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Volumn 23, Issue 4, 2013, Pages 1075-1078

Mitochondrial dysfunction contributes to the cytotoxicity of some 3,5-bis(benzylidene)-4-piperidone derivatives in colon HCT-116 cells

Author keywords

Cytotoxicity; Glutathione; Mitochondria; Mitochondrial membrane potential; Reactive oxygen species

Indexed keywords

1 AROYL 3,5 BIS(BENZYLIDENE) 4 PIPERIDONE DERIVATIVE; CURCUMIN; GLUTATHIONE; GLUTATHIONE TRANSFERASE P1; PIPERIDONE DERIVATIVE; REACTIVE OXYGEN METABOLITE; UNCLASSIFIED DRUG;

EID: 84872972513     PISSN: 0960894X     EISSN: 14643405     Source Type: Journal    
DOI: 10.1016/j.bmcl.2012.12.016     Document Type: Article
Times cited : (11)

References (37)
  • 3
    • 73449111124 scopus 로고    scopus 로고
    • H. Kalant, D.M. Grant, J. Mitchell, 7th ed. Saunders-Elsevier Toronto
    • E.X. Chen, and M.J. Moore H. Kalant, D.M. Grant, J. Mitchell, Principles of Medical Pharmacology 7th ed. 2007 Saunders-Elsevier Toronto 778
    • (2007) Principles of Medical Pharmacology , pp. 778
    • Chen, E.X.1    Moore, M.J.2
  • 12
    • 84872894234 scopus 로고    scopus 로고
    • US Patent 7,582,655, September 1
    • Dimmock, J. R.; Das, U. US Patent 7,582,655, September 1, 2009.
    • (2009)
    • Dimmock, J.R.1    Das, U.2
  • 16
    • 84872933390 scopus 로고    scopus 로고
    • 17 In brief, HCT-116 and CRL-1790 cells were propagated on McCoy's and Minimum essential media, respectively. The concentrations of dimethylsulfoxide in these two media were 1% and 0.5%, respectively. Different concentrations of the compounds were added to the cells in the media and incubated for 48 h. Sulforhodamine B was used to monitor the change in cell number
    • 17 In brief, HCT-116 and CRL-1790 cells were propagated on McCoy's and Minimum essential media, respectively. The concentrations of dimethylsulfoxide in these two media were 1% and 0.5%, respectively. Different concentrations of the compounds were added to the cells in the media and incubated for 48 h. Sulforhodamine B was used to monitor the change in cell number.
  • 23
    • 84872916589 scopus 로고    scopus 로고
    • 24 In brief, HCT-116 cells were incubated with 2a (0.57 μM) 2b (0.50 μM) or 5-FU (3.5 μM) for 48 h. Dichlorodihydrofluorescein diacetate was added to the cells so that a final concentration of the dye was 5 μM. After incubating for 0.5 h and washing, the intracellular dye was excited using light of 483 nm and the emission was recorded at 530 nm
    • 24 In brief, HCT-116 cells were incubated with 2a (0.57 μM) 2b (0.50 μM) or 5-FU (3.5 μM) for 48 h. Dichlorodihydrofluorescein diacetate was added to the cells so that a final concentration of the dye was 5 μM. After incubating for 0.5 h and washing, the intracellular dye was excited using light of 483 nm and the emission was recorded at 530 nm.
  • 28
    • 84872901912 scopus 로고    scopus 로고
    • 29 In brief, HCT-116 cells were treated with 2a (0.70 μM), 2b (0.50 μM), CCCP (1 μM) or 2,4-dinitrophenol (1 μM) and then incubated for 48 h. The JC-1 dye was added to the cells and after 0.5 h excess dye was removed by successive washing of the cells with phosphate buffered saline. Fluorescence in the cells was measured at 535 nm after excitation with light of a wavelength of 485 nm.
    • 29 In brief, HCT-116 cells were treated with 2a (0.70 μM), 2b (0.50 μM), CCCP (1 μM) or 2,4-dinitrophenol (1 μM) and then incubated for 48 h. The JC-1 dye was added to the cells and after 0.5 h excess dye was removed by successive washing of the cells with phosphate buffered saline. Fluorescence in the cells was measured at 535 nm after excitation with light of a wavelength of 485 nm.
  • 31
    • 84872947058 scopus 로고    scopus 로고
    • 33 and at 1 mg protein/mL were incubated at 30 °C in an aqueous buffer pH 7.2 containing sucrose (125 mM), HEPES (10 mM), potassium phosphate (5 mM), magnesium chloride (1 mM) and succinate (5 mM). The data were obtained using light spectroscopy at 520 nm. Rats were handled and euthanized following the protocols established by the Canadian Council of Animal Care and administered by the University of Saskatchewan Committee on Animal Care and Supply
    • 33 and at 1 mg protein/mL were incubated at 30 °C in an aqueous buffer pH 7.2 containing sucrose (125 mM), HEPES (10 mM), potassium phosphate (5 mM), magnesium chloride (1 mM) and succinate (5 mM). The data were obtained using light spectroscopy at 520 nm. Rats were handled and euthanized following the protocols established by the Canadian Council of Animal Care and administered by the University of Saskatchewan Committee on Animal Care and Supply.


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