Real-time two-photon confocal microscopy using a femtosecond, amplified Ti:sapphire system

Journal of Microscopy

Volumn 181, Issue 3, 1996, Pages 253-259

  Brakenhoff, G J ^a   Squier, J ^b   Norris, T ^b   Bliton, A C ^c   Wade, M H ^c   Athey, B ^d  

^a UNIVERSITY OF AMSTERDAM   (Netherlands)

^b UNIVERSITY OF MICHIGAN   (United States)

^c Meridian Instruments Inc ^*  (United States)

^d UNIVERSITY OF MICHIGAN MEDICAL SCHOOL   (United States)

Author keywords

Bilateral; Confocal; Real time imaging; Sectioning; Three dimensional imaging; TPA; Two photon fluorescence

Indexed keywords

SAPPHIRE;

BILATERAL; CONFOCAL; REAL- TIME; REALTIME IMAGING; SECTIONING; THREE DIMENSIONAL IMAGING; TI:SAPPHIRE; TPA; TWO PHOTON FLUORESCENCE; TWO PHOTON IMAGING;

PHOTONS;

ANIMAL CELL; ARTICLE; FLUORESCENCE; IMAGING; MICROSCOPY; NONHUMAN; PRIORITY JOURNAL; SPERM; SWINE;

EID: 0029866469     PISSN: 00222720     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2818.1996.97379.x     Document Type: Article

References (16)

1. Agart, D.A., Hiraoha, Y., Shaw, P. & Sedat, J.W. (1989) Microscopy in three dimensions. Methods Cell Biol. 30, 353-377.
2. Brakenhoff, van der Voort, G.H.T.M., van Spronsen, E.A., Linnemans, W.A.M. & Nanninga, N. (1985) Three-dimensional chromatin distribution in neuroblastoma nuclei shown by confocal scannong laser microscopy. Nature, 317, 748-749
3. Brakenhoff, G.J. & Visscher, K. (1992) Confocal imaging with bilateral scanning and array detectors. J. Microsc. 165, 139-146.
4. Brakenhoff, G.J. & Visscher, K. (1993) Imaging modes for bilateral confocal scanning microscopy. J. Microsc. 171, 17-26.
5. Brakenhoff, G.J. & Visscher, K. (1994) Real-time confocal microscopy. Proc. SPIE, 2184, 93-100.
6. Denk, W., Strickler, J.H. & Webb, W.W. (1990) Two-photon laser scanning fluorescence microscopy. Science, 248, 73-76.
7. Denk, W., Delaney, K.R., Gelperin, A., Kleinfeld, D., Strowbridge, B.W., Tank, D.W. & Yuste, R. (1994) Anatomical and functional imaging of neurons using 2-photon laser scanning microscopy. J. Neurosci. Methods, 54, 151-161.
8. Denk, W., Piston, D.W. & Webb, W.W. (1995) Two-photon molecular excitation in laser-scanning microscopy. Handbook of Biological Confocal Microscopy, 2nd edn (ed. by J. B. Pawley), pp. 445-457. Plenum Press, New York.
9. Deitche, J., Kempe, M. & Rudolph, W. (1994) Resolution in nonlinear laser scanning microscopy. J. Microsc. 174, 69-73.
10. Hallgren, R.C. & Buchholz, C. (1992) Improved solid surface rendering with the simulated fluorescence process (SFP) algorithm. J. Microsc. 166, RP3-RP4.
11. Weast, R.C. (ed.) (1974) Handbook of Chemistry and Physics, 55th edn. CRC Press, Cleveland, OH.
12. Rhee, J.-K., Sosnowski, T.S. & Norris, T.B. (1994) Chirped-pulse amplification of 85-fs pulses at 250 kHz with third order dispersion compensationby use of holographic transmission gratings. Opt. Lett. 19, 1550-1552.
13. Sheppard, C.J.R. & Gu, M. (1990) Image formation in two-photon fluorescence microscopy. Optik. 86, 104-106.
14. van der Voort, H.T.M., Brakenhoff, G.J. & Baarslag, M.W. (1989) Three-dimensional imaging in fluorescence by confocal scanning microscopy. J. Microsc. 153, 125-132
15. Wilson, T. & Sheppard, C. (1984) Theory and Practice of Scanning Optical Microscopy. Academic Press, London.
16. Wilson, T. & Hewlett, S.J. (1990) Imaging in scanning microscopes with slit-shaped detectors. J. Microsc. 160, 115-139.