Establishment of a rapid and scalable gene expression system in livestock by site-specific integration

Gene

Volumn 515, Issue 2, 2013, Pages 367-371

  Yu, Huiqing ^a,b   Wang, Xuebin ^b   Zhu, Li ^b   He, Zhuzi ^b   Liu, Guohui ^b   Xu, Xujun ^b   Chen, Jianquan ^b   Cheng, Guoxiang ^a,b  

^a SHANGHAI JIAO TONG UNIVERSITY SCHOOL OF MEDICINE   (China)

^b Shanghai Transgenic Research Center   (China)

Author keywords

Flp mediated recombination; Gene targeting; Livestock genome; Somatic cell based transgene

Indexed keywords

COLLAGEN A1; ENHANCED GREEN FLUORESCENT PROTEIN; PROCOLLAGEN; RECOMBINASE; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; CELL CLONE; CONTROLLED STUDY; CYTOMEGALOVIRUS; EMBRYO DEVELOPMENT; EXPRESSION VECTOR; GENE EXPRESSION; GENE EXPRESSION SYSTEM; GENE FUNCTION; GENE LOCUS; GENE REPLACEMENT THERAPY; GENE TARGETING; GENE VECTOR; GENETIC MANIPULATION; GENOME; GOAT; HOMOLOGOUS RECOMBINATION; LIVESTOCK; MOLECULAR CLONING; MOLECULAR DOCKING; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; REJUVENATION; REPORTER GENE; SITE DIRECTED MUTAGENESIS; SOMATIC CELL; TRANSGENE; TRANSGENICS;

ANIMALS; ANIMALS, GENETICALLY MODIFIED; CELLS, CULTURED; CLONING, MOLECULAR; COLLAGEN TYPE I; EMBRYO CULTURE TECHNIQUES; GENES, REPORTER; GENETIC ENGINEERING; GENETIC VECTORS; GOATS; GREEN FLUORESCENT PROTEINS; LIVESTOCK; MUTAGENESIS, INSERTIONAL; RECOMBINANT PROTEINS;

ANIMALIA; CAPRA HIRCUS;

EID: 84872502443     PISSN: 03781119     EISSN: 18790038     Source Type: Journal    
DOI: 10.1016/j.gene.2012.10.017     Document Type: Article

References (25)

1. Beard C., Hochedlinger K., Plath K., Wutz A., Jaenisch R. Efficient method to generate single-copy transgenic mice by site-specific integration in embryonic stem cells. Genesis 2006, 44:23-28.
2. Dai Y., et al. Targeted disruption of the alpha1,3-galactosyltransferase gene in cloned pigs. Nat. Biotechnol. 2002, 20:251-255.
3. Denning C., et al. Deletion of the alpha(1,3)galactosyl transferase (GGTA1) gene and the prion protein (PrP) gene in sheep. Nat. Biotechnol. 2001, 19:559-562.
4. Denning C., Dickinson P., Burl S., Wylie D., Fletcher J., Clark A.J. Gene targeting in primary fetal fibroblasts from sheep and pig. Cloning Stem Cells 2001, 3:221-231.
5. Dymecki S.M. Flp recombinase promotes site-specific DNA recombination in embryonic stem cells and transgenic mice. Proc. Natl. Acad. Sci. U. S. A. 1996, 93:6191-6196.
6. Golding M.C., Long C.R., Carmell M.A., Hannon G.J., Westhusin M.E. Suppression of prion protein in livestock by RNA interference. Proc. Natl. Acad. Sci. U. S. A. 2006, 103:5285-5290.
7. Jagle U., Gasser J.A., Muller M., Kinzel B. Conditional transgene expression mediated by the mouse beta-actin locus. Genesis 2007, 45:659-666.
8. Jang G., et al. An approach for producing transgenic cloned cows by nuclear transfer of cells transfected with human alpha 1-antitrypsin gene. Theriogenology 2006, 65:1800-1812.
9. Kuroiwa Y., et al. Sequential targeting of the genes encoding immunoglobulin-mu and prion protein in cattle. Nat. Genet. 2004, 36:775-780.
10. Lacroix C., et al. FLP/FRT-mediated conditional mutagenesis in pre-erythrocytic stages of Plasmodium berghei. Nat. Protoc. 2011, 6:1412-1428.
11. Lai L., et al. Production of alpha-1,3-galactosyltransferase knockout pigs by nuclear transfer cloning. Science 2002, 295:1089-1092.
12. Laible G., Alonso-Gonzalez L. Gene targeting from laboratory to livestock: current status and emerging concepts. Biotechnol. J. 2009, 4:1278-1292.
13. Marques M.M., Thomson A.J., McCreath K.J., McWhir J. Conventional gene targeting protocols lead to loss of targeted cells when applied to a silent gene locus in primary fibroblasts. J. Biotechnol. 2006, 125:185-193.
14. McCreath K.J., Howcroft J., Campbell K.H., Colman A., Schnieke A.E., Kind A.J. Production of gene-targeted sheep by nuclear transfer from cultured somatic cells. Nature 2000, 405:1066-1069.
15. Premsrirut P.K., et al. A rapid and scalable system for studying gene function in mice using conditional RNA interference. Cell 2011, 145:145-158.
16. Salamone D., et al. High level expression of bioactive recombinant human growth hormone in the milk of a cloned transgenic cow. J. Biotechnol. 2006, 124:469-472.
17. Schnieke A.E., et al. Human factor IX transgenic sheep produced by transfer of nuclei from transfected fetal fibroblasts. Science 1997, 278:2130-2133.
18. Thomson A.J., Marques M.M., McWhir J. Gene targeting in livestock. Reprod. Suppl. 2003, 61:495-508.
19. Tong J., et al. Production of recombinant human lysozyme in the milk of transgenic pigs. Transgenic Res. 2011, 20:417-419.
20. Xu Y., et al. Excision of selectable genes from transgenic goat cells by a protein transducible TAT-Cre recombinase. Gene 2008, 419:70-74.
21. Yanagawa Y., et al. Enrichment and efficient screening of ES cells containing a targeted mutation: the use of DT-A gene with the polyadenylation signal as a negative selection maker. Transgenic Res. 1999, 8:215-221.
22. Yang P., et al. Cattle mammary bioreactor generated by a novel procedure of transgenic cloning for large-scale production of functional human lactoferrin. PLoS One 2008, 3:e3453.
23. Yu G., et al. Functional disruption of the prion protein gene in cloned goats. J. Gen. Virol. 2006, 87:1019-1027.
24. Zhu C., et al. Production of Prnp -/- goats by gene targeting in adult fibroblasts. Transgenic Res. 2009, 18:163-171.
25. Zou X., et al. Generation of cloned goats (Capra hircus) from transfected foetal fibroblast cells, the effect of donor cell cycle. Mol. Reprod. Dev. 2002, 61:164-172.