Microparticle counts in platelet-rich and platelet-free plasma, effect of centrifugation and sample-processing protocols

Blood Coagulation and Fibrinolysis

Volumn 24, Issue 2, 2013, Pages 125-132

  Chandler, Wayne L ^a  

^a HOUSTON METHODIST HOSPITAL   (United States)

Author keywords

annexin V; centrifugation; microparticles; platelet rich plasma

Indexed keywords

ANNEXIN; FIBRINOGEN RECEPTOR;

ADULT; ARTICLE; CENTRIFUGATION; CONTROLLED STUDY; ELECTRIC POTENTIAL; FEMALE; FLOW CYTOMETRY; FREEZE THAWING; HUMAN; MALE; NORMAL HUMAN; PLATELET MICROPARTICLE; PRIORITY JOURNAL; THROMBOCYTE FREE PLASMA; THROMBOCYTE RICH PLASMA;

ADULT; ANNEXIN A5; CELL-DERIVED MICROPARTICLES; CENTRIFUGATION; FEMALE; FLOW CYTOMETRY; HUMANS; MALE; MIDDLE AGED; PLATELET-RICH PLASMA;

EID: 84873407266     PISSN: 09575235     EISSN: 14735733     Source Type: Journal    
DOI: 10.1097/MBC.0b013e32835a0824     Document Type: Article

References (31)

1. Freyssinet JM, Toti F. Formation of procoagulant microparticles and properties. Thromb Res 2010; 125 (SUPPL1):S46-S48
2. Lacroix R, Robert S, Poncelet P, Dignat-George F. Overcoming limitations of microparticle measurement by flow cytometry. Semin Thromb Hemost 2010; 36:807-818
3. Bidot L, Jy W, Bidot C Jr, Jimenez JJ, Fontana V, Horstman LL, et al. Microparticle-mediated thrombin generation assay: Increased activity in patients with recurrent thrombosis. J Thromb Haemost 2008; 6:913-919
4. Shet AS, Aras O, Gupta K, Hass MJ, Rausch DJ, Saba N, et al. Sickle blood contains tissue factor-positive microparticles derived from endothelial cells and monocytes. Blood 2003; 102:2678-2683
5. Helal O, Defoort C, Robert S, Marin C, Lesavre N, Lopez-Miranda J, et al. Increased levels of microparticles originating from endothelial cells, platelets and erythrocytes in subjects with metabolic syndrome: Relationship with oxidative stress. Nutr Metab Cardiovasc Dis 2011; 21:665-671
6. Ayers L, Kohler M, Harrison P, Sargent I, Dragovic R, Schaap M, et al. Measurement of circulating cell-Derived microparticles by flow cytometry: Sources of variability within the assay. Thromb Res 2010; 127:370-377
7. Yuana Y, Bertina RM, Osanto S. Preanalytical and analytical issues in the analysis of blood microparticles. Thromb Haemost 2011; 105:396-408
8. Matzdorff AC, Kuhnel G, Kemkes-Matthes B, Pralle H. Quantitative assessment of platelets, platelet microparticles, and platelet aggregates with flow cytometry. J Lab Clin Med 1998; 131:507-517
9. Moles-Moreau MP, Ternisien C, Tanguy-Schmidt A, Boyer F, Gardembas M, Dib M, et al. Flow cytometry-Evaluated platelet CD36 expression, reticulated platelets and platelet microparticles in essential thrombocythaemia and secondary thrombocytosis. Thromb Res 2010; 126:e394-E396
10. Simak J, Holada K, Risitano AM, Zivny JH, Young NS, Vostal JG. Elevated circulating endothelial membrane microparticles in paroxysmal nocturnal haemoglobinuria. Br J Haematol 2004; 125:804-813
11. Robert S, Poncelet P, Lacroix R, Arnaud L, Giraudo L, Hauchard A, et al. Standardization of platelet-Derived microparticle counting using calibrated beads and a Cytomics FC500 routine flow cytometer: A first step towards multicenter studies? J Thromb Haemost 2009; 7:190-197
12. Kim HK, Song KS, Lee ES, Lee YJ, Park YS, Lee KR, et al. Optimized flow cytometric assay for the measurement of platelet microparticles in plasma: Preanalytic and analytic considerations. Blood Coagul Fibrinolysis 2002; 13:393-397
13. Berckmans RJ, Nieuwland R, Boing AN, Romijn FP, Hack CE, Sturk A. Cell-Derived microparticles circulate in healthy humans and support low grade thrombin generation. Thromb Haemost 2001; 85:639-646
14. Connor DE, Exner T, Ma DD, Joseph JE. The majority of circulating plateletderived microparticles fail to bind annexin V, lack phospholipid- Dependent procoagulant activity and demonstrate greater expression of glycoprotein Ib. Thromb Haemost 2010; 103:1044-1052
15. Yuana Y, Oosterkamp TH, Bahatyrova S, Ashcroft B, Garcia Rodriguez P, Bertina RM, et al. Atomic force microscopy: A novel approach to the detection of nanosized blood microparticles. J Thromb Haemost 2009; 8:315-323
16. Horstman LL, Jy W, Jimenez JJ, Bidot C, Ahn YS. New horizons in the analysis of circulating cell-Derived microparticles. Keio J Med 2004; 53:210-230
17. van Ierssel SH, Van Craenenbroeck EM, Conraads VM, Van Tendeloo VF, Vrints CJ, Jorens PG, et al. Flow cytometric detection of endothelial microparticles (EMP): Effects of centrifugation and storage alter with the phenotype studied. Thromb Res 2010; 125:332-339
18. Biro E, Sturk-Maquelin KN, Vogel GM, Meuleman DG, Smit MJ, Hack CE, et al. Human cell-Derived microparticles promote thrombus formation in vivo in a tissue factor-Dependent manner. J Thromb Haemost 2003; 1:2561-2568
19. Foladori P, Quaranta A, Ziglio G. Use of silica microspheres having refractive index similar to bacteria for conversion of flow cytometric forward light scatter into biovolume. Water Res 2008; 42:3757-3766
20. Koch AL, Robertson BR, Button DK. Deduction of the cell volume and mass from forward scatter intensity of bacteria analyzed by flow cytometry. J Microbiol Methods 1996; 27:49-61
21. Chandler WL, Yeung W, Tait JF. A new microparticle size calibration standard for use in measuring smaller microparticles using a new flow cytometer. J Thromb Haemost 2011; 9:1216-1224
22. Zwicker JI, Liebman HA, Neuberg D, Lacroix R, Bauer KA, Furie BC, et al. Tumor-Derived tissue factor-bearing microparticles are associated with venous thromboembolic events in malignancy. Clin Cancer Res 2009; 15:6830-6840
23. Macey MG, Bevan S, Alam S, Verghese L, Agrawal S, Beski S, et al. Platelet activation and endogenous thrombin potential in preeclampsia. Thromb Res 2010; 125:e76-E81
24. Lacroix R, Robert S, Poncelet P, Kasthuri RS, Key NS, Dignat-George F. Standardization of platelet-Derived microparticle enumeration by flow cytometry using calibrated beads: Results of ISTH SSC collaborative workshop. J Thromb Haemost 2010; 8:2571-2574
25. Macey MG, Enniks N, Bevan S. Flow cytometric analysis of microparticle phenotype and their role in thrombin generation. Cytometry B Clin Cytom 2011; 80:57-63
26. Rubin O, Crettaz D, Tissot JD, Lion N. Preanalytical and methodological challenges in red blood cell microparticle proteomics. Talanta 2010; 82:1-8
27. Exner T, Joseph JE, Connor D, Low J, Ma DD. Increased procoagulant phospholipid activity in blood from patients with suspected acute coronary syndromes: A pilot study. Blood Coagul Fibrinolysis 2005; 16:375-379
28. Dean WL, Lee MJ, Cummins TD, Schultz DJ, Powell DW. Proteomic and functional characterisation of platelet microparticle size classes. Thromb Haemost 2009; 102:711-718
29. Dey-Hazra E, Hertel B, Kirsch T, Woywodt A, Lovric S, Haller H, et al. Detection of circulating microparticles by flow cytometry: Influence of centrifugation, filtration of buffer, and freezing. Vasc Health Risk Manag 2010; 6:1125-1133
30. Mobarrez F, Antovic J, Egberg N, Hansson M, Jorneskog G, Hultenby K, et al. A multicolor flow cytometric assay for measurement of platelet-Derived microparticles. Thromb Res 2009; 125:e110-E116
31. Bohling SD, Pagano MB, Stitzel MR, Ferrell C, Yeung W, Chandler WL. Comparison of clot-based versus chromogenic factor Xa procoagulant phospholipid activity assays. Am J Clin Pathol 2012; 137:185-192