Improved thermostability of lipase b from candida antarctica by directed evolution and display on yeast surface

Applied Biochemistry and Biotechnology

Volumn 169, Issue 2, 2013, Pages 351-358

  Peng, Xiang Qian ^a  

^a LIAOCHENG UNIVERSITY   (China)

Author keywords

Candida antarctica lipase B; Multi site saturation mutagenesis; Thermostability; Yeast cell surface display

Indexed keywords

AMINO ACID SUBSTITUTION; AQUEOUS SYSTEM; CANDIDA ANTARCTICA LIPASE B; DIRECTED EVOLUTION; LIPASE B FROM CANDIDA ANTARCTICA; MULTI-SITE; RESIDUAL ACTIVITY; SATURATION MUTAGENESIS; SURFACE DISPLAYS; SURFACE DOMAINS; THERMOSTABILITY; YEAST CELL SURFACE;

AMINO ACIDS; CELL MEMBRANES; HYDROGEN BONDS; ISOMERS; MUTAGENESIS;

YEAST;

AMINO ACID; GENOMIC DNA; LIPASE B;

AMINO ACID SUBSTITUTION; AQUEOUS SOLUTION; ARTICLE; CANDIDA ANTARCTICA; CELL SURFACE DISPLAY; CELL TRANSFORMATION; DNA SEQUENCE; ENZYME STABILITY; FACTORIAL ANALYSIS; GENE EXPRESSION SYSTEM; HYDROGEN BOND; MUTAGENESIS; MUTANT; NONHUMAN; PICHIA PASTORIS; PREDICTION; WILD TYPE; YEAST CELL;

CELL MEMBRANE; DIRECTED MOLECULAR EVOLUTION; ENZYME ACTIVATION; ENZYME STABILITY; FUNGAL PROTEINS; LIPASE; PROTEIN ENGINEERING; SACCHAROMYCES CEREVISIAE; TEMPERATURE;

CANDIDA ANTARCTICA;

EID: 84873080431     PISSN: 02732289     EISSN: 15590291     Source Type: Journal    
DOI: 10.1007/s12010-012-9954-7     Document Type: Article

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