Studies of pH-dependent self-association of a recombinant form of arylsulfatase a with electrospray ionization mass spectrometry and size-exclusion chromatography

Analytical Chemistry

Volumn 85, Issue 3, 2013, Pages 1591-1596

  Abzalimov, Rinat R ^a   Bobst, Cedric E ^a   Salinas, Paul A ^b   Savickas, Philip ^b   Thomas, John J ^b   Kaltashov, Igor A ^a  

^a UNIVERSITY OF MASSACHUSETTS   (United States)

^b Shire Human Genetic Therapies   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACIDIC ENVIRONMENT; ALKALINE PH; ARYLSULFATASE; ASSEMBLY PROCESS; COOPERATIVE PROCESS; ELECTROSPRAY IONIZATION MASS SPECTROMETRY; ENDOGENOUS ENZYME; ENZYME REPLACEMENT THERAPY; HEXAMERS; HOMODIMERS; INTERCONVERSIONS; INTERMEDIATE SPECIE; INTERMEDIATE STATE; NEURODEGENERATIVE; NEUTRAL PH; OCTAMERS; PERIPHERAL NERVOUS SYSTEM; PH RANGE; PH-DEPENDENT; PROTEIN CONCENTRATIONS; RETENTION TIME; SELF-ASSOCIATIONS; SOLUTION PH; TETRAMERS; TIME-SCALES; TRANSITION STATE;

ASSOCIATION REACTIONS; CATALYST ACTIVITY; CELL MEMBRANES; DIMERS; ENZYMES; MASS SPECTROMETRY; NEURODEGENERATIVE DISEASES; OLIGOMERIZATION; PROTEINS; SIZE EXCLUSION CHROMATOGRAPHY;

ALKALINITY;

CEREBROSIDE SULFATASE; RECOMBINANT PROTEIN;

ARTICLE; CHEMISTRY; ELECTROSPRAY MASS SPECTROMETRY; GEL CHROMATOGRAPHY; HUMAN; METHODOLOGY; PH;

CEREBROSIDE-SULFATASE; CHROMATOGRAPHY, GEL; HUMANS; HYDROGEN-ION CONCENTRATION; RECOMBINANT PROTEINS; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION;

EID: 84873333987     PISSN: 00032700     EISSN: 15206882     Source Type: Journal    
DOI: 10.1021/ac302829k     Document Type: Article

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