Acid extraction and purification of recombinant spider silk proteins

Biomacromolecules

Volumn 5, Issue 5, 2004, Pages 1849-1852

  Mello, Charlene M ^a   Soares, Jason W ^a   Arcidiacono, Steven ^a   Butler, Michelle M ^b  

^a US Army Research Development and Engineering Command   (United States)

^b MICROBIOTIX INC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BIOPOLYMERS; CHROMATOGRAPHIC ANALYSIS; ION EXCHANGE; PROTEINS; PURIFICATION; SOLVENT EXTRACTION;

ACID EXTRACTION; ION EXCHANGE CHROMATOGRAPHY; SOLUBILIZATION;

SILK;

BIOPOLYMER; CARBOXYLIC ACID; HYBRID PROTEIN; RECOMBINANT PROTEIN; SILK PROTEIN; UNCLASSIFIED DRUG;

AFFINITY CHROMATOGRAPHY; AMINO ACID SEQUENCE; ARTICLE; CONTROLLED STUDY; CULTURE TECHNIQUE; EXTRACTION; ION EXCHANGE CHROMATOGRAPHY; METHODOLOGY; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN ISOLATION; PROTEIN PURIFICATION; PROTEIN STABILITY; PROTEIN SYNTHESIS; REPRODUCIBILITY; SOLUBILIZATION; SPIDER;

ANIMALS; CHROMATOGRAPHY, ION EXCHANGE; FIBROINS; RECOMBINANT PROTEINS;

ARANEAE;

EID: 5044221153     PISSN: 15257797     EISSN: None     Source Type: Journal    
DOI: 10.1021/bm049815g     Document Type: Article

References (16)

1. Prince, J. T.; McGrath, K. P.; DiGirolamo, C. M.; Kaplan, D. Biochemistry 1995, 34, 10879.
2. Arcidiacono, S.; Mello, C.; Kaplan, D.; Cheley, S.; Bayley, H. Appl. Microbiol. Biotechnol. 1998, 49, 31.
3. Marsh, R. E.; Corey, R. B.; Pauling, L. Biochim. Biophys. Acta 1955, 16, 1.
4. Lucas, F. Discovery, 1964, 25, 20.
5. Andersen, S. O. Comp. Biochem. Physiol. 1970, 35, 705.
6. Mello, C. M.; Yeung, B.; Senecal, K.; Vouros, P.; Kaplan, D. L. In Silk Polymers, Material Science and Biotechnology; Kaplan, D. L., Adams, W. W., Farmer, B., Viney, C., Eds.; ACS Symposium Series 544; American Chemical Society: Washington, DC, 1994; pp 67-79.
7. Lewis, R. V.; Hinman, M.; Kothakota, S.; Fournier, M. J. Protein Expression Purif. 1996, 7, 400.
8. Tillinghast, E. K.; Kavanaugh, E. J. J. Exp. Zool. 1977, 202, 213.
9. Seidel, A.; Liivak, O.; Jelinski, L. W. Macromolecules 1998, 31, 6733.
10. Kaplan, D. L.; Lombardi, S. J.; Muller, W. J.; Fossey, S. A. In BioMaterials: Novel Materialsfrom Biological Sources; Byrom, D., Ed.; Stockton Press: New York, 1991; pp 1-53.
11. Cunniff, P. M.; Fossey, S. A.; Auerbach, M. A.; Song, J. W. In Silk Polymers: Materials Science and Biotechnology; Kaplan, D. L., Adams, W. W., Farmer, B., Viney, C., Eds.; ACS Symposium series 544; American Chemical Society: Washington, DC, 1994; pp 234-251.
12. Fahnestock, S. R.; Irwin, S. L. Appl. Microbiol. Biotechnol. 1997, 47, 23.
13. Fahnestock, S. R.; Bedzyk, L. A. Appl. Microbiol. Biotechnol. 1997, 47, 33.
14. Scheller, J.; Guhrs, K.-H.; Grosse, F.; Conrad, U. Nature Biotechnol. 2001, 19, 573.
15. Arcidiacono, S.; Mello, C.; Butler, M.; Welsh, E.; Soares, J. W.; Ziegler, D.; Allen, A.; Laue, T.; Chase, S. Macromolecules 2002, 35, 1262.
16. Gill, S. C.; von Hipple, P. H. Anal. Biochem. 1989, 28, 319.