Purification and cytotoxicity of tag-free bioengineered spider silk proteins

Journal of Biomedical Materials Research - Part A

Volumn 101 A, Issue 2, 2013, Pages 456-464

  Dams Kozlowska, Hanna ^a,b   Majer, Agnieszka ^c   Tomasiewicz, Paulina ^c   Lozinska, Jolanta ^c   Kaplan, David L ^d   Mackiewicz, Andrzej ^a,b,c  

^a GREATER POLAND CANCER CENTRE   (Poland)

^b POZNAN UNIVERSITY OF MEDICAL SCIENCES   (Poland)

^c BioContract   (Poland)

^d Tufts University   (United States)

Author keywords

Bioengineering; Cytotoxicity; Immunogenicity; Spider silk; Tag free purification

Indexed keywords

BIODEGRADATION RATE; BIOMEDICAL APPLICATIONS; CYTOTOXIC; HUMAN USE; IMMUNOGENICITY; MICROCAPSULES; MORPHOLOGICAL FORMS; NEPHILA CLAVIPES; SPIDER SILK PROTEIN; SPIDER SILKS;

ACID RESISTANCE; BIODEGRADATION; BIOLOGICAL MATERIALS; CLONING; CYTOTOXICITY; ESCHERICHIA COLI; MEDICAL APPLICATIONS; ORGANIC ACIDS; PROTEINS; PURIFICATION; SYSTEMS SCIENCE;

SCAFFOLDS (BIOLOGY);

BIOMATERIAL; CARBOXYLIC ACID; CASPASE 3; ESCHERICHIA COLI ENDOTOXIN; LACTATE DEHYDROGENASE; PROTEIN; PROTEIN TAG; SPIDER SILK PROTEIN; SPIDROIN 1; TUMOR NECROSIS FACTOR ALPHA; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ANIMAL CELL; APOPTOSIS; ARTICLE; BIODEGRADATION; BIOENGINEERING; CELL GROWTH; CELL VIABILITY; CYTOTOXICITY; ESCHERICHIA COLI; EXPRESSION VECTOR; FIBROBLAST; FILM; IMMUNOGENICITY; IN VITRO STUDY; MACROPHAGE ACTIVATION; MITOCHONDRION; MOLECULAR CLONING; NEPHILA CLAVIPES; NONHUMAN; PROTEIN EXPRESSION; PROTEIN MOTIF; PROTEIN PURIFICATION;

AMINO ACID SEQUENCE; ANIMALS; BIOENGINEERING; CASPASE 3; CELL DEATH; CELL PROLIFERATION; FIBROBLASTS; FIBROINS; HUMANS; L-LACTATE DEHYDROGENASE; MACROPHAGE ACTIVATION; MACROPHAGES; MICE; MITOCHONDRIA; MOLECULAR SEQUENCE DATA; NIH 3T3 CELLS; RECOMBINANT PROTEINS; TUMOR NECROSIS FACTOR-ALPHA;

EID: 84872682883     PISSN: 15493296     EISSN: 15524965     Source Type: Journal    
DOI: 10.1002/jbm.a.34353     Document Type: Article

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