Spatiotemporal focusing-based widefield multiphoton microscopy for fast optical sectioning of thick tissues

Proceedings of SPIE - The International Society for Optical Engineering

Volumn 8520, Issue , 2012, Pages

  Cheng, Li Chung ^a   Chang, Chia Yuan ^a   Yen, Wei Chung ^b   Chen, Shean Jen ^a  

^a NATIONAL CHENG KUNG UNIVERSITY   (Taiwan)

^b CHUNG SHAN INSTITUTE OF SCIENCE AND TECHNOLOGY   (Taiwan)

Author keywords

Brownian motion; Second harmonic Generation; Spatiotemporal focusing; Widefield multiphoton microscopy

Indexed keywords

AXIAL RESOLUTIONS; BEAM SCANNING; BIO-IMAGING; ELECTRON MULTIPLYING CHARGE COUPLED DEVICES; FRAME RATE; FS PULSE; MICROBEADS; MULTI-PHOTON MICROSCOPY; MULTIPHOTON EXCITATION; MULTIPHOTONS; OPTICAL SECTIONING; PEAK POWER; REPETITION RATE; SCATTERING NOISE; SPATIAL RESOLUTION; SPATIOTEMPORAL FOCUSING; THICK-TISSUE; ULTRA-FAST; WIDE-FIELD;

BROWNIAN MOVEMENT; EXCITED STATES; HARMONIC GENERATION; NONLINEAR OPTICS; TISSUE;

FOCUSING;

EID: 84872907032     PISSN: 0277786X     EISSN: None     Source Type: Conference Proceeding    
DOI: 10.1117/12.930324     Document Type: Conference Paper

References (28)

1. W. R. Zipfel, R. M. Williams, and W. W. Webb, "Nonlinear magic: multiphoton microscopy in the bioscience," Nat. Biotechnol. 21, 1369-1377 (2003). (Pubitemid 37356624)
2. I. Freund and M. Deutsch, "Second-harmonic microscopy of biological tissue," Opt. Lett. 11, 94-96 (1986).
3. G. J. Brakenhoff, J. Squier, T. Norris, A. C. Bliton, M. H. Wade, and B. Athey, "Real-time two photon confocal microscopy using femtosecond, amplified Ti:sapphire system," J. microscopy 181, 253-259 (1996). (Pubitemid 26124656)
4. A. H. Buist, M. Müller, J. Squier, and G. J. Brakenhoff, "Real time two-photon absorption microscopy using multipoint excitation," J. microscopy 192, 217-226 (1998). (Pubitemid 28539626)
5. J. Bewersdorf, R. Pick, and S. W. Hell, "Multifocal multiphoton microscopy," Opt. Lett. 23, 655-657 (1998). (Pubitemid 128590668)
6. D. Oron, E. Tal, and Y. Silberberg, "Scanningless depth-resolved microscopy," Opt. Express 13, 1468-1476 (2005) (Pubitemid 40548703)
7. E. Tal, D. Oron, and Y. Silberberg, "Improved depth resolution in video-rate line-scanning multiphoton microscopy using temporal focusing," Opt. Lett. 30, 1686-1688 (2005). (Pubitemid 40972041)
8. M. E. Durst, G. Zhu, and C. Xu, "Simultaneous spatial and temporal focusing in nonlinear microscopy," Opt. Commun. 281, 1796-1805 (2008). (Pubitemid 351274126)
9. A. Vaziri, J. Tang, H. Shroff, and C. V. Shank, "Multilayer three-dimensional super resolution imaging of thick biological samples," PNAS 105, 20221-20226 (2008).
10. A. Vaziri and C. V. Shank, "Ultrafast widefield optical sectioning microscopy by multifocal temporal focusing," Opt. Express 18, 19645-19655 (2010).
11. E. Papagiakoumou, F. Anselmi, A. Bègue, V. deSars, J. Glückstad, E. Y. Isacoff, and V. Emiliani, "Scanless twophoton excitation of channelrhodopsin-2," Nat. Methods 7, 848-854 (2010).
12. O. D. Therrien, B. Aubé, S. Pagès, P. De Koninck, and D. Côté, "Wide-field multiphoton imaging of cellular dynamics in thick tissue by temporal focusing and patterned illumination," Biomed. Opt. Express 2, 696-704 (2011).
13. K. H. Kim, C. Buehler, and P. T. C. So, "High-speed, two-photon scanning microscope," Appl. Opt. 38, 6004- 6009 (1999). (Pubitemid 129569777)
14. A. Egner and S. W. Hell, "Time multiplexing and parallelization in multifocal multiphoton microscopy," J. Opt. Soc. Am. A 17, 1192-1201 (2000).
15. P. J. Keller and E. H. K. Stelzer, "Quantitative in vivo imaging of entire embryos with digital scanned laser light sheet fluorescence microscopy," Curr. Opin. Neurobiol. 18, 624-632 (2008).
16. J. Vermot, S. E. Fraser, and M. Liebling, "Fast fluorescence microscopy for imaging the dynamics of embryonic development," HFSP J. 2, 143-155 (2008).
17. J. R. Lakowicz, Principles of Fluorescence Spectroscopy (Springer, 2006).
18. A. V. Orden, N. P. Machara, P. M. Goodwin, and R. A. Keller, "Single-molecule identification in flowing sample streams by fluorescence burst size and intraburst fluorescence decay rate," Anal. Chem. 70, 1444-1451 (1998). (Pubitemid 128500445)
19. H. Dana and S. Shoham, "Numerical evaluation of temporal focusing characteristics in transparent and scattering media," Opt. Express 19, 4937-4948 (2011).
20. R. M. Mazo, Brownian Motion: Fluctuations, Dynamics, and Applications (Oxford University Press, 2009).
21. E. Papagiakoumou, A. Bègue, O. Schwartz, D. Oron, and V. Emiliani, "Shaped two-photon excitation deep inside scattering tissue," submitted for publication.
22. C.-Y. Chung, K.-C. Cho, C.-C. Chang, W.-C. Yen, and S.-J. Chen, "Adaptive optics system with liquid crystal phase-shift interferometer," Appl. Opt. 45, 3409-3414 (2006). (Pubitemid 44021510)
23. M. A. A. Neil, R. Juškaitis, and T. Wilson, "Method of obtaining optical sectioning by using structured light in a conventional microscope," Opt. Lett. 22, 1905-1907 (1997). (Pubitemid 127598999)
24. T. Wilson, M. A. A. Neil, and R. Juškaitis, "Real-time three-dimensional imaging of macroscopic structures," J. Microsc. 191, 116-118 (1998). (Pubitemid 28378435)
25. S. T. Hess, T. P. K. Gitirajan, and M. D. Mason, "Ultra-high Resolution imaging by fluorescence photoactivation localization microscopy," Biophys. J. 91, 4258-4272 (2006). (Pubitemid 44887284)
26. E. Betzig, G. H. Patterson, R. Sougrat, O. W Lindwasser, S. Olenych, J. S. Bonifacino, M. W. Davidson, J. Lippincott-Schwartz, and H. F. Hess, "Imaging intracellular fluorescent proteins at nanometer resolution," Science 313, 1642-1645 (2006). (Pubitemid 44414039)
27. M. J Rust, M. Bates, and X. Zhuang, "Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM)," Nat. Methods 3, 793-795 (2006). (Pubitemid 44445825)
28. D. Lim, K. K. Chu, and J. Mertz, "Wide-field fluorescence sectioning with hybrid speckle and uniformillumination microscopy," Opt. Lett. 33, 1819-1921 (2008).