The effects of an RGD-PAMAM dendrimer conjugate in 3D spheroid culture on cell proliferation, expression and aggregation

Biomaterials

Volumn 34, Issue 11, 2013, Pages 2665-2673

  Jiang, Li Yang ^a   Lv, Bing ^a   Luo, Ying ^a  

^a PEKING UNIVERSITY   (China)

Author keywords

Dendrimer; Multicellular aggregation; Multivalent; Peptide ligand

Indexed keywords

3-D CELL CULTURE; 3D CULTURE; BINDING BEHAVIORS; BIO-MOLECULAR; CELLULAR MICROENVIRONMENT; CELLULAR PROLIFERATIONS; CULTURE SYSTEMS; EXTRACELLULAR; HIGH DENSITY; MEMBRANE RECEPTORS; MRNA EXPRESSION; MULTICELLULAR SPHEROID; MULTICELLULAR SYSTEMS; MULTIVALENT; PEPTIDE LIGAND; POLYAMIDOAMINES; SPATIAL ORGANIZATION;

CELL CULTURE; CELL PROLIFERATION; CELLS; DENDRIMERS; ENZYME ACTIVITY; PEPTIDES; THREE DIMENSIONAL COMPUTER GRAPHICS; TISSUE ENGINEERING;

LIGANDS;

ARGINYLGLYCYLASPARTIC ACID; ARGINYLGLYCYLASPARTIC ACID POLYAMIDOAMINE DENDRIMER CONJUGATE; DENDRIMER; MESSENGER RNA; POLYAMIDOAMINE; UNCLASSIFIED DRUG;

ARTICLE; CELL AGGREGATION; CELL CULTURE; CELL PROLIFERATION; CELL STRAIN 3T3; CONTROLLED STUDY; DRUG ACTIVITY; GENE EXPRESSION; PRIORITY JOURNAL; PROTEIN MODIFICATION; SPHEROID CELL;

ANIMALS; CELL CULTURE TECHNIQUES; CELL PROLIFERATION; DENDRIMERS; GENE EXPRESSION; LIGANDS; MICE; MOLECULAR STRUCTURE; NIH 3T3 CELLS; NYLONS; OLIGOPEPTIDES; POLYAMINES; REAL-TIME POLYMERASE CHAIN REACTION; RNA, MESSENGER; SPHEROIDS, CELLULAR; TISSUE ENGINEERING;

EID: 84873083026     PISSN: 01429612     EISSN: 18785905     Source Type: Journal    
DOI: 10.1016/j.biomaterials.2013.01.003     Document Type: Article

References (44)

1. Tomalia D.A., Baker H., Dewald J., Hall M., Kallos G., Martin S., et al. A new class of polymers: starburst-dendritic macromolecules. Polym J 1985, 17:117-132.
2. Tomalia D.A., Naylor A.M., Goddard W.A. Starburst dendrimers: molecular-level control of size, shape, surface chemistry, topology, and flexibility from atoms to macroscopic matter. Angew Chem Int Ed Engl 1990, 29:138-175.
3. Tomalia D.A. Dendrimer research. Science 1991, 252:1231.
4. Wolfenden M.L., Cloninger M.J. Mannose/glucose-functionalized dendrimers to investigate the predictable tunability of multivalent interactions. J Am Chem Soc 2005, 127:12168-12169.
5. Higashiyama S., Noda M., Kawase M., Yagi K. Mixed-ligand modification of polyamidoamine dendrimers to develop an effective scaffold for maintenance of hepatocyte spheroids. J Biomed Mater Res A 2003, 64:475-482.
6. Fasting C., Schalley C.A., Weber M., Seitz O., Hecht S., Koksch B., et al. Multivalency as a chemical organization and action principle. Angew Chem Int Ed Engl 2012, 51:10472-10498.
7. Barnard A., Smith D.K. Self-assembled multivalency: dynamic ligand arrays for high-affinity binding. Angew Chem Int Ed Engl 2012, 51:6572-6581.
8. Zanini D., Roy R. Chemoenzymatic synthesis and lectin binding properties of dendritic N-acetyllactosamine. Bioconjug Chem 1997, 8:187-192.
9. Shukla R., Thomas T.P., Peters J., Kotlyar A., Myc A., Baker J.R. Tumor angiogenic vasculature targeting with PAMAM dendrimer-RGD conjugates. Chem Commun (Camb) 2005, 46:5739-5741.
10. Hill E., Shukla R., Park S.S., Baker J.R. Synthetic PAMAM-RGD conjugates target and bind to odontoblast-like MDPC 23 cells and the predentin in tooth organ cultures. Bioconjug Chem 2007, 18:1756-1762.
11. McNerny D.Q., Kukowska-Latallo J.F., Mullen D.G., Wallace J.M., Desai A.M., Shukla R., et al. RGD dendron bodies; synthetic avidity agents with defined and potentially interchangeable effector sites that can substitute for antibodies. Bioconjug Chem 2009, 20:1853-1859.
12. Higuchi Y., Wu C., Chang K.L., Irie K., Kawakami S., Yamashita F., et al. Polyamidoamine dendrimer-conjugated quantum dots for efficient labeling of primary cultured mesenchymal stem cells. Biomaterials 2011, 32:6676-6682.
13. Yuan Q., Lee E., Yeudall W.A., Yang H. Dendrimer-triglycine-EGF nanoparticles for tumor imaging and targeted nucleic acid and drug delivery. Oral Oncol 2010, 46:698-704.
14. Choi J.S., Nam K., Park J.Y., Kim J.B., Lee J.K., Park J.S. Enhanced transfection efficiency of PAMAM dendrimer by surface modification with L-arginine. J Control Release 2004, 99:445-456.
15. Pandita D., Santos J.L., Rodrigues J., Pego A.P., Granja P.L., Tomas H. Gene delivery into mesenchymal stem cells: a biomimetic approach using RGD nanoclusters based on poly(amidoamine) dendrimers. Biomacromolecules 2011, 12:472-486.
16. Yang H., Kao W.J. Synthesis and characterization of nanoscale dendritic RGD clusters for potential applications in tissue engineering and drug delivery. Int J Nanomedicine 2007, 2:89-99.
17. Arnold M., Cavalcanti-Adam E.A., Glass R., Blummel J., Eck W., Kantlehner M., et al. Activation of integrin function by nanopatterned adhesive interfaces. Chem Phys Chem 2004, 5:383-388.
18. Li J.R., Shi L., Deng Z., Lo S.H., Liu G.Y. Nanostructures of designed geometry and functionality enable regulation of cellular signaling processes. Biochemistry 2012, 51:5876-5893.
19. Kelm J.M., Fussenegger M. Scaffold-free cell delivery for use in regenerative medicine. Adv Drug Deliv Rev 2010, 62:753-764.
20. Pampaloni F., Reynaud E.G., Stelzer E.H. The third dimension bridges the gap between cell culture and live tissue. Nat Rev Mol Cell Biol 2007, 8:839-845.
21. Lin R.Z., Chang H.Y. Recent advances in three-dimensional multicellular spheroid culture for biomedical research. Biotechnol J 2008, 3:1172-1184.
22. Abu-Absi S.F., Friend J.R., Hansen L.K., Hu W.S. Structural polarity and functional bile canaliculi in rat hepatocyte spheroids. Exp Cell Res 2002, 274:56-67.
23. Khetani S.R., Bhatia S.N. Microscale culture of human liver cells for drug development. Nat Biotechnol 2008, 26:120-126.
24. Hwang Y.S., Chung B.G., Ortmann D., Hattori N., Moeller H.C., Khademhosseini A. Microwell-mediated control of embryoid body size regulates embryonic stem cell fate via differential expression of WNT5a and WNT11. Proc Natl Acad Sci U S A 2009, 106:16978-16983.
25. Baraniak P.R., McDevitt T.C. Scaffold-free culture of mesenchymal stem cell spheroids in suspension preserves multilineage potential. Cell Tissue Res 2012, 347:701-711.
26. Maheshwari G., Brown G., Lauffenburger D.A., Wells A., Griffith L.G. Cell adhesion and motility depend on nanoscale RGD clustering. J Cell Sci 2000, 113:1677-1686.
27. Boswell C.A., Eck P.K., Regino C.A., Bernardo M., Wong K.J., Milenic D.E., et al. Synthesis, characterization, and biological evaluation of integrin alphavbeta3-targeted PAMAM dendrimers. Mol Pharmacol 2008, 5:527-539.
28. Waite C.L., Roth C.M. PAMAM-RGD conjugates enhance siRNA delivery through a multicellular spheroid model of malignant glioma. Bioconjug Chem 2009, 20:1908-1916.
29. Massia S.P., Hubbell J.A. An RGD spacing of 440 nm is sufficient for integrin alpha V beta 3-mediated fibroblast spreading and 140 nm for focal contact and stress fiber formation. J Cell Biol 1991, 114:1089-1100.
30. Hersel U., Dahmen C., Kessler H. RGD modified polymers: biomaterials for stimulated cell adhesion and beyond. Biomaterials 2003, 24:4385-4415.
31. Dai W., Belt J., Saltzman W.M. Cell-binding peptides conjugated to poly(ethylene glycol) promote neural cell aggregation. Biotechnology (NY) 1994, 12:797-801.
32. Dai W., Saltzman W.M. Fibroblast aggregation by suspension with conjugates of poly(ethylene glycol) and RGD. Biotechnol Bioeng 1996, 50:349-356.
33. Kim J.K., Shukla R., Casagrande L., Sedgley C., Nor J.E., Baker J.R., et al. Differentiating dental pulp cells via RGD-dendrimer conjugates. J Dent Res 2010, 89:1433-1438.
34. Geiger B., Spatz J.P., Bershadsky A.D. Environmental sensing through focal adhesions. Nat Rev Mol Cell Biol 2009, 10:21-33.
35. Sevilla C.A., Dalecki D., Hocking D.C. Extracellular matrix fibronectin stimulates the self-assembly of microtissues on native collagen gels. Tissue Eng Part A 2010, 16:3805-3819.
36. Kelm J.M., Lorber V., Snedeker J.G., Schmidt D., Broggini-Tenzer A., Weisstanner M., et al. A novel concept for scaffold-free vessel tissue engineering: self-assembly of microtissue building blocks. J Biotechnol 2010, 148:46-55.
37. Urciuolo F., Imparato G., Palmiero C., Trilli A., Netti P.A. Effect of process conditions on the growth of three-dimensional dermal-equivalent tissue obtained by microtissue precursor assembly. Tissue Eng Part C, Methods 2011, 17:155-164.
38. Lee C.C., MacKay J.A., Frechet J.M., Szoka F.C. Designing dendrimers for biological applications. Nat Biotechnol 2005, 23:1517-1526.
39. Bao B., Jiang J., Yanase T., Nishi Y., Morgan J.R. Connexon-mediated cell adhesion drives microtissue self-assembly. FASEB J 2011, 25:255-264.
40. Ong S.M., He L., Thuy Linh N.T., Tee Y.H., Arooz T., Tang G., et al. Transient inter-cellular polymeric linker. Biomaterials 2007, 28:3656-3667.
41. Zhao D., Ong S.M., Yue Z., Jiang Z., Toh Y.C., Khan M., et al. Dendrimer hydrazides as multivalent transient inter-cellular linkers. Biomaterials 2008, 29:3693-3702.
42. Gartner Z.J., Bertozzi C.R. Programmed assembly of 3dimensional microtissues with defined cellular connectivity. Proc Natl Acad Sci U S A 2009, 106:4606-4610.
43. Gestwicki J.E., Strong L.E., Cairo C.W., Boehm F.J., Kiessling L.L. Cell aggregation by scaffolded receptor clusters. Chem Biol 2002, 9:163-169.
44. Yu X., Liu Z., Janzen J., Chafeeva I., Horte S., Chen W., et al. Polyvalent choline phosphate as a universal biomembrane adhesive. Nat Mater 2012, 11:468-476.