immunol. 6:1994;263.
28. Zheng M., Fang H., Hakomori S. Functional role of N-glycosylation in α5β1 integrin receptor. J. Biol. Chem. 269:1994;12325.
29. Zola H., Fusco M., Ridings J., Flego L.R., Weedon H.M., Nicholson I., Organ N., Roberton D.M., Macardle P.J. The Fas antigen (CD95) on lymphoid cells: epitope analysis with ten antibodies. Tissue Antigens. 48:1996;519.